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The sequence of the 5’ and 3’ primers for the following DNA sequence will be:
5’ AATGCGGCAATCGAGTC3’
3’ TTACGCCGTTAGCTCCAG5’
  • a)
    5’ TTACG 3’, 5’ CTGAG 3’
  • b)
    5’ AATGC 3’, 5’ TTACG 3’
  • c)
    5’ AATGC 3’, 5’ GACTC 3’
  • d)
    5’ AGTC 3’ , 5’ TCAG 3’
Correct answer is option 'C'. Can you explain this answer?
Verified Answer
The sequence of the 5’ and 3’ primers for the following DN...
The sequence of the 5’ and 3’ primers for the given DNA sequence will be 5’ AATGC 3’, 5’ GACTC 3’.
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The sequence of the 5’ and 3’ primers for the following DN...
Ans is c because take the 5' to 3' direction of primer and then see the 2nd strand of DNA they both are complementary . now look at the 2nd primer and match the DNA sequence of 1st strand of DNA but turn the whole primer sequence. so the ans is C
5'AATGCGGCAATCGAGTC3'
3'CTCAG5'←primer
3'TTACGCCGTTAGCTCAG5'
5'AATGC3'← primer
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Community Answer
The sequence of the 5’ and 3’ primers for the following DN...
The DNA sequence given is:

5 AATGCGGCAATCGAGTC3
3 TTACGCCGTTAGCTCCAG5

The primer sequence is required to bind to the template DNA in order to initiate the polymerase chain reaction (PCR) amplification process. The 5' primer binds to the template strand at the 5' end and the 3' primer binds to the template strand at the 3' end.

The sequence of the 5' primer is the complement of the 5' end of the template strand, read from left to right. Similarly, the sequence of the 3' primer is the complement of the 3' end of the template strand, read from right to left.

The sequence of the 5 and 3 primers for the given DNA sequence is:

5' AATGC 3'
- The 5' primer binds to the template strand at the 5' end, so it should be the complement of the first few nucleotides of the template strand (AATGC).

5' GACTC 3'
- The 3' primer binds to the template strand at the 3' end, so it should be the complement of the last few nucleotides of the template strand (GACTC).

Therefore, the correct answer is option C: 5' AATGC 3', 5' GACTC 3'.
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Question Description
The sequence of the 5’ and 3’ primers for the following DNA sequence will be:5’ AATGCGGCAATCGAGTC3’3’ TTACGCCGTTAGCTCCAG5’a)5’ TTACG 3’, 5’ CTGAG 3’b)5’ AATGC 3’, 5’ TTACG 3’c)5’ AATGC 3’, 5’ GACTC 3’d)5’ AGTC 3’ , 5’ TCAG 3’Correct answer is option 'C'. Can you explain this answer? for NEET 2025 is part of NEET preparation. The Question and answers have been prepared according to the NEET exam syllabus. Information about The sequence of the 5’ and 3’ primers for the following DNA sequence will be:5’ AATGCGGCAATCGAGTC3’3’ TTACGCCGTTAGCTCCAG5’a)5’ TTACG 3’, 5’ CTGAG 3’b)5’ AATGC 3’, 5’ TTACG 3’c)5’ AATGC 3’, 5’ GACTC 3’d)5’ AGTC 3’ , 5’ TCAG 3’Correct answer is option 'C'. Can you explain this answer? covers all topics & solutions for NEET 2025 Exam. Find important definitions, questions, meanings, examples, exercises and tests below for The sequence of the 5’ and 3’ primers for the following DNA sequence will be:5’ AATGCGGCAATCGAGTC3’3’ TTACGCCGTTAGCTCCAG5’a)5’ TTACG 3’, 5’ CTGAG 3’b)5’ AATGC 3’, 5’ TTACG 3’c)5’ AATGC 3’, 5’ GACTC 3’d)5’ AGTC 3’ , 5’ TCAG 3’Correct answer is option 'C'. Can you explain this answer?.
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