cDNA is formed bya)DNA dependent DNA polymeraseb)RNA dependent DNA polymerasec)DNA dependent RNA polymerased)DNA ligaseCorrect answer is option 'B'. Can you explain this answer?

Question

Answer

C Dna is formed by reverse transcriptase this enzyme convert RNA into DNA ,since it works on RNA and its product is DNA so it is also known as RNA dependent DNA polymerase because it depend on RNA to form DNA.

Answer

Reverse transcription
ie formation of DNA from RNA
So RNA dependent dna polymerase

Answer

Formation of cDNA by RNA dependent DNA polymerase:
Reverse Transcription Process:
- cDNA (complementary DNA) is formed by the enzyme reverse transcriptase, which is an RNA dependent DNA polymerase.
- In the process of reverse transcription, the enzyme reverse transcriptase uses an RNA template to synthesize a complementary DNA strand.
- This process allows the conversion of RNA into DNA, which is essential for various molecular biology techniques.
Role of Reverse Transcriptase:
- Reverse transcriptase is a key enzyme in the formation of cDNA. It catalyzes the synthesis of DNA from an RNA template.
- This enzyme is commonly used in research to study gene expression, create cDNA libraries, and perform various genetic manipulations.
Applications of cDNA:
- cDNA is widely used in molecular biology research for gene cloning, expression analysis, and functional genomics studies.
- It is particularly valuable in studying eukaryotic genes, as it represents the coding sequences of mRNA molecules.
Summary:
- cDNA is formed by reverse transcriptase, an RNA dependent DNA polymerase, through the process of reverse transcription.
- This enzyme plays a crucial role in converting RNA into DNA, allowing for the study of gene expression and genetic manipulation.

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