In which of the following does the recombinant DNA get multiplied?a)In...
The recombinant DNA is multiplied in the host organism, which replicates the DNA as part of its normal cell processes.
In which of the following does the recombinant DNA get multiplied?a)In...
Understanding Recombinant DNA Multiplication
Recombinant DNA technology involves combining DNA from different sources, which can then be multiplied for various applications such as research, medicine, and biotechnology. The correct answer to where recombinant DNA gets multiplied is option 'C', in the host organism.
Why the Host Organism?
- Natural Replication Mechanism: Host organisms, particularly bacteria like E. coli, possess natural mechanisms for DNA replication. When recombinant DNA is introduced into a host, the host’s cellular machinery recognizes it as part of its own genetic material.
- Plasmid Vectors: While plasmid vectors are essential for the initial introduction of recombinant DNA, they rely on the host's cellular machinery to replicate. Plasmids can replicate independently within the host, but it's the host organism that ultimately amplifies the plasmid and the inserted DNA.
- Cell Division: Each time the host organism divides, it copies its entire DNA, including the recombinant DNA. This process results in the exponential multiplication of the recombinant DNA.
Other Options Explained
- In the Lab Environment: Although experiments are conducted in a lab, the actual multiplication occurs within a biological system, not just in the lab.
- In the Plasmid Vector: Plasmids serve as carriers but do not multiply on their own. They depend on the host for replication.
- In the Restriction Enzyme: Restriction enzymes are tools used to cut DNA at specific sequences but do not participate in DNA multiplication.
In summary, the host organism is crucial for the amplification of recombinant DNA, utilizing its cellular processes to ensure the DNA is replicated during cell division.