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In agarose gel electrophoresis, DNA molecules are separated on the basis of their:
- a)Charge only
- b)Charge to size ratio
- c)both a and b
- d)Size only
Correct answer is option 'D'. Can you explain this answer?
Most Upvoted Answer
In agarose gel electrophoresis, DNA molecules are separated on the bas...
The smaller the size the farther it moves hence depend only on the size
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Community Answer
In agarose gel electrophoresis, DNA molecules are separated on the bas...
Agarose gel electrophoresis is a commonly used technique to separate DNA molecules based on their size. The process involves the use of a gel matrix made of agarose, which is placed in an electrophoresis chamber. The DNA sample is loaded onto the gel and an electric current is applied, causing the DNA molecules to move through the gel matrix.
Charge and Size
The separation of DNA molecules in agarose gel electrophoresis is primarily based on their size. The smaller DNA molecules move more quickly through the gel matrix than larger molecules, resulting in a separation of the DNA fragments based on size. The charge of the DNA molecules also plays a role in the process, as DNA molecules are negatively charged and will be attracted to the positively charged electrode.
Charge to Size Ratio
While the size of the DNA molecule is the primary factor in its separation, the charge to size ratio also plays a role. DNA molecules that are similar in size but have different charge to size ratios may be separated based on their charge. For example, a smaller DNA molecule with a higher charge to size ratio may move more quickly through the gel matrix than a larger molecule with a lower charge to size ratio.
Conclusion
In conclusion, agarose gel electrophoresis separates DNA molecules primarily based on their size, with the smaller molecules moving more quickly through the gel matrix. While charge to size ratio also plays a role in the separation, size is the primary factor.
Charge and Size
The separation of DNA molecules in agarose gel electrophoresis is primarily based on their size. The smaller DNA molecules move more quickly through the gel matrix than larger molecules, resulting in a separation of the DNA fragments based on size. The charge of the DNA molecules also plays a role in the process, as DNA molecules are negatively charged and will be attracted to the positively charged electrode.
Charge to Size Ratio
While the size of the DNA molecule is the primary factor in its separation, the charge to size ratio also plays a role. DNA molecules that are similar in size but have different charge to size ratios may be separated based on their charge. For example, a smaller DNA molecule with a higher charge to size ratio may move more quickly through the gel matrix than a larger molecule with a lower charge to size ratio.
Conclusion
In conclusion, agarose gel electrophoresis separates DNA molecules primarily based on their size, with the smaller molecules moving more quickly through the gel matrix. While charge to size ratio also plays a role in the separation, size is the primary factor.
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In agarose gel electrophoresis, DNA molecules are separated on the basis of their:a)Charge onlyb)Charge to size ratioc)both a and bd)Size onlyCorrect answer is option 'D'. Can you explain this answer? for NEET 2025 is part of NEET preparation. The Question and answers have been prepared according to the NEET exam syllabus. Information about In agarose gel electrophoresis, DNA molecules are separated on the basis of their:a)Charge onlyb)Charge to size ratioc)both a and bd)Size onlyCorrect answer is option 'D'. Can you explain this answer? covers all topics & solutions for NEET 2025 Exam. Find important definitions, questions, meanings, examples, exercises and tests below for In agarose gel electrophoresis, DNA molecules are separated on the basis of their:a)Charge onlyb)Charge to size ratioc)both a and bd)Size onlyCorrect answer is option 'D'. Can you explain this answer?.
In agarose gel electrophoresis, DNA molecules are separated on the basis of their:a)Charge onlyb)Charge to size ratioc)both a and bd)Size onlyCorrect answer is option 'D'. Can you explain this answer? for NEET 2025 is part of NEET preparation. The Question and answers have been prepared according to the NEET exam syllabus. Information about In agarose gel electrophoresis, DNA molecules are separated on the basis of their:a)Charge onlyb)Charge to size ratioc)both a and bd)Size onlyCorrect answer is option 'D'. Can you explain this answer? covers all topics & solutions for NEET 2025 Exam. Find important definitions, questions, meanings, examples, exercises and tests below for In agarose gel electrophoresis, DNA molecules are separated on the basis of their:a)Charge onlyb)Charge to size ratioc)both a and bd)Size onlyCorrect answer is option 'D'. Can you explain this answer?.
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