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Which of the following is a common selectable marker used in genetic transformation?
- a)Green fluorescent protein
- b)Chlorophyll
- c)Antibiotic resistance genes
- d)Enzyme activity genes
Correct answer is option 'C'. Can you explain this answer?
Most Upvoted Answer
Which of the following is a common selectable marker used in genetic t...
Common Selectable Marker in Genetic Transformation
In the context of genetic transformation, selectable markers are essential for identifying successfully modified cells. Among the options provided, antibiotic resistance genes are the most common and effective selectable markers.
Why Antibiotic Resistance Genes?
- Functionality: Antibiotic resistance genes provide a survival advantage to transformed cells in the presence of specific antibiotics. Only those cells that have successfully incorporated the resistance gene can survive.
- Examples: Common examples include the kanamycin resistance gene (nptII) and ampicillin resistance gene (bla). Cells that express these genes can grow in media containing the corresponding antibiotics, while untransformed cells will die.
Comparison with Other Options
- Green Fluorescent Protein (GFP): While GFP is a valuable marker for visualizing gene expression, it does not confer a survival advantage in selective media. Therefore, it's not a true selectable marker.
- Chlorophyll: This is not used as a selectable marker in genetic transformation. It is a pigment essential for photosynthesis but does not indicate successful transformation.
- Enzyme Activity Genes: Similar to GFP, these may indicate gene expression but do not provide a mechanism for selection under antibiotic pressure.
Conclusion
Using antibiotic resistance genes as selectable markers simplifies the identification of genetically transformed organisms, making them a cornerstone in genetic engineering and biotechnology. Their ability to enhance survival in selective environments is what sets them apart from other options.
In the context of genetic transformation, selectable markers are essential for identifying successfully modified cells. Among the options provided, antibiotic resistance genes are the most common and effective selectable markers.
Why Antibiotic Resistance Genes?
- Functionality: Antibiotic resistance genes provide a survival advantage to transformed cells in the presence of specific antibiotics. Only those cells that have successfully incorporated the resistance gene can survive.
- Examples: Common examples include the kanamycin resistance gene (nptII) and ampicillin resistance gene (bla). Cells that express these genes can grow in media containing the corresponding antibiotics, while untransformed cells will die.
Comparison with Other Options
- Green Fluorescent Protein (GFP): While GFP is a valuable marker for visualizing gene expression, it does not confer a survival advantage in selective media. Therefore, it's not a true selectable marker.
- Chlorophyll: This is not used as a selectable marker in genetic transformation. It is a pigment essential for photosynthesis but does not indicate successful transformation.
- Enzyme Activity Genes: Similar to GFP, these may indicate gene expression but do not provide a mechanism for selection under antibiotic pressure.
Conclusion
Using antibiotic resistance genes as selectable markers simplifies the identification of genetically transformed organisms, making them a cornerstone in genetic engineering and biotechnology. Their ability to enhance survival in selective environments is what sets them apart from other options.
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Which of the following is a common selectable marker used in genetic t...
Antibiotic resistance genes are commonly used as selectable markers in genetic transformation, allowing the identification of transformed cells.
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Which of the following is a common selectable marker used in genetic transformation?a)Green fluorescent proteinb)Chlorophyllc)Antibiotic resistance genesd)Enzyme activity genesCorrect answer is option 'C'. Can you explain this answer? for NEET 2025 is part of NEET preparation. The Question and answers have been prepared according to the NEET exam syllabus. Information about Which of the following is a common selectable marker used in genetic transformation?a)Green fluorescent proteinb)Chlorophyllc)Antibiotic resistance genesd)Enzyme activity genesCorrect answer is option 'C'. Can you explain this answer? covers all topics & solutions for NEET 2025 Exam. Find important definitions, questions, meanings, examples, exercises and tests below for Which of the following is a common selectable marker used in genetic transformation?a)Green fluorescent proteinb)Chlorophyllc)Antibiotic resistance genesd)Enzyme activity genesCorrect answer is option 'C'. Can you explain this answer?.
Which of the following is a common selectable marker used in genetic transformation?a)Green fluorescent proteinb)Chlorophyllc)Antibiotic resistance genesd)Enzyme activity genesCorrect answer is option 'C'. Can you explain this answer? for NEET 2025 is part of NEET preparation. The Question and answers have been prepared according to the NEET exam syllabus. Information about Which of the following is a common selectable marker used in genetic transformation?a)Green fluorescent proteinb)Chlorophyllc)Antibiotic resistance genesd)Enzyme activity genesCorrect answer is option 'C'. Can you explain this answer? covers all topics & solutions for NEET 2025 Exam. Find important definitions, questions, meanings, examples, exercises and tests below for Which of the following is a common selectable marker used in genetic transformation?a)Green fluorescent proteinb)Chlorophyllc)Antibiotic resistance genesd)Enzyme activity genesCorrect answer is option 'C'. Can you explain this answer?.
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