Assertion (A): The use of alternative selectable markers in recombinan...
- Assertion (A) Analysis: The assertion is true because alternative selectable markers indeed facilitate a more efficient identification process for recombinant colonies, reducing the complexity associated with traditional antibiotic selection methods.
- Reason (R) Analysis: The reason is also true, as it accurately describes how these markers function by enabling visual differentiation through color change.
- Explanation: The reason directly explains the assertion, as the simplification of the identification process is a direct result of the color change method provided by alternative selectable markers.
- Ncert line: Selection of recombinants due to inactivation of antibiotics is a cumbersome procedure because it requires simultaneous plating on two plates having different antibiotics. Therefore, alternative selectable markers have been developed which differentiate recombinants from non-recombinants on the basis of their ability to produce colour in the presence of a chromogenic substrate.
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Assertion (A): The use of alternative selectable markers in recombinan...
Understanding the Assertion and Reason
The assertion and reason provided in the question relate to the use of alternative selectable markers in recombinant DNA technology.
Assertion (A): The use of alternative selectable markers in recombinant DNA technology simplifies the identification of recombinant colonies.
- Selectable markers are crucial in recombinant DNA technology.
- They help distinguish between colonies that contain recombinant DNA and those that do not.
- By using alternative markers, researchers can streamline the identification process.
Reason (R): These markers allow for the differentiation of recombinants from non-recombinants based solely on color change in the presence of a chromogenic substrate.
- Chromogenic substrates react with specific markers to produce a color change.
- This color change serves as a visual indicator of successful recombination.
- It provides a straightforward method for identifying recombinant colonies.
Why Option A is Correct
- Both the assertion and reason are true.
- The assertion accurately reflects that alternative selectable markers simplify the identification process.
- The reason correctly explains how these markers function, specifically highlighting the role of color change in differentiating recombinants from non-recombinants.
Conclusion
- Therefore, option "A" is correct, as both statements are true, and the reason provided is indeed the correct explanation for the assertion.
- This understanding enhances the efficiency of selecting recombinant DNA in laboratory settings, showcasing the importance of these techniques in biotechnology.