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The component which is not required in PCR is__.
- a)Mg2+
- b)Polymerase
- c)Calcium ions
- d)dNTPs
Correct answer is option 'C'. Can you explain this answer?
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Verified Answer
The component which is not required in PCR is__.a)Mg2+b)Polymerasec)Ca...
- The polymerase chain reaction is a technology in molecular biology used to amplify a single copy or a few copies of a piece of DNA.
- Calcium ions is not required in PCR technology.
Most Upvoted Answer
The component which is not required in PCR is__.a)Mg2+b)Polymerasec)Ca...
Mg is increasing teg polimarese reactivity but calcium is decrease this reactivity so it can not use
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The component which is not required in PCR is__.a)Mg2+b)Polymerasec)Ca...
Explanation:
Polymerase Chain Reaction (PCR) is a laboratory technique used to amplify a specific DNA sequence. It involves several components that are necessary for the reaction to proceed. However, there is one component that is not required in PCR, and that is calcium ions.
PCR components:
1. Template DNA: The DNA sequence to be amplified.
2. Primers: Short DNA sequences that bind to the template DNA and define the boundaries of the amplified sequence.
3. Taq polymerase: A DNA polymerase enzyme that synthesizes new DNA strands from the primers.
4. dNTPs: Deoxynucleoside triphosphates, the building blocks of DNA.
5. Mg2+: Magnesium ions, which are essential for the activity of Taq polymerase.
Why calcium ions are not required in PCR?
Calcium ions are not involved in the PCR reaction. They are not necessary for the activity of Taq polymerase or any other component of the reaction. Therefore, they are not added to the reaction mixture.
Conclusion:
In conclusion, the component that is not required in PCR is calcium ions. PCR requires template DNA, primers, Taq polymerase, dNTPs, and magnesium ions to amplify a specific DNA sequence.
Polymerase Chain Reaction (PCR) is a laboratory technique used to amplify a specific DNA sequence. It involves several components that are necessary for the reaction to proceed. However, there is one component that is not required in PCR, and that is calcium ions.
PCR components:
1. Template DNA: The DNA sequence to be amplified.
2. Primers: Short DNA sequences that bind to the template DNA and define the boundaries of the amplified sequence.
3. Taq polymerase: A DNA polymerase enzyme that synthesizes new DNA strands from the primers.
4. dNTPs: Deoxynucleoside triphosphates, the building blocks of DNA.
5. Mg2+: Magnesium ions, which are essential for the activity of Taq polymerase.
Why calcium ions are not required in PCR?
Calcium ions are not involved in the PCR reaction. They are not necessary for the activity of Taq polymerase or any other component of the reaction. Therefore, they are not added to the reaction mixture.
Conclusion:
In conclusion, the component that is not required in PCR is calcium ions. PCR requires template DNA, primers, Taq polymerase, dNTPs, and magnesium ions to amplify a specific DNA sequence.
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The component which is not required in PCR is__.a)Mg2+b)Polymerasec)Calcium ionsd)dNTPsCorrect answer is option 'C'. Can you explain this answer?
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