Read the given statements and select the correct option.Statement 1: T...
Statement 1: The tumour-inducing plasmid (Ti plasmid) acts as a cloning vector in recombinant DNA technology.
Statement 2: The Ti plasmid which is used in the mechanisms of delivering genes to a cell remains pathogenic.
Explanation:
Recombinant DNA technology involves the manipulation of DNA to create new combinations of genetic material. One of the important tools in this technology is the cloning vector, which is used to carry and transfer desired DNA sequences into a host organism. A cloning vector should possess certain characteristics such as the ability to self-replicate, the presence of a selectable marker, and the ability to accommodate the foreign DNA.
Statement 1: The tumour-inducing plasmid (Ti plasmid) acts as a cloning vector in recombinant DNA technology.
The Ti plasmid is derived from the soil bacterium Agrobacterium tumefaciens, which has the ability to transfer a part of its DNA into plant cells and induce the formation of tumors. In recombinant DNA technology, the Ti plasmid has been extensively studied and modified to act as a cloning vector. It can be modified to remove the tumor-inducing genes and replace them with the desired genes of interest. This modified Ti plasmid can then be introduced into Agrobacterium cells, which can transfer the foreign DNA into the plant cells. Therefore, statement 1 is correct.
Statement 2: The Ti plasmid which is used in the mechanisms of delivering genes to a cell remains pathogenic.
While the wild-type Ti plasmid is pathogenic and can induce tumor formation in plants, the modified Ti plasmid used in recombinant DNA technology has had the tumor-inducing genes removed. The modified Ti plasmid used as a cloning vector does not retain its pathogenicity. Therefore, statement 2 is incorrect.
Conclusion:
Based on the above explanation, statement 1 is correct as the Ti plasmid can act as a cloning vector in recombinant DNA technology by transferring foreign DNA into plant cells. However, statement 2 is incorrect as the modified Ti plasmid used in this technology does not retain its pathogenicity. Therefore, the correct option is B) Statement 1 is correct, but statement 2 is incorrect.
Read the given statements and select the correct option.Statement 1: T...
A tumour inducing Ti plasmid of Agrobacterium tumefaciens has been modified into a cloning vector which is not pathogenic to the plants, However, it is still able to use its mechanism to deliver genes of our interest into various plants.
So, the correct answer is 'Statement 1 is correct but statement 2 is incorrect'.
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