Identify the wrong statement with regard to Restriction Enzymes.a)They...
Restriction Enzymes
- Restriction enzymes are enzymes that cut the strand of DNA at specific recognition sites.
- These enzymes are useful in genetic engineering for cutting DNA into fragments that can be manipulated and studied.
- Sticky ends are the name given to the single-stranded overhangs that are created when a restriction enzyme cuts DNA at a palindromic site.
- These sticky ends can be joined by using DNA ligases to create recombinant DNA molecules.
- Each restriction enzyme recognizes and cuts a specific DNA sequence, usually one that is palindromic, meaning it reads the same backwards as forwards.
- Therefore, the statement that is wrong is option 'C', which states that sticky ends cannot be joined by using DNA ligases. This is incorrect, as DNA ligases can join these ends together to create recombinant DNA molecules.
Identify the wrong statement with regard to Restriction Enzymes.a)They...
Sticky ends contain free or hanging or unpaired nitrogen bases which can pair to complementary bases present on other DNA segment required to create a recombinant DNA. A ligase is required in absence of sticky ends to join together two segments of DNA. So, the correct answer is 'Sticky ends can be joined by using DNA ligases.'