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The American company which is involved in the manufacturing of Humulin is
  • a)
    Elixir
  • b)
    Ranbaxy
  • c)
    Prohealth
  • d)
    Eli Lily
Correct answer is option 'D'. Can you explain this answer?

Harshitha Dey answered
In 1983, the American company Eli Lily prepared two DNA sequences corresponding to A and B and introduced into plasmid of E.coli to produce insulin chain.

Which of the following is not applicable for a biopatent?
  • a)
    Formation of new products
  • b)
    Unearthing the DNA sequences
  • c)
    Discovery of cell lines
  • d)
    Using the existing technology to produce new products
Correct answer is option 'C'. Can you explain this answer?

Geetika Shah answered
A biological patent is a patent on an invention in the field of biology that by law allows the patent holder to exclude others from making, using, selling, or importing the protected invention for a limited period of time. Hence, the correct option is c.

 Insect resistance transgenic cotton has been produced by inserting a piece of DNA from
  • a)
    A virus
  • b)
    A bacterium
  • c)
    A wild relative of cotton
  • d)
    An insect
Correct answer is option 'B'. Can you explain this answer?

Syed Saif answered
Bacillus killing the larvae of flour moths in Germany and published a description of the bacterium and its … Mycogen, another small biotechnology firm, instead cloned Bt genes and expressed them in an alternate host, Pseudomonas fluorescens, a bacterial species common in transgenic cotton.

A transgenic food crop which may help in solving the problem of night blindness in developing countries is
  • a)
    Bt soybean
  • b)
    Flavrsavr tomatoes
  • c)
    Golden rice
  • d)
    Starlink maize
Correct answer is option 'C'. Can you explain this answer?

Chirag Ghosh answered
Golden Rice as a solution to night blindness

Golden Rice is a genetically modified rice variety that has been developed to help combat vitamin A deficiency, which can lead to night blindness and other health problems in developing countries. The rice is genetically modified to produce beta-carotene, a precursor to vitamin A, which is not naturally found in traditional rice varieties.

How does Golden Rice work?

Golden Rice is genetically engineered to produce beta-carotene in the endosperm of the rice grain, which is the part of the plant that is eaten. Beta-carotene is a pigment that gives fruits and vegetables their orange or yellow color, and it is also a precursor to vitamin A. When humans consume beta-carotene, it is converted to vitamin A in the body.

Why is Golden Rice important?

Vitamin A deficiency is a serious health problem in many developing countries, particularly in Southeast Asia and Africa. According to the World Health Organization, an estimated 250 million preschool children are vitamin A deficient, and it is estimated that between 250,000 and 500,000 malnourished children become blind every year due to vitamin A deficiency. Golden Rice is one potential solution to this problem, as it provides a source of beta-carotene that can be converted to vitamin A in the body.

Conclusion

Golden Rice is a promising solution to the problem of vitamin A deficiency in developing countries, which can lead to night blindness and other health problems. While there is still some controversy surrounding genetically modified crops, Golden Rice has the potential to make a significant impact on the health and well-being of millions of people around the world.

A radioactively labeled Probe can be detected by
  • a)
    Autoradiography
  • b)
    UV rays
  • c)
    ELISA
  • d)
    PCR
Correct answer is option 'A'. Can you explain this answer?

Priyanka Iyer answered
Radioactively labeled probe can be detected by autoradiography. A single stranded DNA or RNA tagged with a radioactive molecule is allowed to hybridise to its complementary DNA in a clone of cells followed by detecting using autoradiography.

Read the given statements and select the correct option.
Statement 1: Transgenic mouse is termed as 'super mouse' because it is twice big in size than the normal mouse.
Statement 2: In 'super mouse', the gene for human growth factor has been introduced and expressed.
  • a)
    Both statements 1 and 2 are correct and statement 2 is correct explanation of statement 1 
  • b)
    Statement 1 is correct but statement 2 is incorrect but statement 2 is not the correct explanation of statement 1
  • c)
    Statement 1 is incorrect but statement 2 is correct
  • d)
    Both statements 1 and 2 are incorrect
Correct answer is option 'A'. Can you explain this answer?

