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Which technique involves introducing foreign DNA into recipient cells using a fine microsyringe under a microscope?
- a)Transformation.
- b)Transfection.
- c)Electroporation.
- d)Microinjection.
Correct answer is option 'D'. Can you explain this answer?
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Which technique involves introducing foreign DNA into recipient cells ...
Microinjection is a technique that involves directly injecting foreign DNA into recipient cells using a fine microsyringe under a microscope. This precise method is used for genetic engineering.
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Which technique involves introducing foreign DNA into recipient cells ...
Introduction to Microinjection
Microinjection is a highly precise technique used in molecular biology to introduce foreign DNA into recipient cells. This method stands out due to its direct and targeted approach.
Technique Overview
- Microinjection involves the use of a fine glass microsyringe that can inject DNA directly into the cytoplasm or nucleus of a cell.
- Typically performed under a microscope, this method allows researchers to visualize and control the injection process with high precision.
Procedure Steps
- Cell Preparation: Recipient cells are often cultured and prepared for injection, ensuring they are healthy and in the correct phase of the cell cycle.
- Microsyringe Setup: A glass micropipette is filled with the DNA solution and carefully positioned under the microscope.
- Injection Process: The microsyringe is used to penetrate the cell membrane, allowing the foreign DNA to enter the desired compartment of the cell.
Advantages of Microinjection
- Precision: Direct delivery ensures that the DNA reaches the target area without the need for carrier molecules.
- High Efficiency: This technique can achieve high rates of successful genetic modification in cells, especially in single-cell organisms or early-stage embryos.
- Versatility: Microinjection can be used in a variety of cell types, including mammalian, plant, and even bacterial cells.
Applications
- Transgenic Animals: Microinjection is commonly employed in creating transgenic mice and other animals for research purposes.
- Gene Function Studies: Enables researchers to study the effects of specific genes by introducing them into cells and observing resultant phenotypic changes.
In conclusion, microinjection is a critical technique for genetic manipulation and research, offering a direct method to introduce foreign DNA into cells with precision and efficiency.
Microinjection is a highly precise technique used in molecular biology to introduce foreign DNA into recipient cells. This method stands out due to its direct and targeted approach.
Technique Overview
- Microinjection involves the use of a fine glass microsyringe that can inject DNA directly into the cytoplasm or nucleus of a cell.
- Typically performed under a microscope, this method allows researchers to visualize and control the injection process with high precision.
Procedure Steps
- Cell Preparation: Recipient cells are often cultured and prepared for injection, ensuring they are healthy and in the correct phase of the cell cycle.
- Microsyringe Setup: A glass micropipette is filled with the DNA solution and carefully positioned under the microscope.
- Injection Process: The microsyringe is used to penetrate the cell membrane, allowing the foreign DNA to enter the desired compartment of the cell.
Advantages of Microinjection
- Precision: Direct delivery ensures that the DNA reaches the target area without the need for carrier molecules.
- High Efficiency: This technique can achieve high rates of successful genetic modification in cells, especially in single-cell organisms or early-stage embryos.
- Versatility: Microinjection can be used in a variety of cell types, including mammalian, plant, and even bacterial cells.
Applications
- Transgenic Animals: Microinjection is commonly employed in creating transgenic mice and other animals for research purposes.
- Gene Function Studies: Enables researchers to study the effects of specific genes by introducing them into cells and observing resultant phenotypic changes.
In conclusion, microinjection is a critical technique for genetic manipulation and research, offering a direct method to introduce foreign DNA into cells with precision and efficiency.
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Which technique involves introducing foreign DNA into recipient cells using a fine microsyringe under a microscope?a)Transformation.b)Transfection.c)Electroporation.d)Microinjection.Correct answer is option 'D'. Can you explain this answer? for UPSC 2025 is part of UPSC preparation. The Question and answers have been prepared according to the UPSC exam syllabus. Information about Which technique involves introducing foreign DNA into recipient cells using a fine microsyringe under a microscope?a)Transformation.b)Transfection.c)Electroporation.d)Microinjection.Correct answer is option 'D'. Can you explain this answer? covers all topics & solutions for UPSC 2025 Exam. Find important definitions, questions, meanings, examples, exercises and tests below for Which technique involves introducing foreign DNA into recipient cells using a fine microsyringe under a microscope?a)Transformation.b)Transfection.c)Electroporation.d)Microinjection.Correct answer is option 'D'. Can you explain this answer?.
Which technique involves introducing foreign DNA into recipient cells using a fine microsyringe under a microscope?a)Transformation.b)Transfection.c)Electroporation.d)Microinjection.Correct answer is option 'D'. Can you explain this answer? for UPSC 2025 is part of UPSC preparation. The Question and answers have been prepared according to the UPSC exam syllabus. Information about Which technique involves introducing foreign DNA into recipient cells using a fine microsyringe under a microscope?a)Transformation.b)Transfection.c)Electroporation.d)Microinjection.Correct answer is option 'D'. Can you explain this answer? covers all topics & solutions for UPSC 2025 Exam. Find important definitions, questions, meanings, examples, exercises and tests below for Which technique involves introducing foreign DNA into recipient cells using a fine microsyringe under a microscope?a)Transformation.b)Transfection.c)Electroporation.d)Microinjection.Correct answer is option 'D'. Can you explain this answer?.
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