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Read the following and answer the question given below :
Restriction endonuclease was isolated for the first time by W. Aber in 1962 in bacteria. Restriction endonucleases cut the DNA duplex at specific points therefore they are also called molecular scissors or biological scissors. Three types of restriction endonucleases are Type 1. Type ll and Type III but only Type II restriction endonucleases are used in recombinant DNA technology. Restriction endonuclease EcoR I recognises the base sequence GAATTC In DNA duplex and cut strands between G and A.
Q. Only type II restriction enzymes are used in gene manipulation because:
- a)ATP is not required for cleaving
- b)it consists of three different subunits
- c)it makes cleavage or cut in both the strands of DNA molecule
- d)both (A) and (C)
Correct answer is option 'D'. Can you explain this answer?
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Read the following and answer the question given below : Restriction ...
Type II enzymes are simpler and don't require ATP as an energy source, unlike Type I and it makes cleavage or cut in both the strands of DNA molecule.
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Read the following and answer the question given below : Restriction ...
Answer:
Introduction:
Restriction endonucleases are enzymes that cut DNA at specific recognition sequences. They are also known as molecular scissors or biological scissors because they cleave the DNA duplex at specific points. There are three types of restriction endonucleases: Type I, Type II, and Type III. However, only Type II restriction endonucleases are commonly used in recombinant DNA technology.
Reasons for using Type II restriction enzymes in gene manipulation:
The main reasons for using Type II restriction enzymes in gene manipulation are:
1. ATP is not required for cleaving:
Type II restriction enzymes do not require ATP (adenosine triphosphate) to cleave the DNA duplex. ATP is an energy molecule that is needed for many cellular processes. The absence of ATP requirement in Type II restriction enzymes simplifies the cleavage process and reduces the energy demand.
2. Cleavage in both strands of DNA molecule:
Type II restriction enzymes make cleavage or cut in both strands of the DNA molecule. This is important in gene manipulation because it allows for precise and controlled DNA cleavage. The specific recognition sequence of the Type II restriction enzyme determines the location of the cleavage site.
Examples:
One example of a Type II restriction enzyme is EcoR I. It recognizes the base sequence GAATTC in the DNA duplex and cuts the strands between the G and A. This specific cleavage site allows for the insertion of foreign DNA fragments at the cut site, which is a fundamental step in recombinant DNA technology.
Conclusion:
Type II restriction enzymes are widely used in gene manipulation due to their ability to cleave DNA without requiring ATP and their ability to make cuts in both strands of the DNA molecule. These enzymes play a crucial role in recombinant DNA technology by facilitating the insertion of foreign DNA fragments at specific locations in the DNA sequence.
Introduction:
Restriction endonucleases are enzymes that cut DNA at specific recognition sequences. They are also known as molecular scissors or biological scissors because they cleave the DNA duplex at specific points. There are three types of restriction endonucleases: Type I, Type II, and Type III. However, only Type II restriction endonucleases are commonly used in recombinant DNA technology.
Reasons for using Type II restriction enzymes in gene manipulation:
The main reasons for using Type II restriction enzymes in gene manipulation are:
1. ATP is not required for cleaving:
Type II restriction enzymes do not require ATP (adenosine triphosphate) to cleave the DNA duplex. ATP is an energy molecule that is needed for many cellular processes. The absence of ATP requirement in Type II restriction enzymes simplifies the cleavage process and reduces the energy demand.
2. Cleavage in both strands of DNA molecule:
Type II restriction enzymes make cleavage or cut in both strands of the DNA molecule. This is important in gene manipulation because it allows for precise and controlled DNA cleavage. The specific recognition sequence of the Type II restriction enzyme determines the location of the cleavage site.
Examples:
One example of a Type II restriction enzyme is EcoR I. It recognizes the base sequence GAATTC in the DNA duplex and cuts the strands between the G and A. This specific cleavage site allows for the insertion of foreign DNA fragments at the cut site, which is a fundamental step in recombinant DNA technology.
Conclusion:
Type II restriction enzymes are widely used in gene manipulation due to their ability to cleave DNA without requiring ATP and their ability to make cuts in both strands of the DNA molecule. These enzymes play a crucial role in recombinant DNA technology by facilitating the insertion of foreign DNA fragments at specific locations in the DNA sequence.