Priya Menon answered
Statement 1 is incorrect but statement 2 is correct
Transgenic mice are called "super mice" because they have been genetically modified to express certain traits or genes that are not found in normal mice. The term "super mouse" does not refer to the size of the mouse, but rather to the fact that it has been genetically modified. In statement 2, it is correct that In 'super mouse', the gene for human growth factor has been introduced and expressed. This is one of the reasons why transgenic mice are often used in scientific research, as they can be used to model human diseases or to study the effects of certain drugs or therapies.

Insulin earlier was extracted from
  • a)
    Fishes
  • b)
    Turkeys
  • c)
    whales
  • d)
    Slaughtered cattle and pigs
Correct answer is option 'D'. Can you explain this answer?

Harshitha Dey answered
Earlier time insulin required for controlling blood sugar was obtained from slaughtered cattle and pigs. This insulin caused allergy in diabetic patients.

First gene therapy was done for
  • a)
    Down’s syndrome
  • b)
    Alkaptonuria
  • c)
    ADA deficiency
  • d)
    Cystic fibrosis
Correct answer is option 'C'. Can you explain this answer?

Shounak Nair answered
First gene therapy was done in 1990 to a 4 year old girl with a ADA deficiency. ADA enzyme is essential for proper functioning of immune system.

Which variety of rice was patented by a U.S. company even though the highest number of varieties of this rice is found in India?
  • a)
    Sharbati Sonara
  • b)
    Co-667
  • c)
    Basmati
  • d)
    Lerma Roja
Correct answer is option 'C'. Can you explain this answer?

Priya Sen answered
Introduction
The patenting of rice varieties has sparked significant debate, particularly when a U.S. company patents a variety predominantly found in countries like India. Among these varieties, Basmati rice stands out as a notable example.
What is Basmati Rice?
- Basmati rice is known for its unique aroma, long grains, and fluffy texture.
- It has been cultivated in the Indian subcontinent for centuries and is a staple in various traditional dishes.
Patenting Issues
- In the late 1990s, a U.S. company, RiceTec, patented hybrid varieties that they claimed to be Basmati.
- This patent raised concerns among Indian farmers and stakeholders who argued that Basmati is a traditional variety that has been cultivated for generations in India and Pakistan.
Consequences of Patenting
- The patent led to legal battles over the rights to the name "Basmati."
- It highlighted the complexities of intellectual property rights in agriculture, especially concerning indigenous crops.
Conclusion
The case of Basmati rice serves as a significant example of the intersection between traditional agricultural practices and modern patent laws. Despite the patenting attempts, the highest number of Basmati rice varieties continues to be cultivated in India, emphasizing the importance of preserving indigenous agricultural heritage while navigating the challenges posed by globalization and intellectual property rights.

The first transgenic cow was
  • a)
    Rosie
  • b)
    Dolly
  • c)
    Siana
  • d)
    Verra
Correct answer is option 'A'. Can you explain this answer?

Vaibhav Basu answered
The first transgenic cow is Rosie which produced human protein riched milk having alpha-lactalbumin, which is more balanced than conventional cow’s milk.

Cry I Ab controls
  • a)
    Stem Borer
  • b)
    Corn Borer
  • c)
    Cotton Bollworm
  • d)
    Root pest
Correct answer is option 'B'. Can you explain this answer?

Harshitha Dey answered
Specific Bt toxin gene is isolated from Bacillus thuringiensis and incorporated into several crop plants. The protein encoded by gene CryI Ab controls corn borer.

Gene can therapy can be referred to as
  • a)
    pre - clinical testing for inherited diseases in newborns
  • b)
    treatment of diseases caused by genetic defect
  • c)
    genetic engineering using rDNA technology
  • d)
    cancer treatment using in vitro cultured stem cells
Correct answer is option 'B'. Can you explain this answer?

Geetika Shah answered
Gene therapy is a collection of methods, that allows correction of gene defect that has been diagnosed in a child/embryo. Correcting of a genetic defect involves delivery of a normal gene into the individual or embryo to take over the function of and compensate for the non-functional gene.

