If KOH solution isn't get kept in the conical flask, then the vacuum atmosphere won't be get develope in the flask and the water level won't be raise

**Impact of Forgetting to Keep KOH in the Conical Flask on the Experimental Result**

When conducting an experiment to measure the release of CO2 during respiration, it is crucial to include a vial of KOH (potassium hydroxide) in the conical flask. The role of KOH is to absorb the released CO2, preventing it from affecting the experimental outcome. However, if one forgets to include the vial of KOH, the result of the experiment will vary significantly. Let's explore the details of this variation.

**1. Increased CO2 Concentration:**
Without the presence of KOH, the released CO2 will accumulate in the conical flask, leading to a higher concentration of CO2 in the flask. As a result, the measured volume of CO2 will be greater compared to when KOH is present. This will introduce an error in the results, making them inaccurate and unreliable.

**2. Altered pH Level:**
The absence of KOH in the conical flask will also affect the pH level of the solution. Respiration is a process that generates carbonic acid (H2CO3) as a byproduct. In the presence of KOH, this carbonic acid reacts with the hydroxide ions (OH-) from KOH to form water (H2O) and carbonate ions (CO32-). This reaction helps maintain a stable pH level in the solution during the respiration process. However, without KOH, the carbonic acid will accumulate in the solution, causing a decrease in pH. This change in pH can disrupt the respiration process and affect the overall experimental outcome.

**3. Inaccurate Measurement of Respiration Rate:**
The primary objective of measuring CO2 release during respiration is to determine the respiration rate. The absence of KOH will lead to an inaccurate measurement of this rate. Since the accumulated CO2 in the conical flask will not be absorbed, it will contribute to an overestimation of the respiration rate. This error can misrepresent the actual respiration rate and hinder the validity of the experimental data.

**Conclusion:**
Forgetting to include the vial of KOH in the conical flask during an experiment to measure CO2 release during respiration will significantly impact the results. It will lead to an increased concentration of CO2, altered pH levels, and inaccurate measurement of the respiration rate. Therefore, it is crucial to ensure the inclusion of KOH to absorb the released CO2 and maintain the experimental conditions necessary for obtaining reliable and accurate results.