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All questions of Biotechnology & its Applications for EmSAT Achieve Exam

 A technology which has found immense use in solving cases of disputed parentage is
  • a)
    Monoclonal antibody production
  • b)
    DNA finger printing
  • c)
    Polymerase chain reaction
  • d)
    Recombinant DNA technology
Correct answer is option 'B'. Can you explain this answer?

Mohammad Karim answered
Option B - for exapmple for identifying the true father DNA samples of child, mother and possible fathers are taken and their DNA fingerprints are obtained. the print of child DNA Match to the prints of biological parents

Conversion of sugar into alcohol during fermentation is due to the direct action of
  • a)
    Temperature
  • b)
    Microorganisms
  • c)
    Zymase
  • d)
    Concentration of sugar solution
Correct answer is option 'C'. Can you explain this answer?

Zymase is the enzyme which changes the simple sugars, dextrose and fructose into carbon dioxide gas and alcohol at 370C, and several esters which result in producing the unique aroma flavor and taste of bread.
Sugar or starch is used as the carbohydrate source. Zymase is the enzyme found in yeast and some bacteria.

* that why option """c""" is correct

The American company which is involved in the manufacturing of Humulin is
  • a)
    Elixir
  • b)
    Ranbaxy
  • c)
    Prohealth
  • d)
    Eli Lily
Correct answer is option 'D'. Can you explain this answer?

Harshitha Dey answered
In 1983, the American company Eli Lily prepared two DNA sequences corresponding to A and B and introduced into plasmid of E.coli to produce insulin chain.

 Cultivation of Bt cotton has been much in the news. The prefix Bt means
  • a)
    “Barium treated” cotton seeds
  • b)
    “Bigger thread” variety of cotton with better tensile strength
  • c)
    Carrying an endotoxin gene from Bacillus thuringiensis
  • d)
    Produced by “biotechnology” using restriction enzymes and ligases
Correct answer is option 'C'. Can you explain this answer?

Rajeev Saxena answered
Bt-cotton, Bt-tobacco, Bt-tomato etc., are transgenic plants having Bt-2 gene encoding Bt toxin (e.g., Thurioside). Bt-toxin gene has been isolated from a bacteria Bacillus thuringiensis there for called Bt. (i.e., Bacillus thuringiensis). These plants are resistant for more than 140 species of insects including common cabbage worm, tent caterpillars, bag worms, canker worms, gypsy worm etc.

The term ‘Humulin’ is used for
  • a)
    Hydrolytic enzyme
  • b)
    Isoenzyme
  • c)
    Human insulin
  • d)
    Antibiotic
Correct answer is option 'C'. Can you explain this answer?

Rohan Singh answered
Humulin is human insulin used for treating diabetes. Prior to its development, diabetics used insulin isolated from pig and cow pancreases. Developed by Genentech, the first American biotechnology company, Humulin was licensed to Eli Lilly and became the first marketable product created through recombinant DNA technology. Its licensing by the FDA in October 1982 also made it the first recombinant pharmaceutical approved for use in the United States.

Which of the following is not applicable for a biopatent?
  • a)
    Formation of new products
  • b)
    Unearthing the DNA sequences
  • c)
    Discovery of cell lines
  • d)
    Using the existing technology to produce new products
Correct answer is option 'C'. Can you explain this answer?

Geetika Shah answered
A biological patent is a patent on an invention in the field of biology that by law allows the patent holder to exclude others from making, using, selling, or importing the protected invention for a limited period of time. Hence, the correct option is c.

Hybridoma technology has been successfully used in
  • a)
    Production of alcohol in bulk
  • b)
    Synthesis of monoclonal antibodies
  • c)
    Synthesis of haemoglobin
  • d)
    Production of somatic hybrids
Correct answer is option 'B'. Can you explain this answer?

