Because we have to observe that carbon di oxide is released in this process, but if we use leaves than it utilise carbon di oxide for photosynthesis and hence we are unable to test weather CO2 is released or not. So we uses seed in this experiment

Respiration in Plants: Leaves vs Seeds

Introduction:
Respiration is a vital metabolic process in plants, similar to the respiration process in animals. It involves the exchange of gases, primarily oxygen and carbon dioxide, to provide energy for various cellular activities. While respiration primarily occurs in leaves, it also takes place in other plant parts such as stems, roots, flowers, and fruits.

Respiration in Leaves:
Leaves are the primary organs responsible for photosynthesis, which is the process of converting sunlight into chemical energy. However, leaves also play a significant role in respiration. They contain numerous tiny pores called stomata that facilitate gas exchange between the plant and its environment. During respiration, oxygen enters the leaf through these stomata, while carbon dioxide produced as a byproduct of respiration is released back into the atmosphere.

Why Not Use Leaves Instead of Seeds in the Experiment?

1. Accessibility:
Seeds are more accessible and readily available for experimentation compared to leaves. They can be easily obtained from various sources, including plant nurseries, markets, or harvested from mature plants. On the other hand, collecting a sufficient number of leaves for an experiment may require extensive sampling or damage to the plant itself.

2. Consistency and Control:
Using seeds instead of leaves allows for better control and consistency in the experimental conditions. Seeds can be selected from the same plant species, ensuring genetic uniformity and minimizing variations due to different leaf characteristics. This consistency is crucial when comparing the respiration rates between different samples or treatments.

3. Growth Stage:
Seeds provide the advantage of being at a similar growth stage, making the experimental results more reliable. Leaves, on the other hand, may have different ages, sizes, and physiological states, which can affect their respiration rates. Using seeds ensures that all samples are in a comparable growth stage, minimizing confounding factors.

4. Storage and Transport:
Seeds are generally easier to store and transport compared to leaves. They have a longer shelf life and can be stored under appropriate conditions until needed for an experiment. Leaves, however, are more perishable and require careful handling to maintain their viability, which can be challenging, especially for large-scale experiments or when working with delicate or sensitive plant species.

Conclusion:
Although respiration occurs in leaves and other plant parts, using seeds instead of leaves in experiments offers several advantages. These include accessibility, consistency and control, comparable growth stages, and ease of storage and transport. By utilizing seeds, researchers can conduct experiments with greater precision, reliability, and reproducibility, ultimately enhancing our understanding of plant respiration and its role in various physiological processes.