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Proteins give blue colour with
  • a)
    Benedict's reagent
  • b)
    iodine solution
  • c)
    ninhydrin reagent
  • d)
    biuret
Correct answer is option 'C'. Can you explain this answer?
Most Upvoted Answer
Proteins give blue colour witha)Benedict's reagentb)iodine solutio...
The reaction of amino acids with ninhydrin is colorimetric .
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Community Answer
Proteins give blue colour witha)Benedict's reagentb)iodine solutio...
Understanding Protein Detection with Ninhydrin Reagent
Ninhydrin is a chemical reagent commonly used in biochemistry to detect amino acids and proteins. The reaction of ninhydrin with amino acids results in a color change, making it an effective tool for protein analysis.
How Ninhydrin Works
- Ninhydrin reacts with amino acids, which are the building blocks of proteins.
- Upon reaction, ninhydrin loses a molecule of carbon dioxide and water, resulting in the formation of a colored complex.
- This complex is typically blue or purple in color, depending on the concentration of amino acids present.
Comparison with Other Reagents
- Benedict’s Reagent: Primarily used to test for reducing sugars, not proteins. It gives a red or orange color with reducing sugars, not blue.
- Iodine Solution: This reagent is used to test for starch. It produces a blue-black color in the presence of starch, not proteins.
- Biuret Test: While the biuret test also detects proteins, it produces a violet or purple color, not blue, when proteins are present.
Conclusion
The correct answer to the question is option 'C' (Ninhydrin reagent) because it specifically gives a blue color in the presence of amino acids and proteins. This distinct color change makes ninhydrin a valuable tool for identifying and quantifying proteins in various biological samples.
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Proteins give blue colour witha)Benedict's reagentb)iodine solutionc)ninhydrin reagentd)biuretCorrect answer is option 'C'. Can you explain this answer?
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