Rajat Kapoor answered
Hybridoma technology has contributed to virtually all areas of biology and medicine and set the scene for important advances in cell biology and immunodiagnostics. Blood transfusion and transfusion medicine have benefited significantly from development in hybridoma technology, and this has resulted in the generation of a wide range of monoclonal antibodies to human blood group antigens. These antibodies have also had an impact on diagnostic techniques such as enzyme-linked immunosorbent assay and flow cytometry as well as on the application of therapeutic agents in the clinical environment. However, rapid advances in the development of knockout and transgenic mice as well as in nucleic acid-based immunization may well lead hybridoma technology into a new era.

 Insect resistance transgenic cotton has been produced by inserting a piece of DNA from
  • a)
    A virus
  • b)
    A bacterium
  • c)
    A wild relative of cotton
  • d)
    An insect
Correct answer is option 'B'. Can you explain this answer?

Syed Saif answered
Bacillus killing the larvae of flour moths in Germany and published a description of the bacterium and its … Mycogen, another small biotechnology firm, instead cloned Bt genes and expressed them in an alternate host, Pseudomonas fluorescens, a bacterial species common in transgenic cotton.

The term antibiotic was coined by
  • a)
    Alexander Fleming
  • b)
    S.Waksman
  • c)
    Louis Pasteur
  • d)
    Edward Jenner
Correct answer is option 'B'. Can you explain this answer?

Sanjana Singh answered
Selman Waksman, the microbiologist who discovered streptomycin , first used the word 'Antibiotic' in medical in 1943.
So, the Correct answers is 'Waksman'.

The bacteria generally used for genetic engineering is                              
  • a)
    Agro bacterium
  • b)
    Bacillus
  • c)
    Pseudomonas
  • d)
    Clostridium
Correct answer is option 'A'. Can you explain this answer?

Ananya Singh answered
The two most important bacterias are E.coli (which is used for commercial production) and Agrobacterium i.e. agrobacterium tumifaciens.
Agrobacterium has T. DNA which is transferred in host cells. That's why this bacteria is called as natural genetic engineer which is generally used for genetic engineering.

Which one of the microorganism is used for production of citric acid in industries ?
  • a)
    Lactobacillus bulgaricus
  • b)
    Penicillium citrinum
  • c)
    Aspergillus niger
  • d)
    Rhizopus nigricans
Correct answer is option 'C'. Can you explain this answer?

Vivek Patel answered
Microorganisms can produce citric acid. The fungus Aspergillus Niger is most commonly used for industrial production of citric acid. The other organisms (although less important) include A. clavatus, A. wentii, Penicillium luteum, Candida catenula, C. guilliermondii and Corynebacterium sp.

A transgenic food crop which may help in solving the problem of night blindness in developing countries is
  • a)
    Bt soybean
  • b)
    Flavrsavr tomatoes
  • c)
    Golden rice
  • d)
    Starlink maize
Correct answer is option 'C'. Can you explain this answer?

Chirag Ghosh answered
Golden Rice as a solution to night blindness

Golden Rice is a genetically modified rice variety that has been developed to help combat vitamin A deficiency, which can lead to night blindness and other health problems in developing countries. The rice is genetically modified to produce beta-carotene, a precursor to vitamin A, which is not naturally found in traditional rice varieties.

How does Golden Rice work?

Golden Rice is genetically engineered to produce beta-carotene in the endosperm of the rice grain, which is the part of the plant that is eaten. Beta-carotene is a pigment that gives fruits and vegetables their orange or yellow color, and it is also a precursor to vitamin A. When humans consume beta-carotene, it is converted to vitamin A in the body.

Why is Golden Rice important?

Vitamin A deficiency is a serious health problem in many developing countries, particularly in Southeast Asia and Africa. According to the World Health Organization, an estimated 250 million preschool children are vitamin A deficient, and it is estimated that between 250,000 and 500,000 malnourished children become blind every year due to vitamin A deficiency. Golden Rice is one potential solution to this problem, as it provides a source of beta-carotene that can be converted to vitamin A in the body.

Conclusion

Golden Rice is a promising solution to the problem of vitamin A deficiency in developing countries, which can lead to night blindness and other health problems. While there is still some controversy surrounding genetically modified crops, Golden Rice has the potential to make a significant impact on the health and well-being of millions of people around the world.

