The three most important Mendel’s Laws or principles of inheritance are listed below:
1. Law of dominance:
When Mendel crossed a true-breeding red flowered plant with a true breeding white flowered one, the progeny was found to be red coloured.
The white colour suppressed and the red colour dominated. Mendel called such traits as redness of flowers dominant and their alternatives, such as whiteness, recessive. All the seven characters in peas studied by Mendel behaved in this way, one of each pair of contrasting traits appearing to be dominant and the other recessive.
2. Law of segregation:
Mendel demonstrated that a hybrid between two different varieties possesses both types of parental factors, which subsequently separate or segregate in the gametes. This is known as law of segregation. In contrast to the uniformity of the first generation hybrids, the second generation produced by self-fertilization of the F1 red flowered plants which consist of two different kinds of plants—red ones like the red grandparent and white ones like the white grandparent.
Mendel counted the numbers of individuals with each of the differentiating characters which reappeared by segregation in F2. In the experiment for flower colour, for example, he raised 929 F2 plants and found that 705 of them bore red flowers and 224 bore white flowers. The simple ratio was found that 3/4 of the F2 resembled the dominant grandparent and 1/4 resembled the recessive one, i.e., 3:1.
On the basis of his experiments, Mendel concluded as follows: contrasting characters, such as the red and white flower colours in peas, are determined by something that a transmitted from parents to offspring in sex cells, or gametes. This something of Mendel is now called a gene.
The different genes, for different flower colours or for round wrinkled seeds, do not blend, contaminate or affect each other while they are together in the hybrid. These different genes segregate, separate pure and uncontaminated, pass into different gametes formed by a hybrid and then go to different individuals in the offspring of the hybrid.
To understand how the genes are transmitted and distributed they are symbolized by letters. The dominant genes are provided with capital letters and their recessive alternatives by corresponding small letters. For example, in the cross of red and white peas R stand for the gene for red flowers and r for the alternative, or allelic, form of this gene, which gives white flowers R and r are allelic genes or alleles.
3. Law of independent assortment:
‘When two pairs of independent alleles enter into combination in the F2, they exhibit independent dominant effects. In the formation of gametes the law of segregation operates but the factors assort themselves independently at random and freely.
Dihybrid and monohybrid crosses:
Mendel studied seven pairs of characters in peas. These were—seed colour, seed surface, flower colour, plant height, colour of unripe pods, pod shape and position of flowers. One of his crosses was between a pea plant with round and yellow seed and one with wrinkled and green ones.
This type of cross, which involves two character differences separable in inheritance is called a Dihybrid cross and a cross which involves a single pair of alleles is monohybrid. The hybrids of F1 possessed yellow and round seeds, since the yellow colour is dominant over the green and the round shape is dominant over the wrinkled. When F1 hybrids were Selfed, an F2 generation was obtained consisting of 556 seeds of the following types:
315 round yellow
108 round green
101 round yellow
032 round green
In terms of proportion, these numbers are very close to a 9: 3: 3: 1 ratio
P1: round � wrinkled Yellow � green
F1: round Yellow
F2: 423 round: 133 wrinkled 416 yellow: 140 green
Or about or about
3/4 round: 1/4 wrinkled 3/4 yellow: 1/4 green
The F2 ratios therefore fit close to the ratios expected from crosses involving single gene pairs in which dominance occurs. When these two sets of results are combined in a single Dihybrid cross, one finds that each gene pair acts independently of the other.