Rules of conduct that may be used to regulate our activities in relation to the biological world is called
  • a)
    bioethics
  • b)
    biowar
  • c)
    biopatent
  • d)
    biopiracy
Correct answer is option 'A'. Can you explain this answer?

Suresh Iyer answered
Bioethics may be viewed as the set of standards that may be used to regulate various activities based on their effects on the biological world. This is because biotechnology has aroused social as well as political concerns, which have ranged from biotechnology being unnatural to detrimental to biodiversity.

Sheep Dolly was genetically similar to
  • a)
    The mother from which nucleated fertilized egg was taken
  • b)
    The mother from which the nucleus of udder cell was taken
  • c)
    The surrogate mother
  • d)
    Both surrogate mother and nuclear donor mother
Correct answer is option 'B'. Can you explain this answer?

Mira Joshi answered
Dolly, the sheep, was cloned in 1997, Ian wilmur and his colleagues at the Roslin Research Scotland, took cells from ewe (mother sheep’s udder) They stored these udder cells in low nutrient culture. This stopped the starved cells from dividing switched of their active genes. One compete udder cell with its nucleus was selected, because its nucleus carries the mother's genetic information in the mean time, an unfertilised egg cell was taken from a different ewe. Its nucleus was removed leaving an empty cell containing all the essential machinery to produce an embryo. They then fused udder cell nucleus with the empty egg cell by electrical stimulation. The altered egg was cultured for six days.
Out of many resulting embryos, one was implanted in the uterus of the surrogate mother, where it grew into a lamb, So Dolly was born, genetically identical to mother sheep because her first cell nucleus came from mother’s cell. Dolly's birth proved that adult cells could acquire the cloning potential of embryonic cells.

Early detection of a disease is possible by
  • a)
    PCR
  • b)
    Gene therapy
  • c)
    Recombinant DNA technology and ELISA
  • d)
    both (a) and (c)
Correct answer is option 'D'. Can you explain this answer?

Raghav Bansal answered
Using conventional methods of diagnosis (serum and urine analysis, etc.) early detection is not possible. Recombinant DNA technology, Polymerase Chain Reaction (PCR) and Enzyme Linked Immuno-sorbent Assay (ELISA) are some of the techniques that serve the purpose of early diagnosis.

A GM crop is _______
  • a)
    mature crop
  • b)
    infected crop
  • c)
    raised to green manure
  • d)
    transgenic crop
Correct answer is option 'D'. Can you explain this answer?

Lead Academy answered
GM stands for Genetically Modified.
  • A GM crop is a transgenic crop in which the genes of the desired crop are changed by manipulations using various methods.
  • This method gives a high yield without waste of crops.

A monopoly granted to a person who has either invented a new and useful article, made improvement in an existing article or invented a new process of making an article is called
  • a)
    biopiracy
  • b)
    bioethics
  • c)
    patent
  • d)
    genetic modification
Correct answer is option 'C'. Can you explain this answer?

Anjali Sharma answered
A patent is the right granted by government to an inventor to prevent others from commercial use of his invention. When patents are granted for biological entities and for products derived from them, these patents are called biopatents. Primarily, industrialised countries, like U.S.A., Japan and members of European Union, are awarding biopatents.

An important objective of biotechnology in the area of agriculture is ________
  • a)
    to decrease seed number
  • b)
    to produce pest-resistant varieties of plants
  • c)
    to increase phosphorous, nitrogen production
  • d)
    to reduce the number of plants
Correct answer is option 'B'. Can you explain this answer?

EduRev NEET answered
An important objective of biotechnology in the area of agriculture is to produce pest-resistant varieties of plants.
  • This will help in less wasting of crops with high yield.
  • It will also give more products in less time and space.

Which of the following companies started selling humulin in the year 1983?
  • a)
    Eli Lilly
  • b)
    Genetech
  • c)
    GEAC
  • d)
    None of these
Correct answer is option 'A'. Can you explain this answer?

Answer:

Eli Lilly is the company that started selling Humulin in the year 1983.