During the formation of bread it becomes porous due to release of CO2 by the action of
  • a)
    Yeast
  • b)
    Bacteria
  • c)
    Virus
  • d)
    Protozoans
Correct answer is option 'A'. Can you explain this answer?

Vishal Kumar answered
Many breads are leavened by yeast. The yeast most commonly used for leavening bread is Saccharomyces cerevisiae, the same species used for brewing alcoholic beverages. This yeast ferments some of the carbohydrates in the flour, including any sugar, producing carbon dioxide. Commercial bakers often leaven their dough with commercially produced baker's yeast. Baker's yeast has the advantage of producing uniform, quick, and reliable results, because it is obtained from a pure culture. Many artisan bakers produce their own yeast with a growth culture. If kept in the right conditions, it provides leavening for many years.

The microorganism grown on molasses and sold as a food flavouring substance is
  • a)
    Saccharomyces
  • b)
    Rhizopus
  • c)
    Acetobacter
  • d)
    Lactobacillus
Correct answer is option 'A'. Can you explain this answer?

Gaurav Kumar answered
The strain of Saccharomyces cerevisiae fungus is commonly known as baker's yeast. The organism is widely used in baking industry. It has the ability to produce desirable flavor. The organisms are added to the dough of bread and cakes and allowed to perform fermentation. The fermentation is brought by converting the fermentable sugars to alcohol and carbon dioxide. The organisms are also cultured or grown on molasses. They bring about the anaerobic fermentation and this helps in providing flavor to the substance. 
Thus, the correct answer is option A. 

A radioactively labeled Probe can be detected by
  • a)
    Autoradiography
  • b)
    UV rays
  • c)
    ELISA
  • d)
    PCR
Correct answer is option 'A'. Can you explain this answer?

Priyanka Iyer answered
Radioactively labeled probe can be detected by autoradiography. A single stranded DNA or RNA tagged with a radioactive molecule is allowed to hybridise to its complementary DNA in a clone of cells followed by detecting using autoradiography.

Human insulin is being commercially produced from a transgenic species of
  • a)
    Mycobacterium
  • b)
    Rhizobium
  • c)
    Saccharomyces
  • d)
    Escherichia
Correct answer is option 'D'. Can you explain this answer?

Raza Great answered
Human insulin is produced from genetically engineered E.coli. By using genetic engineering or recombinant DNA technology, insulin-producing genes from human beings have been transferred into E.coli. bacteria, which produced insulin called 'humulin' for clinical use. This type of insulin was produced commercially by an American pharmaceutical firm Eli Lily on 5th July, 1983

A free living nitrogen-fixing cyanobacterium which can also from symbiotic association with the water fern Azolla is           
  • a)
    Anabaena
  • b)
    Tolypothrix
  • c)
    Chlorella
  • d)
    Nostoc
Correct answer is option 'A'. Can you explain this answer?

Anabaena is a free-living nitrogen fixing cyanobacterium which can form symbiotic association with water fern Azolla. The alga Tolypothrix belongs to class-Cyanophyceae. Some forms of Chlorella form symbiotic association in lichens and in certain invertebrates such as Hydra, Paramecium and sponges. Some species of Nostoc have been reported to fix atmospheric nitrogen and tend to maintain fertility of paddy fields.

Which one of the following is not true about antibiotics
  • a)
    First antibiotic was discovered by Alexander Fleming
  • b)
    The term 'antibiotic' was coined by S. Waksman in 1942.
  • c)
    Some persons can be allergic to a particular antibiotic
  • d)
    Each antibiotic is effective only against one particular kind of germ.
Correct answer is option 'D'. Can you explain this answer?

Rocky Handsome answered
• The antibiotic is the chemical substance produced by the microorganisms that can kill or inhibit the growth of the other microorganisms. There are two types of antibiotics on the basis of their action.

