One scientist cultured Cladophora in a suspension of Azotobacter and i...
Using a prism he split light into its spectral components and then illuminated a green alga, Cladophora, placed in a suspension of aerobic bacteria. The bacteria were used to detect the sites of O2 evolution. He observed that the bacteria accumulated mainly in the region of blue and red light of the split spectrum.
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One scientist cultured Cladophora in a suspension of Azotobacter and i...
Understanding the Experiment
The scientist's experiment involved culturing Cladophora (a type of green algae) in a suspension of Azotobacter (a nitrogen-fixing bacterium) while illuminating the culture with light split by a prism. The goal was to observe bacterial accumulation in relation to different wavelengths of light.
Light Spectrum and Photosynthesis
- Photosynthetic Activity: Cladophora, like other photosynthetic organisms, utilizes light energy for photosynthesis, converting carbon dioxide and water into glucose and oxygen.
- Wavelengths of Light: Different colors of light (wavelengths) can influence the rate of photosynthesis and, consequently, the growth and distribution of associated bacteria like Azotobacter.
Why Blue and Red Light?
- Blue Light:
- Has a short wavelength and high energy.
- It is crucial for chlorophyll absorption, promoting efficient photosynthesis.
- Red Light:
- Has a longer wavelength, which is also effectively absorbed by chlorophyll.
- This light enhances the photosynthetic process, leading to increased organic matter production, which bacteria can utilize.
Conclusion: Bacterial Accumulation
- The study found that bacteria accumulated mainly in the blue and red light regions because:
- Both wavelengths are optimal for photosynthesis in Cladophora.
- Enhanced photosynthetic activity leads to more organic substrates available for Azotobacter, promoting its growth.
Thus, the correct answer, option 'D', reflects the importance of blue and red light in driving photosynthesis and supporting the growth of associated bacteria in the culture.