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Which of the following is NOT considered a useful selectable marker for E. coli?
- a)Ampicillin resistance gene
- b)Chloramphenicol resistance gene
- c)Tetracycline resistance gene
- d)Drug resistance E.coli gene against penicillin
Correct answer is option 'D'. Can you explain this answer?
Most Upvoted Answer
Which of the following is NOT considered a useful selectable marker fo...
The drug resistance gene against penicillin is not typically used as a selectable marker in E. coli, as it does not provide a reliable means of selection compared to other antibiotic resistance genes.
Ncert Topic: Cloning Vectors ( Selectable Marker)
Ncert Line: Normally, the genes encoding resistance to antibiotics such as ampicillin, chloramphenicol, tetracycline or kanamycin, etc., are considered useful selectable markers for E. coli.
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Which of the following is NOT considered a useful selectable marker fo...
Understanding Selectable Markers in E. coli
Selectable markers are essential tools in molecular biology, particularly for the transformation and selection of genetically modified organisms. In the case of E. coli, certain markers are commonly used, while others may not be effective.
Common Selectable Markers
- Ampicillin Resistance Gene: This gene allows E. coli to survive in the presence of ampicillin, making it a widely used selectable marker for plasmids.
- Chloramphenicol Resistance Gene: Similar to the ampicillin resistance gene, this marker enables E. coli to grow in the presence of chloramphenicol, facilitating the selection of transformed cells.
- Tetracycline Resistance Gene: This gene provides E. coli with resistance to tetracycline, allowing for the selection of successfully transformed bacteria.
Why Penicillin Resistance is NOT a Useful Marker
- Drug Resistance E.coli Gene Against Penicillin: While penicillin resistance may seem like a viable option, it is not considered a useful selectable marker in E. coli for several reasons:
- Ineffectiveness in E. coli: E. coli is inherently resistant to penicillin due to its cell wall structure. Therefore, using a penicillin resistance gene does not provide a selection advantage, as E. coli can grow in penicillin-containing media without any genetic modification.
- Lack of Selection Pressure: Since E. coli can already survive in penicillin, it fails to distinguish transformed from non-transformed cells, rendering it ineffective as a selectable marker.
In conclusion, when selecting markers for E. coli, it is crucial to choose those that provide a clear selection advantage, such as ampicillin, chloramphenicol, or tetracycline resistance genes, while avoiding options like penicillin resistance that do not serve this purpose.
Selectable markers are essential tools in molecular biology, particularly for the transformation and selection of genetically modified organisms. In the case of E. coli, certain markers are commonly used, while others may not be effective.
Common Selectable Markers
- Ampicillin Resistance Gene: This gene allows E. coli to survive in the presence of ampicillin, making it a widely used selectable marker for plasmids.
- Chloramphenicol Resistance Gene: Similar to the ampicillin resistance gene, this marker enables E. coli to grow in the presence of chloramphenicol, facilitating the selection of transformed cells.
- Tetracycline Resistance Gene: This gene provides E. coli with resistance to tetracycline, allowing for the selection of successfully transformed bacteria.
Why Penicillin Resistance is NOT a Useful Marker
- Drug Resistance E.coli Gene Against Penicillin: While penicillin resistance may seem like a viable option, it is not considered a useful selectable marker in E. coli for several reasons:
- Ineffectiveness in E. coli: E. coli is inherently resistant to penicillin due to its cell wall structure. Therefore, using a penicillin resistance gene does not provide a selection advantage, as E. coli can grow in penicillin-containing media without any genetic modification.
- Lack of Selection Pressure: Since E. coli can already survive in penicillin, it fails to distinguish transformed from non-transformed cells, rendering it ineffective as a selectable marker.
In conclusion, when selecting markers for E. coli, it is crucial to choose those that provide a clear selection advantage, such as ampicillin, chloramphenicol, or tetracycline resistance genes, while avoiding options like penicillin resistance that do not serve this purpose.
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Which of the following is NOT considered a useful selectable marker for E. coli?a)Ampicillin resistance geneb)Chloramphenicol resistance genec)Tetracycline resistance gened)Drug resistance E.coli gene against penicillinCorrect answer is option 'D'. Can you explain this answer? for NEET 2025 is part of NEET preparation. The Question and answers have been prepared according to the NEET exam syllabus. Information about Which of the following is NOT considered a useful selectable marker for E. coli?a)Ampicillin resistance geneb)Chloramphenicol resistance genec)Tetracycline resistance gened)Drug resistance E.coli gene against penicillinCorrect answer is option 'D'. Can you explain this answer? covers all topics & solutions for NEET 2025 Exam. Find important definitions, questions, meanings, examples, exercises and tests below for Which of the following is NOT considered a useful selectable marker for E. coli?a)Ampicillin resistance geneb)Chloramphenicol resistance genec)Tetracycline resistance gened)Drug resistance E.coli gene against penicillinCorrect answer is option 'D'. Can you explain this answer?.
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