The smallest bands in the agarose gel will be towardsa)Wellsb)Middle of the gelc)Cathoded)AnodeCorrect answer is option 'D'. Can you explain this answer?

Question

Answer

Actually DNA is negatively charged. so. smaller segments of DNA will be found close to the anode.

Answer

DNA is -vely charged and moves towards anode( + vely)  charged as unlike charges attracts each other

Answer

Dna fragments are negatively charged which are seperated by moving towards the anode  refer page 198 biotech

Answer

Anode have positive charge and DNA have negative so smaller DNA fragment will move more and reach to anode

Answer

The smallest bands in the agarose gel will be towards the Anode.

Agarose gel electrophoresis is a widely used technique in molecular biology to separate and analyze DNA fragments of different sizes. It is based on the principle that negatively charged DNA molecules will migrate towards the positive electrode (anode) when an electric field is applied.

Here is a detailed explanation of why the smallest bands will be towards the Anode:

1. Principle of Agarose Gel Electrophoresis:
- Agarose gel is a porous matrix that forms a sieve-like structure when solidified.
- DNA fragments are loaded into wells at one end of the gel, and an electric field is applied across the gel.
- The electric field causes the negatively charged DNA molecules to migrate through the gel towards the positively charged electrode.
- The rate of migration depends on the size and charge of the DNA fragments, with smaller fragments migrating faster than larger ones.

2. Direction of DNA Migration:
- The DNA fragments migrate from the cathode (negative electrode) towards the anode (positive electrode).
- This is because DNA is negatively charged due to the phosphate groups in its backbone, and opposite charges attract in an electric field.
- Therefore, the smaller DNA fragments, which experience less resistance from the gel matrix, will migrate faster and travel further towards the anode compared to larger fragments.

3. Position of Wells and Bands:
- The wells, where the DNA samples are loaded, are typically located at one end of the gel.
- When the electric field is applied, the DNA fragments will migrate away from the wells towards the anode.
- Since the smaller fragments migrate faster, they will reach the anode first and form the smallest bands closer to the anode.
- Conversely, the larger fragments will migrate slower and form larger bands closer to the wells.

Therefore, based on the principles of agarose gel electrophoresis, the smallest bands in the gel will be towards the Anode, where the DNA fragments migrate during the electrophoretic process.

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