-》 The broad-spectrum antibiotic refers to an antibiotic that acts against a wide range of disease-causing bacteria. A broad-spectrum antibiotic act against both Gram-positive and Gram-negative bacteria, in contrast to a narrow-spectrum antibiotic, which is effective against specific families of bacteria. An example of a commonly used broad-spectrum antibiotic is ampicillin.

•Thus, the correct answer is option D.

The new strain of bacteria produced by biotechnology in alcohol industry is
  • a)
    Escherichia coli
  • b)
    Saccharomyces cerevisiae
  • c)
    Bacillus subtilis
  • d)
    Pseudomonas putida
Correct answer is option 'D'. Can you explain this answer?

Suresh Iyer answered
Pseudomonas putida F1 (Bacteria 6.2 Mb) is a versatile environmental isolate that is capable of growth on several aromatic hydrocarbons, including benzene, toluene, ethylbenzene and p -cymene. Its broad substrate toluene dioxygenase has been widely utilized in biocatalytic syntheses of chiral chemicals, as well as in the metabolism and detoxification of TCE, and in the production of indigo from indole. P. putida F1 is known to be chemotactic to aromatic hydrocarbons and chlorinated aliphatic compounds and has the potential for use in bioremediation applications. 
 

Propionic-bacteria is an important source of
  • a)
    Vitamin-C
  • b)
    Cobalamin
  • c)
    Sugars
  • d)
    Proteins
Correct answer is option 'B'. Can you explain this answer?

Biomass of the bacteria from the Propionibacterium genus constitutes sources of vitamins from the B group, including B 12, trehalose and numerous bacteriocins. These bacteria are also capable of synthesizing organic acids such as propionic acid and acetic acid.
Cobalamin also called as vitamin B12, it is an important vitamin for the normal formation of red blood cells and for the health of the nerve tissues. Undetected and untreated vitamin B12 deficiency can lead to anemia and permanent nerve and brain damage.

Cheese and yoghurt are products of
  • a)
    Pasteurisation
  • b)
    Distillation
  • c)
    Dehydration
  • d)
    Fermentation
Correct answer is option 'D'. Can you explain this answer?

Swati Verma answered
Fermented milk is the collective name for products such as yoghurt, ymer, kefir, cultured buttermilk, filmjolk (Scandinavian sour milk), cultured cream and koumiss (a product based on mares’ milk). The generic name of fermented milk is derived from the fact that the milk for the product is inoculated with a starter culture which converts part of the lactose to lactic acid.

Insulin earlier was extracted from
  • a)
    Fishes
  • b)
    Turkeys
  • c)
    whales
  • d)
    Slaughtered cattle and pigs
Correct answer is option 'D'. Can you explain this answer?

Harshitha Dey answered
Earlier time insulin required for controlling blood sugar was obtained from slaughtered cattle and pigs. This insulin caused allergy in diabetic patients.

First gene therapy was done for
  • a)
    Down’s syndrome
  • b)
    Alkaptonuria
  • c)
    ADA deficiency
  • d)
    Cystic fibrosis
Correct answer is option 'C'. Can you explain this answer?

Shounak Nair answered
First gene therapy was done in 1990 to a 4 year old girl with a ADA deficiency. ADA enzyme is essential for proper functioning of immune system.

Escherichia coli is used as an indicator organism to determine pollution of water with
  • a)
    Heavy metals
  • b)
    Faecal matter
  • c)
    Industrial effluents
  • d)
    Pollen of aquatic plant
Correct answer is option 'B'. Can you explain this answer?

Escherichia coli is the most common indicator of water pollution. It naturally occurs in the intestines of human beings and animals. They are commonly found in sewage and if E.coli are detected in water then it indicates fecal contamination. So if E.coli are detected in drinking water it indicates a serious health risk and that water should not be used for drinking. Hence, the correct answer is option 'B'.

First artificially synthesised hormone is
  • a)
    Secretin
  • b)
    Insulin
  • c)
    Glucagon
  • d)
    Renin
Correct answer is option 'B'. Can you explain this answer?

