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A cloning vector has two antibiotic resistance genes- for tetracycline and ampicillin. A foreign DNA was inserted into the tetracycline gene. Non-recombinants would survive on the medium containing :
  • a)
    ampicillin but not tetracycline
  • b)
    tetracycline but not ampicillin
  • c)
    both tetracycline and ampicillin
  • d)
    neither tetracycline nor ampicillin
Correct answer is option 'C'. Can you explain this answer?
Most Upvoted Answer
A cloning vector has two antibiotic resistance genes- for tetracycline...
Normally, the genes encoding resistance to antibiotics such as ampicillin, chloramphenicol, tetracycline or kanamycin, etc., are considered useful selectable markers for E. coli.
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Community Answer
A cloning vector has two antibiotic resistance genes- for tetracycline...
Explanation:

Cloning Vector with Antibiotic Resistance Genes:
- The cloning vector contains two antibiotic resistance genes - one for tetracycline and one for ampicillin.
- These genes allow for the selection of bacteria that have taken up the cloning vector during the cloning process.

Insertion of Foreign DNA:
- In this case, a foreign DNA was inserted into the tetracycline resistance gene of the cloning vector.
- This insertion disrupts the functionality of the tetracycline resistance gene.

Survival on Selective Medium:
- Non-recombinants are bacteria that have not taken up the foreign DNA and still have functional tetracycline resistance.
- These non-recombinant bacteria will survive on the medium containing both tetracycline and ampicillin.
- The presence of ampicillin resistance gene allows these non-recombinants to survive on the medium.

Correct Answer:
- Therefore, the correct answer is option 'C' - non-recombinants would survive on the medium containing both tetracycline and ampicillin.
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A cloning vector has two antibiotic resistance genes- for tetracycline and ampicillin. A foreign DNA was inserted into the tetracycline gene. Non-recombinants would survive on the medium containing :a)ampicillin but not tetracyclineb)tetracycline but not ampicillinc)both tetracycline and ampicillind)neither tetracycline nor ampicillinCorrect answer is option 'C'. Can you explain this answer?
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A cloning vector has two antibiotic resistance genes- for tetracycline and ampicillin. A foreign DNA was inserted into the tetracycline gene. Non-recombinants would survive on the medium containing :a)ampicillin but not tetracyclineb)tetracycline but not ampicillinc)both tetracycline and ampicillind)neither tetracycline nor ampicillinCorrect answer is option 'C'. Can you explain this answer? for NEET 2024 is part of NEET preparation. The Question and answers have been prepared according to the NEET exam syllabus. Information about A cloning vector has two antibiotic resistance genes- for tetracycline and ampicillin. A foreign DNA was inserted into the tetracycline gene. Non-recombinants would survive on the medium containing :a)ampicillin but not tetracyclineb)tetracycline but not ampicillinc)both tetracycline and ampicillind)neither tetracycline nor ampicillinCorrect answer is option 'C'. Can you explain this answer? covers all topics & solutions for NEET 2024 Exam. Find important definitions, questions, meanings, examples, exercises and tests below for A cloning vector has two antibiotic resistance genes- for tetracycline and ampicillin. A foreign DNA was inserted into the tetracycline gene. Non-recombinants would survive on the medium containing :a)ampicillin but not tetracyclineb)tetracycline but not ampicillinc)both tetracycline and ampicillind)neither tetracycline nor ampicillinCorrect answer is option 'C'. Can you explain this answer?.
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