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Assertion: The separated DNA fragments can be visualized only after staining the DNA with a compound known as ethidium bromide followed by exposure to UV radiation.


Reason: we can see bright red coloured bands of DNA in a ethidium bromide stained gel exposed to UV light.

  • a)
    Both assertion and reason are correct.

  • b)
    Assertion is correct but reason is incorrect.

  • c)
    Both assertion and reason are incorrect.

  • d)
    Assertion is correct but reason does not explain the assertin.

Correct answer is option 'B'. Can you explain this answer?
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Assertion: The separated DNA fragments can be visualized only after st...
Assertion is correct but reason is incorrect because when DNA stain by Aethidium bromide so we can see orange color bands of DNA.
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Assertion: The separated DNA fragments can be visualized only after st...
Assertion: The separated DNA fragments can be visualized only after staining the DNA with a compound known as ethidium bromide followed by exposure to UV radiation.

Reason: We can see bright red coloured bands of DNA in an ethidium bromide-stained gel exposed to UV light.

Answer:

The correct option is (a) Both assertion and reason are correct.

Explanation:

Separation of DNA fragments is typically performed by gel electrophoresis. In this technique, DNA fragments are loaded onto a gel matrix and subjected to an electric field. The electric field causes the DNA fragments to migrate through the gel matrix and separate based on their size.

However, these separated DNA fragments are not visible to the naked eye. To visualize them, the gel is stained with a compound known as ethidium bromide, which intercalates between the DNA fragments and fluoresces under UV radiation.

Therefore, the reason given for the assertion is correct. Ethidium bromide-stained gels exposed to UV light appear as bright red coloured bands of DNA, with each band representing a different-sized DNA fragment.

Hence, both the assertion and reason are correct.
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Assertion: The separated DNA fragments can be visualized only after staining the DNA with a compound known as ethidium bromide followed by exposure to UV radiation.Reason: we can see bright red coloured bands of DNA in a ethidium bromide stained gel exposed to UV light.a)Both assertion and reason are correct.b)Assertion is correct but reason is incorrect.c)Both assertion and reason are incorrect.d)Assertion is correct but reason does not explain the assertin.Correct answer is option 'B'. Can you explain this answer?
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Assertion: The separated DNA fragments can be visualized only after staining the DNA with a compound known as ethidium bromide followed by exposure to UV radiation.Reason: we can see bright red coloured bands of DNA in a ethidium bromide stained gel exposed to UV light.a)Both assertion and reason are correct.b)Assertion is correct but reason is incorrect.c)Both assertion and reason are incorrect.d)Assertion is correct but reason does not explain the assertin.Correct answer is option 'B'. Can you explain this answer? for NEET 2024 is part of NEET preparation. The Question and answers have been prepared according to the NEET exam syllabus. Information about Assertion: The separated DNA fragments can be visualized only after staining the DNA with a compound known as ethidium bromide followed by exposure to UV radiation.Reason: we can see bright red coloured bands of DNA in a ethidium bromide stained gel exposed to UV light.a)Both assertion and reason are correct.b)Assertion is correct but reason is incorrect.c)Both assertion and reason are incorrect.d)Assertion is correct but reason does not explain the assertin.Correct answer is option 'B'. Can you explain this answer? covers all topics & solutions for NEET 2024 Exam. Find important definitions, questions, meanings, examples, exercises and tests below for Assertion: The separated DNA fragments can be visualized only after staining the DNA with a compound known as ethidium bromide followed by exposure to UV radiation.Reason: we can see bright red coloured bands of DNA in a ethidium bromide stained gel exposed to UV light.a)Both assertion and reason are correct.b)Assertion is correct but reason is incorrect.c)Both assertion and reason are incorrect.d)Assertion is correct but reason does not explain the assertin.Correct answer is option 'B'. Can you explain this answer?.
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