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What is the primary function of the origin of replication (ori) in a DNA vector?
- a)To provide a selectable marker.
- b)To initiate DNA replication.
- c)To enhance the stability of the DN
- d)To facilitate transformation into host cells.
Correct answer is option 'B'. Can you explain this answer?
Most Upvoted Answer
What is the primary function of the origin of replication (ori) in a D...
The origin of replication (ori) is crucial for initiating the replication of the DNA within the host cells, ensuring that the DNA is copied during cell division.
Ncert Topic: Cloning Vectors
Ncert line: Origin of replication (ori) : This is a sequence from where replication starts and any piece of DNA when linked to this sequence can be made to replicate within the host cells. This sequence is also responsible for controlling the copy number of the linked DNA
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What is the primary function of the origin of replication (ori) in a D...
Primary Function of the Origin of Replication (ori)
The origin of replication (ori) is a crucial component in DNA vectors used in molecular biology. Its primary function can be summarized as follows:
Initiation of DNA Replication
- The ori serves as a specific sequence where the process of DNA replication begins in a host cell.
- During replication, proteins recognize the ori and bind to it, unwinding the DNA to create a replication bubble.
- This process allows the DNA polymerase enzyme to synthesize new strands of DNA, effectively duplicating the vector and any inserted genetic material.
Importance in Cloning and Gene Expression
- The presence of a functional ori ensures that the vector can replicate independently once inside a suitable host cell, such as bacteria, yeast, or mammalian cells.
- This replication is essential for cloning genes, producing proteins, or creating genetically modified organisms.
Other Functions of DNA Vectors
While option 'B' is correct, it's worth noting that DNA vectors can also contain other elements, such as:
- Selectable Markers: Help identify successfully transformed cells.
- Promoters and Enhancers: Facilitate gene expression.
- Stability Elements: Ensure the maintenance of the vector within the host.
In summary, the primary function of the origin of replication (ori) in a DNA vector is to initiate DNA replication, allowing the vector and its genetic content to be copied and propagated within host cells. This is essential for successful cloning and gene expression studies.
The origin of replication (ori) is a crucial component in DNA vectors used in molecular biology. Its primary function can be summarized as follows:
Initiation of DNA Replication
- The ori serves as a specific sequence where the process of DNA replication begins in a host cell.
- During replication, proteins recognize the ori and bind to it, unwinding the DNA to create a replication bubble.
- This process allows the DNA polymerase enzyme to synthesize new strands of DNA, effectively duplicating the vector and any inserted genetic material.
Importance in Cloning and Gene Expression
- The presence of a functional ori ensures that the vector can replicate independently once inside a suitable host cell, such as bacteria, yeast, or mammalian cells.
- This replication is essential for cloning genes, producing proteins, or creating genetically modified organisms.
Other Functions of DNA Vectors
While option 'B' is correct, it's worth noting that DNA vectors can also contain other elements, such as:
- Selectable Markers: Help identify successfully transformed cells.
- Promoters and Enhancers: Facilitate gene expression.
- Stability Elements: Ensure the maintenance of the vector within the host.
In summary, the primary function of the origin of replication (ori) in a DNA vector is to initiate DNA replication, allowing the vector and its genetic content to be copied and propagated within host cells. This is essential for successful cloning and gene expression studies.
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What is the primary function of the origin of replication (ori) in a DNA vector?a)To provide a selectable marker.b)To initiate DNA replication.c)To enhance the stability of the DNd)To facilitate transformation into host cells.Correct answer is option 'B'. Can you explain this answer? for NEET 2025 is part of NEET preparation. The Question and answers have been prepared according to the NEET exam syllabus. Information about What is the primary function of the origin of replication (ori) in a DNA vector?a)To provide a selectable marker.b)To initiate DNA replication.c)To enhance the stability of the DNd)To facilitate transformation into host cells.Correct answer is option 'B'. Can you explain this answer? covers all topics & solutions for NEET 2025 Exam. Find important definitions, questions, meanings, examples, exercises and tests below for What is the primary function of the origin of replication (ori) in a DNA vector?a)To provide a selectable marker.b)To initiate DNA replication.c)To enhance the stability of the DNd)To facilitate transformation into host cells.Correct answer is option 'B'. Can you explain this answer?.
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