Read the following and answer the question given below : Restriction ...
Type I and Type III are complex and have only a limited role in genetic engineering. Type II restriction endonucleases are used mostly as the cutting enzymes in gene cloning.
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Read the following and answer the question given below : Restriction ...
Introduction to Restriction Endonucleases
Restriction endonucleases, also known as molecular or biological scissors, are essential tools in genetic engineering. They are enzymes that cut DNA at specific sequences, allowing for precise manipulation of genetic material.
Types of Restriction Endonucleases
- Type I: These enzymes cut DNA at sites far from their recognition sequences and require ATP for activity.
- Type II: These are the most commonly used in genetic engineering. Type II restriction endonucleases cut DNA at specific sites within or adjacent to their recognition sequences, making them ideal for cloning and DNA manipulation.
- Type III: These cut DNA a short distance away from their recognition sites and also require ATP.
Why Type II is Preferred in Genetic Engineering
- Specificity: Type II restriction endonucleases recognize specific DNA sequences, which allows for targeted editing of genes. For example, EcoR I recognizes the sequence GAATTC and cuts between G and A.
- Simplicity: They do not require ATP for their activity, simplifying the experimental setup. This makes them easier to use in laboratory conditions.
- Versatility: Type II enzymes can be used in various applications such as cloning, gene mapping, and constructing recombinant DNA molecules.
Conclusion
Given these attributes, Type II restriction endonucleases are the most widely used in genetic engineering, ensuring precise and efficient manipulation of DNA. The correct answer to the question regarding the type of restriction endonucleases used most in genetic engineering is indeed option 'B', Type II.
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