Raza Great answered
First artificially synthesized hormone is human insulin or humulin. It was prepared by inserting the insulin gene into a suitable vector, the E. coli bacterial cell, to produce an insulin that is chemically identical to its naturally produced counterpart. This has been achieved using recombinant DNA technology

The first transgenic cow was
  • a)
    Rosie
  • b)
    Dolly
  • c)
    Siana
  • d)
    Verra
Correct answer is option 'A'. Can you explain this answer?

Vaibhav Basu answered
The first transgenic cow is Rosie which produced human protein riched milk having alpha-lactalbumin, which is more balanced than conventional cow’s milk.

Biogas is produced by anaerobic breakdown of biomass of agricultural waste by methanogenic bacteria. It is a
  • a)
    One step process                
  • b)
    Two step process
  • c)
    Three step process
  • d)
    Multi step process
Correct answer is option 'C'. Can you explain this answer?

Rajni Kokate answered
Answer c is correct.. the steps are as follows
1. the facultative anaerobic microbes degrade the complex polymers to simple monomers by enzymatic action.
2. 2nd stage , monomers are converted into organic acids by microbial action and finally converted to acetic acid.
3. in 3rd stage, acetic acid is oxidized into methane by activity of anaerobic methanogenic bacteria.

Formation of vinegar from alcohol is caused by
  • a)
    Bacillus subtilis
  • b)
    Clostridium
  • c)
    Acetobacter aceti
  • d)
    Azotobacter
Correct answer is option 'C'. Can you explain this answer?

Raza Great answered
Acetobacter is a genus of acetic acid bacteria characterized by the ability to convert ethanol to acetic acid in the presence of oxygen. Acetobacter is of particular importance commercially, because some species are used in the production of vinegar. It is economically important because it is used in the production of vinegar by converting the ethanol in wine into acetic acid

Maximum percentage of alcohol present in the product of yeast fermentation 
  • a)
    Rum
  • b)
    Gin
  • c)
    Brandy
  • d)
    Wine
Correct answer is option 'A'. Can you explain this answer?

Pankaj Datta answered
Alcoholic beverages have three categories: wine, beer and distilled liquors. Beer is produced by the fermentation of grains which produces only about 3-6% alcohol. Wine is produced by the fermentation of fruit and produces wine with an alcohol content of 6-14%. A distilled beverage, spirit, liquor or hard liquor is an alcoholic beverage produced by distillation of a mixture produced from alcoholic fermentation. This process purifies it and removes diluting components like water, for the purpose of increasing its proportion of alcohol content. Brandy a spirit, is distinct as a drink from wine (due to distillation), and has a 35% of alcohol content. Other examples of common distilled beverages include vodka, gin, tequila, rum, whisky. 
Rum - 37.5%-80%, Brandy - 35%-60% (usually 40%), Vodka - 35%-50%. Thus, the correct answer is option A.

Saccharomyces cerevisiae is used in the formation of                             
  • a)
    Ethanol
  • b)
    Methanol
  • c)
    Acetic acid
  • d)
    Antibiotics
Correct answer is option 'A'. Can you explain this answer?

Rocky Handsome answered
¥¥Saccharomyces cerevisiae is famously known for its role in food production. It is the critical component in the fermentation process that converts sugar into alcohol, an ingredient shared in beer, wine and distilled beverages. It is also used in the baking process as a leavening agent; yeast releasing gas into their environment results in the spongy-like texture of breads and cakes. Because of its role in fermentation, humans have known about and used Saccharomyces cerevisiae for a long time. 

---)) In a process called as fermentation, yeast feeds off sugars from their substrate and convert it to ethanol,

Cry I Ab controls
  • a)
    Stem Borer
  • b)
    Corn Borer
  • c)
    Cotton Bollworm
  • d)
    Root pest
Correct answer is option 'B'. Can you explain this answer?

Harshitha Dey answered
Specific Bt toxin gene is isolated from Bacillus thuringiensis and incorporated into several crop plants. The protein encoded by gene CryI Ab controls corn borer.

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