Explanation:

Humulin is a type of insulin used to treat diabetes, and it was the first genetically engineered drug to be approved by the US Food and Drug Administration (FDA). The development of Humulin was a joint effort between Eli Lilly and Genentech, but Eli Lilly was the company responsible for marketing and selling the drug.

The approval of Humulin in 1982 marked a significant milestone in the field of biotechnology and paved the way for the development of more genetically engineered drugs. Eli Lilly has since continued to be a major player in the diabetes market, developing and marketing other insulin products such as Humalog and Basaglar.

In summary, Eli Lilly started selling Humulin in the year 1983, making it the first genetically engineered drug to be marketed and sold to the public.

Which of the following has been covered under the broad patent category?
  • a)
    Triticum
  • b)
    Oryza
  • c)
    Pissum sativum
  • d)
    Brassica
Correct answer is option 'B'. Can you explain this answer?

Preethi Saha answered
Broad patent category refers to patents that cover a large category of organisms or genetic material. In this case, the correct answer is option 'B' - Oryza.

Explanation:

- Triticum: It is a genus of plants that includes wheat species. However, it is not a broad patent category as it refers to a specific genus of plants.
- Pissum sativum: It is the scientific name for peas. Similar to Triticum, it refers to a specific plant species and not a broad patent category.
- Brassica: It is a genus of plants that includes several species such as mustard, cabbage, and broccoli. However, it is not a broad patent category as it refers to a specific genus of plants.
- Oryza: It is a genus of plants that includes several species of rice. Oryza has been covered under the broad patent category as it refers to a large category of organisms.

In conclusion, the correct answer is option 'B' - Oryza as it has been covered under the broad patent category.

Which of the following statements regarding gene therapy is/are correct?
  • a)
    It is an application of biotechnology, in which a defective gene is manipulated by introduction of a normal, healthy and functional gene
  • b)
    The genetic disorders that are being investigated for gene therapy, range from sickle-cell anaemia to severe combined immuno-deficiency (SCID)
  • c)
    The first clinical gene therapy was given in 1990 to a 4-year old girl with adenosine deaminase (ADA) deficiency
  • d)
    All of these
Correct answer is option 'D'. Can you explain this answer?

Mira Joshi answered
Gene therapy is a technique for correcting defective genes responsible for disease development. Under intensive investigation are, disease ranging from the rare genetic diseases caused by single mutations like sickle cell anaemia to killer diseases such as server combined immuno-deficiency (SCID). The first clinical gene therapy was given in 1990 to a 4-years old girl with adenosine deaminase (ADA) deficiency. This enzymes is very important for the immune system to function. ADA deficiency can lead to severe combined immuno-deficiency (SCID).

Read the given statements and select the correct options
Statement 1: In recombinant DNA technology, human genes are often transferred into bacteria (prokaryotes) or yeast (eukaryote).
Statement 2: Both bacteria and yeast multiply very fast to form huge population which expresses the desired gene.
  • a)
    Both statements 1 and 2 are correct and statement 2 is the correct explanation of statement 1.
  • b)
    Both Both statements 1 and 2 are correct but statement 2 is not the correct explanation of statement 1.
  • c)
    Statement 1 is correct and statement 2 is incorrect.
  • d)
    Both statements 1 and 2 are incorrect.
Correct answer is option 'A'. Can you explain this answer?

EduRev NEET answered
Recombinant DNA is a form of DNA whose nucleotide sequence has undergone alteration as a result of incorporation of exchange with another DNA strand. In recombinant DNA technology, human genes are often transferred into bacteria or yeast because both bacteria and yeast multiply very fast to form huge population, which expresses the desired gene. By doing so, one can observe the phenotypes of the gene more quickly and in a larger scale than by studying the same traist in humans.

For which of the disease transgenic models do not exists now days?
  • a)
    Tuberculosis
  • b)
    Cancer
  • c)
    Cystic fibrosis
  • d)
    Alzheimer’s disease.
Correct answer is option 'A'. Can you explain this answer?

