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During gel electrophoresis for separation of DNA fragment
- a)Smallest fragment will move to the farthest point towards cathode
- b)Smallest fragment will move to the farthest point towards anode
- c)Largest fragment will move to the farthest point towards cathode
- d)Largest fragment will move to the farthest point towards anode
Correct answer is option 'B'. Can you explain this answer?
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Understanding Gel Electrophoresis
Gel electrophoresis is a technique used to separate DNA fragments based on their size. The process is driven by an electric current that moves negatively charged DNA towards the positive electrode (anode).
Key Principles of Gel Electrophoresis
- Charge of DNA: DNA fragments are negatively charged due to their phosphate backbone. When an electric field is applied, they migrate towards the anode.
- Size Matters: The gel matrix (often agarose or polyacrylamide) acts as a sieve. Smaller fragments can navigate through the pores of the gel more easily than larger ones.
Movement of DNA Fragments
- Smallest Fragments: These fragments can move through the gel matrix with greater ease, allowing them to travel further towards the anode. This is why the smallest DNA fragments end up being the farthest from the well where they were loaded.
- Largest Fragments: In contrast, the larger fragments encounter more resistance as they navigate through the gel. Hence, they do not migrate as far and remain closer to the well.
Conclusion
Thus, in gel electrophoresis, the correct answer is:
- Smallest fragment will move to the farthest point towards anode (Option B).
This principle is vital for applications such as DNA fingerprinting, cloning, and various genetic analyses, as it enables researchers to separate and analyze DNA fragments effectively.
Gel electrophoresis is a technique used to separate DNA fragments based on their size. The process is driven by an electric current that moves negatively charged DNA towards the positive electrode (anode).
Key Principles of Gel Electrophoresis
- Charge of DNA: DNA fragments are negatively charged due to their phosphate backbone. When an electric field is applied, they migrate towards the anode.
- Size Matters: The gel matrix (often agarose or polyacrylamide) acts as a sieve. Smaller fragments can navigate through the pores of the gel more easily than larger ones.
Movement of DNA Fragments
- Smallest Fragments: These fragments can move through the gel matrix with greater ease, allowing them to travel further towards the anode. This is why the smallest DNA fragments end up being the farthest from the well where they were loaded.
- Largest Fragments: In contrast, the larger fragments encounter more resistance as they navigate through the gel. Hence, they do not migrate as far and remain closer to the well.
Conclusion
Thus, in gel electrophoresis, the correct answer is:
- Smallest fragment will move to the farthest point towards anode (Option B).
This principle is vital for applications such as DNA fingerprinting, cloning, and various genetic analyses, as it enables researchers to separate and analyze DNA fragments effectively.
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During gel electrophoresis for separation of DNA fragmenta)Smallest fragment will move to the farthest point towards cathodeb)Smallest fragment will move to the farthest point towards anodec)Largest fragment will move to the farthest point towards cathoded)Largest fragment will move to the farthest point towards anodeCorrect answer is option 'B'. Can you explain this answer?
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