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Read the following and answer the question given below : Restriction endonuclease was isolated for the first time by W. Aber in 1962 in bacteria. Restriction endonucleases cut the DNA duplex at specific points therefore they are also called molecular scissors or biological scissors. Three types of restriction endonucleases are Type 1. Type ll and Type III but only Type II restriction endonucleases are used in recombinant DNA technology. Restriction endonuclease EcoR I recognises the base sequence GAATTC In DNA duplex and cut strands between G and A.Q. Only type II restriction enzymes are used in gene manipulation because:a)ATP is not required for cleavingb)it consists of three different subunitsc)it makes cleavage or cut in both the strands of DNA moleculed)both (A) and (C)Correct answer is option 'D'. Can you explain this answer?
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Read the following and answer the question given below : Restriction endonuclease was isolated for the first time by W. Aber in 1962 in bacteria. Restriction endonucleases cut the DNA duplex at specific points therefore they are also called molecular scissors or biological scissors. Three types of restriction endonucleases are Type 1. Type ll and Type III but only Type II restriction endonucleases are used in recombinant DNA technology. Restriction endonuclease EcoR I recognises the base sequence GAATTC In DNA duplex and cut strands between G and A.Q. Only type II restriction enzymes are used in gene manipulation because:a)ATP is not required for cleavingb)it consists of three different subunitsc)it makes cleavage or cut in both the strands of DNA moleculed)both (A) and (C)Correct answer is option 'D'. Can you explain this answer? for NEET 2024 is part of NEET preparation. The Question and answers have been prepared according to the NEET exam syllabus. Information about Read the following and answer the question given below : Restriction endonuclease was isolated for the first time by W. Aber in 1962 in bacteria. Restriction endonucleases cut the DNA duplex at specific points therefore they are also called molecular scissors or biological scissors. Three types of restriction endonucleases are Type 1. Type ll and Type III but only Type II restriction endonucleases are used in recombinant DNA technology. Restriction endonuclease EcoR I recognises the base sequence GAATTC In DNA duplex and cut strands between G and A.Q. Only type II restriction enzymes are used in gene manipulation because:a)ATP is not required for cleavingb)it consists of three different subunitsc)it makes cleavage or cut in both the strands of DNA moleculed)both (A) and (C)Correct answer is option 'D'. Can you explain this answer? covers all topics & solutions for NEET 2024 Exam. Find important definitions, questions, meanings, examples, exercises and tests below for Read the following and answer the question given below : Restriction endonuclease was isolated for the first time by W. Aber in 1962 in bacteria. Restriction endonucleases cut the DNA duplex at specific points therefore they are also called molecular scissors or biological scissors. Three types of restriction endonucleases are Type 1. Type ll and Type III but only Type II restriction endonucleases are used in recombinant DNA technology. Restriction endonuclease EcoR I recognises the base sequence GAATTC In DNA duplex and cut strands between G and A.Q. Only type II restriction enzymes are used in gene manipulation because:a)ATP is not required for cleavingb)it consists of three different subunitsc)it makes cleavage or cut in both the strands of DNA moleculed)both (A) and (C)Correct answer is option 'D'. Can you explain this answer?.
Read the following and answer the question given below : Restriction endonuclease was isolated for the first time by W. Aber in 1962 in bacteria. Restriction endonucleases cut the DNA duplex at specific points therefore they are also called molecular scissors or biological scissors. Three types of restriction endonucleases are Type 1. Type ll and Type III but only Type II restriction endonucleases are used in recombinant DNA technology. Restriction endonuclease EcoR I recognises the base sequence GAATTC In DNA duplex and cut strands between G and A.Q. Only type II restriction enzymes are used in gene manipulation because:a)ATP is not required for cleavingb)it consists of three different subunitsc)it makes cleavage or cut in both the strands of DNA moleculed)both (A) and (C)Correct answer is option 'D'. Can you explain this answer? for NEET 2024 is part of NEET preparation. The Question and answers have been prepared according to the NEET exam syllabus. Information about Read the following and answer the question given below : Restriction endonuclease was isolated for the first time by W. Aber in 1962 in bacteria. Restriction endonucleases cut the DNA duplex at specific points therefore they are also called molecular scissors or biological scissors. Three types of restriction endonucleases are Type 1. Type ll and Type III but only Type II restriction endonucleases are used in recombinant DNA technology. Restriction endonuclease EcoR I recognises the base sequence GAATTC In DNA duplex and cut strands between G and A.Q. Only type II restriction enzymes are used in gene manipulation because:a)ATP is not required for cleavingb)it consists of three different subunitsc)it makes cleavage or cut in both the strands of DNA moleculed)both (A) and (C)Correct answer is option 'D'. Can you explain this answer? covers all topics & solutions for NEET 2024 Exam. Find important definitions, questions, meanings, examples, exercises and tests below for Read the following and answer the question given below : Restriction endonuclease was isolated for the first time by W. Aber in 1962 in bacteria. Restriction endonucleases cut the DNA duplex at specific points therefore they are also called molecular scissors or biological scissors. Three types of restriction endonucleases are Type 1. Type ll and Type III but only Type II restriction endonucleases are used in recombinant DNA technology. Restriction endonuclease EcoR I recognises the base sequence GAATTC In DNA duplex and cut strands between G and A.Q. Only type II restriction enzymes are used in gene manipulation because:a)ATP is not required for cleavingb)it consists of three different subunitsc)it makes cleavage or cut in both the strands of DNA moleculed)both (A) and (C)Correct answer is option 'D'. Can you explain this answer?.
