NEET Exam  >  NEET Questions  >  Technique used to detect the DNA in a clone i... Start Learning for Free
Technique used to detect the DNA in a clone is
  • a)
    polymerase chain reaction
  • b)
    gel electrophoresis
  • c)
    chromatography
  • d)
    autoradiography
Correct answer is option 'D'. Can you explain this answer?
Verified Answer
Technique used to detect the DNA in a clone isa)polymerase chain react...
A single stranded DNA or RNA joined with a radioactive molecule (probe) is allowed to hybridise to its complementary DNA in a clone of cells. It is followed by detection using autoradiography. The clone having the mutated gene will not appear on the photographic film, because the probe will not have the complementarity with the mutated gene. 
View all questions of this test
Most Upvoted Answer
Technique used to detect the DNA in a clone isa)polymerase chain react...
Detecting DNA in a clone using autoradiography

Autoradiography is a technique used to detect the presence and distribution of radioactive molecules in a sample. It is commonly used in molecular biology and genetics to detect and visualize DNA or RNA fragments.

Principle of autoradiography

Autoradiography utilizes the ability of radioactive molecules to emit radiation, such as beta particles or gamma rays. The sample under investigation is exposed to a radioactive molecule, such as a radioactive isotope-labeled DNA probe. The radioactive emissions from the probe are then captured on a photographic film or through the use of a phosphor screen.

Procedure

The detection of DNA in a clone using autoradiography involves several steps:

1. Labeling the DNA probe: The DNA probe is typically labeled with a radioactive isotope, such as ^32P or ^35S. This is achieved by incorporating the radioactive isotope into the DNA molecule during its synthesis.

2. Hybridization: The labeled DNA probe is then allowed to hybridize with the target DNA in the clone. The DNA probe is designed to be complementary to a specific sequence within the target DNA. If the target DNA is present in the clone, it will bind to the labeled probe.

3. Exposure: The hybridized sample is then exposed to a photographic film or a phosphor screen. The radioactive emissions from the labeled DNA probe expose the film or activate the phosphor screen at the locations where the probe has bound to the target DNA.

4. Development: The photographic film is developed or the phosphor screen is scanned to visualize the radioactive emissions. The areas of the film or screen that have been exposed to the radioactivity appear as dark spots or bands, indicating the presence of the target DNA in the clone.

Advantages of autoradiography

- High sensitivity: Autoradiography is a highly sensitive technique that can detect very small amounts of DNA in a sample.
- Specificity: The use of a labeled DNA probe allows for the specific detection of the target DNA sequence within the clone.
- Visualization: Autoradiography provides a visual representation of the distribution of DNA in the clone, allowing for easy interpretation and analysis.

In conclusion, autoradiography is a technique used to detect DNA in a clone by utilizing a radioactive isotope-labeled DNA probe. The radioactive emissions from the labeled probe are captured on a photographic film or phosphor screen, providing a visual representation of the presence and distribution of DNA in the clone.
Attention NEET Students!
To make sure you are not studying endlessly, EduRev has designed NEET study material, with Structured Courses, Videos, & Test Series. Plus get personalized analysis, doubt solving and improvement plans to achieve a great score in NEET.
Explore Courses for NEET exam

Top Courses for NEET

Technique used to detect the DNA in a clone isa)polymerase chain reactionb)gel electrophoresisc)chromatographyd)autoradiographyCorrect answer is option 'D'. Can you explain this answer?
Question Description
Technique used to detect the DNA in a clone isa)polymerase chain reactionb)gel electrophoresisc)chromatographyd)autoradiographyCorrect answer is option 'D'. Can you explain this answer? for NEET 2024 is part of NEET preparation. The Question and answers have been prepared according to the NEET exam syllabus. Information about Technique used to detect the DNA in a clone isa)polymerase chain reactionb)gel electrophoresisc)chromatographyd)autoradiographyCorrect answer is option 'D'. Can you explain this answer? covers all topics & solutions for NEET 2024 Exam. Find important definitions, questions, meanings, examples, exercises and tests below for Technique used to detect the DNA in a clone isa)polymerase chain reactionb)gel electrophoresisc)chromatographyd)autoradiographyCorrect answer is option 'D'. Can you explain this answer?.
Solutions for Technique used to detect the DNA in a clone isa)polymerase chain reactionb)gel electrophoresisc)chromatographyd)autoradiographyCorrect answer is option 'D'. Can you explain this answer? in English & in Hindi are available as part of our courses for NEET. Download more important topics, notes, lectures and mock test series for NEET Exam by signing up for free.
Here you can find the meaning of Technique used to detect the DNA in a clone isa)polymerase chain reactionb)gel electrophoresisc)chromatographyd)autoradiographyCorrect answer is option 'D'. Can you explain this answer? defined & explained in the simplest way possible. Besides giving the explanation of Technique used to detect the DNA in a clone isa)polymerase chain reactionb)gel electrophoresisc)chromatographyd)autoradiographyCorrect answer is option 'D'. Can you explain this answer?, a detailed solution for Technique used to detect the DNA in a clone isa)polymerase chain reactionb)gel electrophoresisc)chromatographyd)autoradiographyCorrect answer is option 'D'. Can you explain this answer? has been provided alongside types of Technique used to detect the DNA in a clone isa)polymerase chain reactionb)gel electrophoresisc)chromatographyd)autoradiographyCorrect answer is option 'D'. Can you explain this answer? theory, EduRev gives you an ample number of questions to practice Technique used to detect the DNA in a clone isa)polymerase chain reactionb)gel electrophoresisc)chromatographyd)autoradiographyCorrect answer is option 'D'. Can you explain this answer? tests, examples and also practice NEET tests.
Explore Courses for NEET exam

Top Courses for NEET

Explore Courses
Signup for Free!
Signup to see your scores go up within 7 days! Learn & Practice with 1000+ FREE Notes, Videos & Tests.
10M+ students study on EduRev