Priyanka Iyer answered
Transgenic model for treatment of disease is available today for cancer, cystic fibrosis, Alzheimer’s disease and rheumatoid arthritis disease.

The organisation which makes decisions regarding the validity of GM research and the safety of introducing GM-organisms for public services is
  • a)
    Genetic Engineering Approval Committee
  • b)
    Genome Environment Action Committee
  • c)
    Genetic Environment Approval Committee
  • d)
    Genetics and Ethical Issue Action Committee
Correct answer is option 'A'. Can you explain this answer?

Ajay Yadav answered
Genetic modification of organisms can have unpredictable results when such organisms are introduced into the ecosystem. Therefore, the Indian Government has set up organisations such as GEAC (Genetic Engineering Approval Committee), which will make decisions regarding the validity of GM research and the safety of introducing GM-organisms for public services.

Technique used to detect the DNA in a clone is
  • a)
    polymerase chain reaction
  • b)
    gel electrophoresis
  • c)
    chromatography
  • d)
    autoradiography
Correct answer is option 'D'. Can you explain this answer?

Jyoti Sengupta answered
A single stranded DNA or RNA joined with a radioactive molecule (probe) is allowed to hybridise to its complementary DNA in a clone of cells. It is followed by detection using autoradiography. The clone having the mutated gene will not appear on the photographic film, because the probe will not have the complementarity with the mutated gene. 

A doctor while operating on an HIV (+)ve patient accidentally cuts himself with a scalpel. Suspecting himself to have contracted the virus which test will he take to rule out/confirm his suspicion?
  • a)
    PCR
  • b)
    Routine urine examination
  • c)
    TLC
  • d)
    DLC
Correct answer is option 'A'. Can you explain this answer?

Anshu Banerjee answered
Test to confirm HIV infection

The doctor in this scenario needs to get tested to confirm if he has contracted HIV. The test that he needs to take is the Polymerase Chain Reaction (PCR) test.

Explanation

PCR is a highly accurate and sensitive test that detects the genetic material (RNA) of the virus in the blood. It can detect the virus even in the early stages of infection before antibodies are produced by the body. This makes it the most reliable test to confirm HIV infection, especially in cases where there is a risk of transmission through accidental exposure like in this scenario.

Other tests like routine urine examination, Total Leukocyte Count (TLC), and Differential Leukocyte Count (DLC) are not specific for HIV and cannot detect the virus. They may indicate an infection or inflammation in the body but cannot confirm HIV.

Therefore, if the doctor wants to rule out or confirm his suspicion of HIV infection, he should undergo PCR testing. Early detection and treatment can significantly improve the quality of life of people living with HIV and prevent the progression of the disease.

During the processing of proinsulin into the mature insulin
  • a)
    C -peptide is added to proinsulin
  • b)
    C-peptide is removed from proinsulin
  • c)
    B-peptide is added to proinsulin
  • d)
    B-peptide is removed from proinsulin
Correct answer is option 'B'. Can you explain this answer?

Bhavya Menon answered
Explanation:
During the processing of proinsulin into the mature insulin, the C-peptide is removed from proinsulin.

Proinsulin is a single chain polypeptide consisting of three regions: the N-terminal B-chain, the C-terminal A-chain, and a connecting peptide (C-peptide) in the middle. The connecting peptide or C-peptide is important because it connects the B-chain and A-chain of proinsulin.

During the processing of proinsulin, the C-peptide is cleaved enzymatically from the precursor molecule. This cleavage reaction is catalyzed by proteolytic enzymes called prohormone convertases (PCs) in the secretory granules of pancreatic beta-cells.

Once the C-peptide is removed, the remaining A and B chains of proinsulin are held together by disulfide bonds to form mature insulin. Mature insulin is then secreted into the bloodstream in response to increased glucose levels.

The removal of C-peptide is important for two reasons:

1. Regulation of insulin secretion: C-peptide is co-secreted with insulin in equimolar amounts, and its removal allows for more precise control of insulin secretion.

2. Diagnostic value: C-peptide levels can be measured in blood to assess insulin secretion and to differentiate between type 1 and type 2 diabetes. Since C-peptide has a longer half-life than insulin, measuring C-peptide levels can provide a more accurate assessment of insulin secretion over time.