Solutions for Read the following and answer the question given below : Restriction endonuclease was isolated for the first time by W. Aber in 1962 in bacteria. Restriction endonucleases cut the DNA duplex at specific points therefore they are also called molecular scissors or biological scissors. Three types of restriction endonucleases are Type 1. Type ll and Type III but only Type II restriction endonucleases are used in recombinant DNA technology. Restriction endonuclease EcoR I recognises the base sequence GAATTC In DNA duplex and cut strands between G and A.Q. Only type II restriction enzymes are used in gene manipulation because:a)ATP is not required for cleavingb)it consists of three different subunitsc)it makes cleavage or cut in both the strands of DNA moleculed)both (A) and (C)Correct answer is option 'D'. Can you explain this answer? in English & in Hindi are available as part of our courses for NEET.
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Read the following and answer the question given below : Restriction endonuclease was isolated for the first time by W. Aber in 1962 in bacteria. Restriction endonucleases cut the DNA duplex at specific points therefore they are also called molecular scissors or biological scissors. Three types of restriction endonucleases are Type 1. Type ll and Type III but only Type II restriction endonucleases are used in recombinant DNA technology. Restriction endonuclease EcoR I recognises the base sequence GAATTC In DNA duplex and cut strands between G and A.Q. Only type II restriction enzymes are used in gene manipulation because:a)ATP is not required for cleavingb)it consists of three different subunitsc)it makes cleavage or cut in both the strands of DNA moleculed)both (A) and (C)Correct answer is option 'D'. Can you explain this answer?, a detailed solution for Read the following and answer the question given below : Restriction endonuclease was isolated for the first time by W. Aber in 1962 in bacteria. Restriction endonucleases cut the DNA duplex at specific points therefore they are also called molecular scissors or biological scissors. Three types of restriction endonucleases are Type 1. Type ll and Type III but only Type II restriction endonucleases are used in recombinant DNA technology. Restriction endonuclease EcoR I recognises the base sequence GAATTC In DNA duplex and cut strands between G and A.Q. Only type II restriction enzymes are used in gene manipulation because:a)ATP is not required for cleavingb)it consists of three different subunitsc)it makes cleavage or cut in both the strands of DNA moleculed)both (A) and (C)Correct answer is option 'D'. Can you explain this answer? has been provided alongside types of Read the following and answer the question given below : Restriction endonuclease was isolated for the first time by W. Aber in 1962 in bacteria. Restriction endonucleases cut the DNA duplex at specific points therefore they are also called molecular scissors or biological scissors. Three types of restriction endonucleases are Type 1. Type ll and Type III but only Type II restriction endonucleases are used in recombinant DNA technology. Restriction endonuclease EcoR I recognises the base sequence GAATTC In DNA duplex and cut strands between G and A.Q. Only type II restriction enzymes are used in gene manipulation because:a)ATP is not required for cleavingb)it consists of three different subunitsc)it makes cleavage or cut in both the strands of DNA moleculed)both (A) and (C)Correct answer is option 'D'. Can you explain this answer? theory, EduRev gives you an
ample number of questions to practice Read the following and answer the question given below : Restriction endonuclease was isolated for the first time by W. Aber in 1962 in bacteria. Restriction endonucleases cut the DNA duplex at specific points therefore they are also called molecular scissors or biological scissors. Three types of restriction endonucleases are Type 1. Type ll and Type III but only Type II restriction endonucleases are used in recombinant DNA technology. Restriction endonuclease EcoR I recognises the base sequence GAATTC In DNA duplex and cut strands between G and A.Q. Only type II restriction enzymes are used in gene manipulation because:a)ATP is not required for cleavingb)it consists of three different subunitsc)it makes cleavage or cut in both the strands of DNA moleculed)both (A) and (C)Correct answer is option 'D'. Can you explain this answer? tests, examples and also practice NEET tests.
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