Both are correct... If both the strands take part in transcription it may result in Gene silencing

Introduction:
In transcription, one of the two DNA strands serves as a template for the synthesis of RNA. This process involves the synthesis of a complementary RNA molecule using ribonucleotide triphosphates (rNTPs) and an RNA polymerase enzyme. The template strand, also known as the antisense strand or non-coding strand, provides the sequence information for RNA synthesis.

Assertion: Antisense strand, having 3'-5' polarity takes part in transcription.

Reason: Non-template strand having 5'-3' polarity does not take part in transcription.

Explanation:
Transcription is a highly regulated process that occurs in the nucleus of eukaryotic cells and in the cytoplasm of prokaryotic cells. It involves several steps, including initiation, elongation, and termination. The DNA template strand is used to synthesize an RNA molecule with a complementary sequence, except that thymine (T) is replaced by uracil (U) in RNA.

Template Strand:
The template strand, also known as the antisense strand or non-coding strand, is the DNA strand that is used as a template for RNA synthesis. It is called the antisense strand because its sequence is complementary to the RNA molecule that is transcribed. The template strand has a polarity of 3'-5', meaning that the DNA polymerase adds nucleotides in the 5' to 3' direction.

Antisense Strand in Transcription:
During transcription, the RNA polymerase enzyme binds to the DNA double helix and separates the two strands. The template strand is used as a guide to synthesize a complementary RNA molecule. The RNA polymerase moves along the template strand in the 3'-5' direction, adding rNTPs to the growing RNA chain in a 5'-3' direction. The resulting RNA molecule has the same sequence as the coding strand, except that thymine is replaced by uracil.

Non-Template Strand:
The non-template strand, also known as the coding strand or sense strand, has the same sequence as the RNA molecule, except that it contains thymine instead of uracil. However, the non-template strand is not directly involved in transcription. It is called the coding strand because its sequence corresponds to the protein-coding region of the DNA.

Conclusion:
In conclusion, the antisense strand or template strand with a 3'-5' polarity is actively involved in transcription. It provides the sequence information for RNA synthesis, and the RNA polymerase enzyme uses it as a template to synthesize a complementary RNA molecule. On the other hand, the non-template strand with a 5'-3' polarity does not directly participate in transcription but has the same sequence as the resulting RNA molecule, except for the presence of thymine instead of uracil.