In conclusion, during the processing of proinsulin into mature insulin, the C-peptide is enzymatically cleaved from the precursor molecule, leaving the A and B chains of insulin held together by disulfide bonds.

What might be an advantage of beginning gene therapy prior to birth?
  • a)
    This would give the body plenty of time to utilise the new genes
  • b)
    The body would not reject it as it has not yet recognised ‘self’
  • c)
    The cells being extremely young, are more receptive to gene therapy
  • d)
    Probably there is not any advantage
Correct answer is option 'B'. Can you explain this answer?

Juhi Sharma answered
The foreign genesc) It could potentially prevent the development of genetic disorders or diseases before they occurd) It could allow for more targeted and precise gene editing, as the cells and tissues are still developing and more malleablee) It could potentially eliminate the need for gene therapy later in life, as the corrected genes would be present from the beginningf) It could potentially improve the overall health and quality of life for individuals with genetic disorders or diseasesg) It could allow for the correction or modification of multiple genes simultaneously, addressing multiple issues at onceh) It could potentially prevent the transmission of genetic disorders or diseases to future generationsi) It could allow for early intervention and personalized treatment plans, leading to better outcomes and prognosis for individuals with genetic disorders or diseases.

The transgenic animals are those which have
  • a)
    Foreign RNA in all of its cells
  • b)
    Foreign DNA in some of its cells
  • c)
    Foreign DNA in all of its cells
  • d)
    Both (a) and (c)
Correct answer is option 'C'. Can you explain this answer?

Transgenic Animals

Transgenic animals are those animals that have been genetically modified by the insertion of foreign DNA into their genome. This technology is widely used in biomedical research and biotechnology to study gene function, produce medically important proteins, and develop new therapies.

Foreign DNA in all of its cells

The correct answer to the question is option C, which is "Foreign DNA in all of its cells." This means that transgenic animals have foreign DNA integrated into their genome in every cell of their body. This is usually accomplished by injecting foreign DNA into the pronucleus of a fertilized egg, which is then implanted into a surrogate mother.

Applications of Transgenic Animals

Transgenic animals have many applications in biomedical research and biotechnology, including:

1. Production of therapeutic proteins: Transgenic animals can be engineered to produce human proteins that are used in medicine, such as insulin, growth hormones, and clotting factors.

2. Disease modeling: Transgenic animals can be used to model human diseases, such as cancer, Alzheimer's disease, and cystic fibrosis.

3. Gene therapy: Transgenic animals can be used to test and develop gene therapy treatments for genetic diseases.

4. Agricultural applications: Transgenic animals can be engineered to have desirable traits, such as increased milk production or disease resistance.

5. Conservation: Transgenic animals can be used to save endangered species by introducing beneficial genes into their populations.

Conclusion

In conclusion, transgenic animals are those animals that have been genetically modified by the insertion of foreign DNA into their genome. This technology has many applications in biomedical research and biotechnology and has the potential to improve human health and conserve endangered species.

A human protein which is being obtained from transgenic animals and is used to treat emphysema is
  • a)
    alpha-lactalbumin
  • b)
    thyroxine
  • c)
    α−1- antitrypsin
  • d)
    insulin
Correct answer is option 'C'. Can you explain this answer?

Suresh Iyer answered
A human protein which is obtained from transgenic animals and is used to treat emphysema is α−1-antitrypsin. Transgenic animals that produce useful biological products can be created by the introduction of the portion of DNA which codes for particular product such as human proteins. 

Which Indian plants have either been patented or attempts have been made to patent them by western nations for their commercial use?
  • a)
    Basmati rice
  • b)
    Turmeric
  • c)
    Neem
  • d)
    All of these have been targeted
Correct answer is option 'D'. Can you explain this answer?

