Dr. F went noted that if coleoptile tips were removed and placed on ag...
Significance of Dr. F's Experiment on Coleoptile Tips
Experiment Description: Dr. F observed that when coleoptile tips were removed and placed on agar for one hour, the agar would produce a bending when placed on one side of freshly cut coleoptile stumps.
Significance:
1. Isolation and Identification of Auxin: This experiment made it possible to isolate and identify auxin, a growth-promoting hormone found in plants. The agar produced the bending effect due to the presence of auxin in the coleoptile tips that had been placed on it.
2. Quantitative Determination of Growth-Promoting Substances: The experiment provided the basis for the quantitative determination of small amounts of growth-promoting substances. By measuring the degree of bending produced by the agar, scientists could determine the amount of auxin present in the coleoptile tips.
3. Support for the Hypothesis that IAA is Auxin: The experiment supported the hypothesis that indole-3-acetic acid (IAA) is the primary auxin in plants. This is because the agar produced the bending effect only when placed on the side of the coleoptile stump opposite to where the coleoptile tips had been placed. This demonstrated the polar movement of auxin, which is a characteristic of IAA.
4. Demonstration of Polar Movement of Auxin: The experiment demonstrated the polar movement of auxin, which is the movement of the hormone from the tip of the plant towards the base. The agar produced the bending effect only when placed on one side of the coleoptile stump, which indicated that the auxin had moved towards the other side.
In conclusion, Dr. F's experiment on coleoptile tips was significant in providing insights into the nature and function of auxin in plants. It enabled the isolation and identification of auxin, provided the basis for quantitative determination of growth-promoting substances, supported the hypothesis that IAA is the primary auxin, and demonstrated the polar movement of auxin in plants.
Dr. F went noted that if coleoptile tips were removed and placed on ag...
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