What bonds are cleaved when restriction endonuclease cuts DNA?

Question

Answer

Restriction endonuclease is also called restriction enzymes. Therefore, restriction enzymes cut DNA bonds between 3' OH of 1 nucleotide and 5' phosphate of the next one at the specific restriction site.

Answer

They cleave phosphodiester linkage between two nucleotides of DNA .they identify only specific sequence in the DNA.they produce sticky ends.so that they can replaced again in other alien DNA.

Answer

Restriction enzymes are the endonucleases which cleave the DNA at specific sites. These enzymes act on the phosphodiester bond which attaches two nucleotides in the polynucleotide chain. There are specific restriction sites at which the enzymes make blunt or staggered cuts to produce the fragments of the DNA.

So, the right answer is 'Phosphodiester bond'...

$$Hope it's help... $$

Answer

Restriction Endonuclease Cleavage of DNA Bonds
When a restriction endonuclease cuts DNA, it cleaves specific bonds within the DNA molecule.

Phosphodiester Bonds
- The primary bond that is cleaved by restriction endonucleases is the phosphodiester bond.
- Phosphodiester bonds connect the sugar-phosphate backbone of the DNA molecule.
- These bonds are cleaved by restriction endonucleases at specific recognition sites within the DNA sequence.

Hydrogen Bonds
- In addition to phosphodiester bonds, hydrogen bonds between complementary bases in the DNA double helix are also disrupted during the cleavage process.
- These hydrogen bonds hold the two strands of the DNA molecule together, and their disruption allows the DNA to be cut by the restriction endonuclease.

Specific Recognition Sites
- Restriction endonucleases recognize specific sequences of nucleotides within the DNA molecule.
- Once a restriction endonuclease binds to its specific recognition site, it cleaves the phosphodiester bonds in the DNA backbone.

Result of Cleavage
- The cleavage of DNA by restriction endonucleases results in the generation of DNA fragments with cohesive or blunt ends.
- These fragments can be further used in various molecular biology techniques such as cloning, PCR, and DNA sequencing.

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