The ploidy of the cells of microspore tetrad is “n” means haploid. Here, as the formation of pollen includes many stages and a very common confusion everyone has about ploidy of cell (stages) can be resolved by a simple rule. Trying to explain below-
Stamen ( microsporophyll), have two segments, anther and filament.
Anther is commonly bilobed, dithecous and tetrasporangiate. It has 4 layers or walls. Named- epidermis, endothecium, middle layers, tapetum.
Inside the layers there is sporogenous tissue which is made up of sporogenous cells. Now each cell surround itself with callose wall (callose- a sugar).
Now till here each and every segment, stage cell, part etc etc …. all was diploid (2n) from very bebeginning - seed , root, tissue even the smallest cell of plant was diploid. But the only cell which will undergo meiosis cell division in the entire plant body is….
P.M.C. pollen mother cell and the important thing PMC itself is diploid. This will undergo meiosis and produce 4 haploid cells with ploidy n (of each).
They will be surrounded with callose wall and being together forming a tetrad in premature state.
Then the pollen will get nutrition and enzymes from the tapetum. It deforms the tertad.
Matured anther dehices with the help of endothecium from the line of dehicence stomium.
Then, pollen grains ( male gametophyte ) comes out and wait for the factor through which they can reach their destination. Soon…