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Read the following and answer the question given below :
Restriction endonuclease was isolated for the first time by W. Aber in 1962 in bacteria. Restriction endonucleases cut the DNA duplex at specific points therefore they are also called molecular scissors or biological scissors. Three types of restriction endonucleases are Type 1. Type ll and Type III but only Type II restriction endonucleases are used in recombinant DNA technology. Restriction endonuclease EcoR I recognises the base sequence GAATTC In DNA duplex and cut strands between G and A.
Q. Which type of restriction endonucleases is used most in genetic engineering?
  • a)
    Type I
  • b)
    Type II
  • c)
    Type III
  • d)
    Type IV
Correct answer is option 'B'. Can you explain this answer?
Verified Answer
Read the following and answer the question given below : Restriction ...
Type I and Type III are complex and have only a limited role in genetic engineering. Type II restriction endonucleases are used mostly as the cutting enzymes in gene cloning.
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Read the following and answer the question given below : Restriction ...
Introduction to Restriction Endonucleases
Restriction endonucleases, also known as molecular or biological scissors, are essential tools in genetic engineering. They are enzymes that cut DNA at specific sequences, allowing for precise manipulation of genetic material.
Types of Restriction Endonucleases
- Type I: These enzymes cut DNA at sites far from their recognition sequences and require ATP for activity.
- Type II: These are the most commonly used in genetic engineering. Type II restriction endonucleases cut DNA at specific sites within or adjacent to their recognition sequences, making them ideal for cloning and DNA manipulation.
- Type III: These cut DNA a short distance away from their recognition sites and also require ATP.
Why Type II is Preferred in Genetic Engineering
- Specificity: Type II restriction endonucleases recognize specific DNA sequences, which allows for targeted editing of genes. For example, EcoR I recognizes the sequence GAATTC and cuts between G and A.
- Simplicity: They do not require ATP for their activity, simplifying the experimental setup. This makes them easier to use in laboratory conditions.
- Versatility: Type II enzymes can be used in various applications such as cloning, gene mapping, and constructing recombinant DNA molecules.
Conclusion
Given these attributes, Type II restriction endonucleases are the most widely used in genetic engineering, ensuring precise and efficient manipulation of DNA. The correct answer to the question regarding the type of restriction endonucleases used most in genetic engineering is indeed option 'B', Type II.
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Read the following and answer the question given below : Restriction endonuclease was isolated for the first time by W. Aber in 1962 in bacteria. Restriction endonucleases cut the DNA duplex at specific points therefore they are also called molecular scissors or biological scissors. Three types of restriction endonucleases are Type 1. Type ll and Type III but only Type II restriction endonucleases are used in recombinant DNA technology. Restriction endonuclease EcoR I recognises the base sequence GAATTC In DNA duplex and cut strands between G and A.Q. Which type of restriction endonucleases is used most in genetic engineering?a)Type Ib)Type IIc)Type IIId)Type IVCorrect answer is option 'B'. Can you explain this answer?
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Read the following and answer the question given below : Restriction endonuclease was isolated for the first time by W. Aber in 1962 in bacteria. Restriction endonucleases cut the DNA duplex at specific points therefore they are also called molecular scissors or biological scissors. Three types of restriction endonucleases are Type 1. Type ll and Type III but only Type II restriction endonucleases are used in recombinant DNA technology. Restriction endonuclease EcoR I recognises the base sequence GAATTC In DNA duplex and cut strands between G and A.Q. Which type of restriction endonucleases is used most in genetic engineering?a)Type Ib)Type IIc)Type IIId)Type IVCorrect answer is option 'B'. Can you explain this answer? for NEET 2024 is part of NEET preparation. The Question and answers have been prepared according to the NEET exam syllabus. Information about Read the following and answer the question given below : Restriction endonuclease was isolated for the first time by W. Aber in 1962 in bacteria. Restriction endonucleases cut the DNA duplex at specific points therefore they are also called molecular scissors or biological scissors. Three types of restriction endonucleases are Type 1. Type ll and Type III but only Type II restriction endonucleases are used in recombinant DNA technology. Restriction endonuclease EcoR I recognises the base sequence GAATTC In DNA duplex and cut strands between G and A.Q. Which type of restriction endonucleases is used most in genetic engineering?a)Type Ib)Type IIc)Type IIId)Type IVCorrect answer is option 'B'. Can you explain this answer? covers all topics & solutions for NEET 2024 Exam. Find important definitions, questions, meanings, examples, exercises and tests below for Read the following and answer the question given below : Restriction endonuclease was isolated for the first time by W. Aber in 1962 in bacteria. Restriction endonucleases cut the DNA duplex at specific points therefore they are also called molecular scissors or biological scissors. Three types of restriction endonucleases are Type 1. Type ll and Type III but only Type II restriction endonucleases are used in recombinant DNA technology. Restriction endonuclease EcoR I recognises the base sequence GAATTC In DNA duplex and cut strands between G and A.Q. Which type of restriction endonucleases is used most in genetic engineering?a)Type Ib)Type IIc)Type IIId)Type IVCorrect answer is option 'B'. Can you explain this answer?.
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