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Which of the following is not a desirable feature of a cloning vector?
- a)Presence of origin of replication
- b)Presence of a marker gene
- c)Presence of single restriction enzyme site
- d)Presence of two or more recognition sites
Correct answer is option 'D'. Can you explain this answer?
Verified Answer
Which of the following is not a desirable feature of a cloning vector?...
Option (d) is the correct answer. Cloning vectors are the carriers of the desired gene in the host cell. The features desirable in a cloning vector are :-
- Presence of origin of replication
- Presence of marker genes
- Presence of very few, preferably single recognition site for the commonly used restriction enzymes
Most Upvoted Answer
Which of the following is not a desirable feature of a cloning vector?...
Understanding Cloning Vectors
Cloning vectors are essential tools in molecular biology for replicating and isolating DNA fragments. They possess several key features that make them effective. However, not all features are desirable, particularly the presence of multiple restriction enzyme sites.
Desirable Features of a Cloning Vector
- Presence of Origin of Replication: This is crucial for the vector to replicate within the host cell. It ensures that the cloned DNA is copied during cell division.
- Presence of a Marker Gene: Marker genes, such as antibiotic resistance genes, allow for the selection of successfully transformed cells. This makes it easier to identify which cells have taken up the vector.
- Presence of Single Restriction Enzyme Site: A single unique restriction site is ideal for cloning because it allows for the insertion of a DNA fragment without disrupting the vector’s essential functions. This ensures that the DNA can be easily inserted and later excised if needed.
Why Multiple Recognition Sites Are Undesirable
- Complexity in Cloning: Having two or more recognition sites for different restriction enzymes can complicate the cloning process. It increases the likelihood of unwanted cuts in the vector itself, leading to potential loss of important vector functions.
- Increased Risk of Insertional Mutagenesis: Multiple sites can lead to unintended insertions or deletions, which may disrupt essential genes in the vector, affecting its stability and function.
- Difficulty in Selection: With multiple sites available, it becomes challenging to control which fragment is inserted, complicating the selection of the desired clone.
In summary, while cloning vectors should have critical features like an origin of replication and marker genes, having two or more restriction enzyme sites is not desirable due to its potential to complicate the cloning process.
Cloning vectors are essential tools in molecular biology for replicating and isolating DNA fragments. They possess several key features that make them effective. However, not all features are desirable, particularly the presence of multiple restriction enzyme sites.
Desirable Features of a Cloning Vector
- Presence of Origin of Replication: This is crucial for the vector to replicate within the host cell. It ensures that the cloned DNA is copied during cell division.
- Presence of a Marker Gene: Marker genes, such as antibiotic resistance genes, allow for the selection of successfully transformed cells. This makes it easier to identify which cells have taken up the vector.
- Presence of Single Restriction Enzyme Site: A single unique restriction site is ideal for cloning because it allows for the insertion of a DNA fragment without disrupting the vector’s essential functions. This ensures that the DNA can be easily inserted and later excised if needed.
Why Multiple Recognition Sites Are Undesirable
- Complexity in Cloning: Having two or more recognition sites for different restriction enzymes can complicate the cloning process. It increases the likelihood of unwanted cuts in the vector itself, leading to potential loss of important vector functions.
- Increased Risk of Insertional Mutagenesis: Multiple sites can lead to unintended insertions or deletions, which may disrupt essential genes in the vector, affecting its stability and function.
- Difficulty in Selection: With multiple sites available, it becomes challenging to control which fragment is inserted, complicating the selection of the desired clone.
In summary, while cloning vectors should have critical features like an origin of replication and marker genes, having two or more restriction enzyme sites is not desirable due to its potential to complicate the cloning process.
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Which of the following is not a desirable feature of a cloning vector?...
Option (d) is the correct answer. Cloning vectors are the carriers of the desired gene in the host cell. The features desirable in a cloning vector are :-
- Presence of origin of replication
- Presence of marker genes
- Presence of very few, preferably single recognition site for the commonly used restriction enzymes
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Which of the following is not a desirable feature of a cloning vector?a)Presence of origin of replicationb)Presence of a marker genec)Presence of single restriction enzyme sited)Presence of two or more recognition sitesCorrect answer is option 'D'. Can you explain this answer? for NEET 2025 is part of NEET preparation. The Question and answers have been prepared according to the NEET exam syllabus. Information about Which of the following is not a desirable feature of a cloning vector?a)Presence of origin of replicationb)Presence of a marker genec)Presence of single restriction enzyme sited)Presence of two or more recognition sitesCorrect answer is option 'D'. Can you explain this answer? covers all topics & solutions for NEET 2025 Exam. Find important definitions, questions, meanings, examples, exercises and tests below for Which of the following is not a desirable feature of a cloning vector?a)Presence of origin of replicationb)Presence of a marker genec)Presence of single restriction enzyme sited)Presence of two or more recognition sitesCorrect answer is option 'D'. Can you explain this answer?.
Which of the following is not a desirable feature of a cloning vector?a)Presence of origin of replicationb)Presence of a marker genec)Presence of single restriction enzyme sited)Presence of two or more recognition sitesCorrect answer is option 'D'. Can you explain this answer? for NEET 2025 is part of NEET preparation. The Question and answers have been prepared according to the NEET exam syllabus. Information about Which of the following is not a desirable feature of a cloning vector?a)Presence of origin of replicationb)Presence of a marker genec)Presence of single restriction enzyme sited)Presence of two or more recognition sitesCorrect answer is option 'D'. Can you explain this answer? covers all topics & solutions for NEET 2025 Exam. Find important definitions, questions, meanings, examples, exercises and tests below for Which of the following is not a desirable feature of a cloning vector?a)Presence of origin of replicationb)Presence of a marker genec)Presence of single restriction enzyme sited)Presence of two or more recognition sitesCorrect answer is option 'D'. Can you explain this answer?.
Solutions for Which of the following is not a desirable feature of a cloning vector?a)Presence of origin of replicationb)Presence of a marker genec)Presence of single restriction enzyme sited)Presence of two or more recognition sitesCorrect answer is option 'D'. Can you explain this answer? in English & in Hindi are available as part of our courses for NEET.
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