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Which of the given statements is correct in the context of visualizing DNA molecules separated by agarose gel electrophoresis?
- a)Ethidium bromide stained DNA can be seen under exposure to UV light
- b)DNA can be seen without staining in visible light
- c)Ethidium bromide stained DNA can be seen in visible light
- d)DNA can be seen in visible light
Correct answer is option 'A'. Can you explain this answer?
Most Upvoted Answer
Which of the given statements is correct in the context of visualizing...
Visualizing DNA Molecules Separated by Agarose Gel Electrophoresis
Agarose gel electrophoresis is a widely used technique to separate DNA molecules based on their size. The separated DNA fragments can be visualized using different methods.
Ethidium Bromide Staining and UV Light
Ethidium bromide is a commonly used fluorescent dye that intercalates into the DNA double helix and emits fluorescence under UV light. When DNA is stained with ethidium bromide and exposed to UV light, the dye binds to the DNA and fluoresces, allowing the DNA bands to be visualized.
Correct Statement
Option 'A' is correct in the context of visualizing DNA molecules separated by agarose gel electrophoresis. Ethidium bromide stained DNA can be seen under exposure to UV light. The other options are incorrect because DNA cannot be seen without staining in visible light, and ethidium bromide stained DNA cannot be seen in visible light. However, DNA can be seen in visible light if it is stained with other dyes such as SYBR Green.
Significance
Visualization of DNA molecules separated by agarose gel electrophoresis is important in various molecular biology applications, including genomic DNA analysis, PCR product analysis, and DNA sequencing. Ethidium bromide staining and UV light visualization are the most commonly used methods due to their simplicity, sensitivity, and cost-effectiveness. However, the use of ethidium bromide has some safety concerns due to its mutagenic and carcinogenic properties. Therefore, alternative DNA stains such as SYBR Green and GelRed have been developed, which are safer and more environmentally friendly.
Agarose gel electrophoresis is a widely used technique to separate DNA molecules based on their size. The separated DNA fragments can be visualized using different methods.
Ethidium Bromide Staining and UV Light
Ethidium bromide is a commonly used fluorescent dye that intercalates into the DNA double helix and emits fluorescence under UV light. When DNA is stained with ethidium bromide and exposed to UV light, the dye binds to the DNA and fluoresces, allowing the DNA bands to be visualized.
Correct Statement
Option 'A' is correct in the context of visualizing DNA molecules separated by agarose gel electrophoresis. Ethidium bromide stained DNA can be seen under exposure to UV light. The other options are incorrect because DNA cannot be seen without staining in visible light, and ethidium bromide stained DNA cannot be seen in visible light. However, DNA can be seen in visible light if it is stained with other dyes such as SYBR Green.
Significance
Visualization of DNA molecules separated by agarose gel electrophoresis is important in various molecular biology applications, including genomic DNA analysis, PCR product analysis, and DNA sequencing. Ethidium bromide staining and UV light visualization are the most commonly used methods due to their simplicity, sensitivity, and cost-effectiveness. However, the use of ethidium bromide has some safety concerns due to its mutagenic and carcinogenic properties. Therefore, alternative DNA stains such as SYBR Green and GelRed have been developed, which are safer and more environmentally friendly.
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Which of the given statements is correct in the context of visualizing DNA molecules separated by agarose gel electrophoresis?a)Ethidium bromide stained DNA can be seen under exposure to UV lightb)DNA can be seen without staining in visible lightc)Ethidium bromide stained DNA can be seen in visible lightd)DNA can be seen in visible lightCorrect answer is option 'A'. Can you explain this answer? for NEET 2025 is part of NEET preparation. The Question and answers have been prepared according to the NEET exam syllabus. Information about Which of the given statements is correct in the context of visualizing DNA molecules separated by agarose gel electrophoresis?a)Ethidium bromide stained DNA can be seen under exposure to UV lightb)DNA can be seen without staining in visible lightc)Ethidium bromide stained DNA can be seen in visible lightd)DNA can be seen in visible lightCorrect answer is option 'A'. Can you explain this answer? covers all topics & solutions for NEET 2025 Exam. Find important definitions, questions, meanings, examples, exercises and tests below for Which of the given statements is correct in the context of visualizing DNA molecules separated by agarose gel electrophoresis?a)Ethidium bromide stained DNA can be seen under exposure to UV lightb)DNA can be seen without staining in visible lightc)Ethidium bromide stained DNA can be seen in visible lightd)DNA can be seen in visible lightCorrect answer is option 'A'. Can you explain this answer?.
Which of the given statements is correct in the context of visualizing DNA molecules separated by agarose gel electrophoresis?a)Ethidium bromide stained DNA can be seen under exposure to UV lightb)DNA can be seen without staining in visible lightc)Ethidium bromide stained DNA can be seen in visible lightd)DNA can be seen in visible lightCorrect answer is option 'A'. Can you explain this answer? for NEET 2025 is part of NEET preparation. The Question and answers have been prepared according to the NEET exam syllabus. Information about Which of the given statements is correct in the context of visualizing DNA molecules separated by agarose gel electrophoresis?a)Ethidium bromide stained DNA can be seen under exposure to UV lightb)DNA can be seen without staining in visible lightc)Ethidium bromide stained DNA can be seen in visible lightd)DNA can be seen in visible lightCorrect answer is option 'A'. Can you explain this answer? covers all topics & solutions for NEET 2025 Exam. Find important definitions, questions, meanings, examples, exercises and tests below for Which of the given statements is correct in the context of visualizing DNA molecules separated by agarose gel electrophoresis?a)Ethidium bromide stained DNA can be seen under exposure to UV lightb)DNA can be seen without staining in visible lightc)Ethidium bromide stained DNA can be seen in visible lightd)DNA can be seen in visible lightCorrect answer is option 'A'. Can you explain this answer?.
Solutions for Which of the given statements is correct in the context of visualizing DNA molecules separated by agarose gel electrophoresis?a)Ethidium bromide stained DNA can be seen under exposure to UV lightb)DNA can be seen without staining in visible lightc)Ethidium bromide stained DNA can be seen in visible lightd)DNA can be seen in visible lightCorrect answer is option 'A'. Can you explain this answer? in English & in Hindi are available as part of our courses for NEET.
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Which of the given statements is correct in the context of visualizing DNA molecules separated by agarose gel electrophoresis?a)Ethidium bromide stained DNA can be seen under exposure to UV lightb)DNA can be seen without staining in visible lightc)Ethidium bromide stained DNA can be seen in visible lightd)DNA can be seen in visible lightCorrect answer is option 'A'. Can you explain this answer?, a detailed solution for Which of the given statements is correct in the context of visualizing DNA molecules separated by agarose gel electrophoresis?a)Ethidium bromide stained DNA can be seen under exposure to UV lightb)DNA can be seen without staining in visible lightc)Ethidium bromide stained DNA can be seen in visible lightd)DNA can be seen in visible lightCorrect answer is option 'A'. Can you explain this answer? has been provided alongside types of Which of the given statements is correct in the context of visualizing DNA molecules separated by agarose gel electrophoresis?a)Ethidium bromide stained DNA can be seen under exposure to UV lightb)DNA can be seen without staining in visible lightc)Ethidium bromide stained DNA can be seen in visible lightd)DNA can be seen in visible lightCorrect answer is option 'A'. Can you explain this answer? theory, EduRev gives you an
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