DNA precipitation out of a mixture of biomolecule can be achieved by treatment with?

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DNA precipitation out of  a mixture of biomolucule can be achieved by treatment with chilled ethonole.. this process is known as DNA spooling.

Answer

Answer

DNA precipitation out of a mixture of biomolecules can be achieved by treatment with:

DNA precipitation is a technique used to isolate and purify DNA from a mixture of biomolecules. The precipitation process involves the addition of certain reagents that cause the DNA to come out of solution and form a visible precipitate. There are several methods to achieve DNA precipitation, and one commonly used approach involves treatment with ethanol or isopropanol.

Procedure:

1. Sample Preparation: The first step in DNA precipitation is to prepare the sample containing the DNA of interest. This can be done by extracting DNA from cells or tissues using various methods such as phenol-chloroform extraction or commercial kits.

2. Precipitant Addition: Once the DNA-containing sample is prepared, a precipitant such as ethanol or isopropanol is added. These alcohols are used because they have a lower solubility in water than DNA, leading to the precipitation of DNA molecules.

3. Mixing: After the addition of the precipitant, the mixture is gently mixed to ensure proper distribution of the alcohol throughout the sample. This can be achieved by inverting the tube or by gentle vortexing.

4. Incubation: The mixture is then incubated at a low temperature, usually -20°C or -80°C. This allows the DNA to precipitate out of solution over time.

5. Centrifugation: After incubation, the mixture is subjected to centrifugation at a high speed. Centrifugation forces the DNA precipitate to settle at the bottom of the tube, while other biomolecules remain in the supernatant.

6. Washing: The DNA pellet obtained after centrifugation is washed with a cold ethanol or isopropanol solution to remove any impurities. This step helps to further purify the DNA.

7. Drying and Resuspension: The washed DNA pellet is then air-dried or briefly dried using a gentle stream of nitrogen gas. Finally, the DNA is resuspended in a suitable buffer for further downstream applications such as PCR, sequencing, or storage.

Conclusion:

DNA precipitation with ethanol or isopropanol is a widely used method for isolating and purifying DNA from a mixture of biomolecules. This technique takes advantage of the differential solubility of DNA and other biomolecules in alcohol solutions. By following the steps outlined above, DNA can be efficiently precipitated, separated from the other components of the sample, and obtained in a pure form for further analysis.

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