Hansa Sharma answered
India is a country rich in tradition and expertise in natural medicines, spices, food preparations, biological pesticides and divers agriculture. Through painting without consist, foreign companies have collared at least 22 plants for their beneficial derivatives. Patients have been taken out on plants such as black papper (Piper nigrum), basmati rice (Oryaza sativa) Indian mustard (Brassica Campestris). Pomegranate (Punica granatum), turmeric (Curcuma domestica) and neem (Azadirachta indica). US, Japanse and German companies are the principal patenting pirates.

Read the given statements and select the correct option
Statement 1: PCR technique is helpful in detecting bacterial and viral diseases even when symptoms of the disease are not yet visible
Statement 2: Very low concentrations of bacteria or viruses in human body can be detected by amplification of their nucleic acids using the PCR technique
  • a)
    Both statements 1 and 2 are correct and statement 2 is the correct explanation of statement 1.
  • b)
    Both Both statements 1 and 2 are correct but statement 2 is not the correct explanation of statement 1.
  • c)
    Statement 1 is correct and statement 2 is incorrect.
  • d)
    Both statements 1 and 2 are incorrect.
Correct answer is option 'A'. Can you explain this answer?

Arya Sen answered
Bb

The polymerase chain reaction (PCR) technique is a powerful tool that allows the amplification of specific DNA sequences. It is widely used in various fields, including medical diagnostics.

PCR can be used to detect bacterial and viral diseases, even when symptoms of the disease are not yet visible. This is because PCR can detect the presence of the pathogen's DNA or RNA in a sample, even at very low concentrations.

PCR works by using specific primers that bind to the target DNA sequence of interest. These primers are designed to be complementary to the pathogen's DNA or RNA, allowing them to selectively amplify the target sequence.

Once the target sequence is amplified, it can be detected using various methods, such as gel electrophoresis or fluorescence-based detection. The presence of the amplified DNA indicates the presence of the pathogen in the sample.

Therefore, statement 1 is correct. PCR technique is indeed helpful in detecting bacterial and viral diseases, even before symptoms of the disease are visible.

bb

One of the key advantages of PCR is its ability to detect very low concentrations of bacteria or viruses in the human body. This is because PCR has the ability to amplify the target DNA or RNA sequence, increasing its concentration.

Even if the concentration of the pathogen's DNA or RNA is extremely low, PCR can amplify it to levels that can be easily detected. This allows for the early detection of infections, even before the symptoms become apparent.

Therefore, statement 2 is also correct. Very low concentrations of bacteria or viruses in the human body can indeed be detected by amplification of their nucleic acids using the PCR technique.

b

In conclusion, both statement 1 and statement 2 are correct. The PCR technique is helpful in detecting bacterial and viral diseases, even when symptoms of the disease are not yet visible. Additionally, very low concentrations of bacteria or viruses in the human body can be detected by amplification of their nucleic acids using PCR. Statement 2 is the correct explanation of statement 1, as PCR's ability to amplify target DNA or RNA sequences allows for the detection of pathogens even at low concentrations.

Which step has been taken by Government of India to cater to the requirement of patent terms and other emergency provisions in this regard?
  • a)
    Biopiracy Act
  • b)
    Indian Patents Bill
  • c)
    ETI Act
  • d)
    Negotiable instruments Act
Correct answer is option 'B'. Can you explain this answer?

Dev Patel answered
The Indian Parliament has cleared the second amendment of the Indian Patents Bill. It takes into consideration unauthorised exploitation of bio-resources. It also involves patent terms, emergency provisions and research and development initiative.

Which of the following statements is incorrect about gene therapy in ADA deficiency?
  • a)
    Lymphocytes from patient's blood are taken out and cultured
  • b)
    A functional ADA−cDNA is introduced into these lymphocytes
  • c)
    Lymphocytes are then introduced in the body of patient
  • d)
    Patient does not require periodic infusion of genetically engineered lymphocytes
Correct answer is option 'D'. Can you explain this answer?

Lavanya Menon answered
As a first step towards gene therapy, lymphocytes from the blood of the patient are grown in a culture outside the body. A functional ADA−cDNA (using a retroviral vector) is then introduced into these lymphocytes, which are subsequently returned to the body of the patient. However, as these cells are not immortal, the patient requires periodic infusion of such genetically engineered lymphocytes.

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