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Plasmid DNA (0.5 μg) containing an ampicillin resistance marker was added to 200 μl of competent cells. The transformed competent cells were diluted 10,000 times, out of which, 50 μl was plated on agar plates containing ampicillin. A total of 35 colonies were obtained. The transformation efficiency is ______________× 106 cfu·μg-1.
Correct answer is '2.8'. Can you explain this answer?
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Plasmid DNA (0.5 μg) containing an ampicillin resistance marker was...
Plasmid DNA is a small, circular piece of DNA that is found in bacteria and some other organisms. It is separate from the organism's chromosomal DNA and can replicate independently. Plasmid DNA is commonly used in laboratory research and biotechnology applications.
The size of plasmid DNA can vary, but it is typically quite small, ranging from about 1 to 200 kilobase pairs (kb). A kb is a unit of measurement for DNA and represents about 1,000 base pairs, which are the building blocks of DNA.
When referring to the amount of plasmid DNA, it is often measured in micrograms (µg) or nanograms (ng). For example, 0.5 µg of plasmid DNA would be equivalent to 0.0005 milligrams (mg) or 0.0000005 grams (g).
In laboratory research, plasmid DNA is often used to introduce specific genes or genetic material into cells. It can be manipulated and modified in the lab, allowing scientists to study the function of specific genes or produce proteins of interest. Plasmid DNA is also commonly used in genetic engineering, where it can be used to produce genetically modified organisms or to create recombinant proteins.
The size of plasmid DNA can vary, but it is typically quite small, ranging from about 1 to 200 kilobase pairs (kb). A kb is a unit of measurement for DNA and represents about 1,000 base pairs, which are the building blocks of DNA.
When referring to the amount of plasmid DNA, it is often measured in micrograms (µg) or nanograms (ng). For example, 0.5 µg of plasmid DNA would be equivalent to 0.0005 milligrams (mg) or 0.0000005 grams (g).
In laboratory research, plasmid DNA is often used to introduce specific genes or genetic material into cells. It can be manipulated and modified in the lab, allowing scientists to study the function of specific genes or produce proteins of interest. Plasmid DNA is also commonly used in genetic engineering, where it can be used to produce genetically modified organisms or to create recombinant proteins.
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Plasmid DNA (0.5 μg) containing an ampicillin resistance marker was added to 200 μl of competent cells. The transformed competent cells were diluted 10,000 times, out of which, 50 μl was plated on agar plates containing ampicillin. A total of 35 colonies were obtained. The transformation efficiency is ______________× 106 cfu·μg-1.Correct answer is '2.8'. Can you explain this answer?
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Plasmid DNA (0.5 μg) containing an ampicillin resistance marker was added to 200 μl of competent cells. The transformed competent cells were diluted 10,000 times, out of which, 50 μl was plated on agar plates containing ampicillin. A total of 35 colonies were obtained. The transformation efficiency is ______________× 106 cfu·μg-1.Correct answer is '2.8'. Can you explain this answer? for GATE 2024 is part of GATE preparation. The Question and answers have been prepared according to the GATE exam syllabus. Information about Plasmid DNA (0.5 μg) containing an ampicillin resistance marker was added to 200 μl of competent cells. The transformed competent cells were diluted 10,000 times, out of which, 50 μl was plated on agar plates containing ampicillin. A total of 35 colonies were obtained. The transformation efficiency is ______________× 106 cfu·μg-1.Correct answer is '2.8'. Can you explain this answer? covers all topics & solutions for GATE 2024 Exam. Find important definitions, questions, meanings, examples, exercises and tests below for Plasmid DNA (0.5 μg) containing an ampicillin resistance marker was added to 200 μl of competent cells. The transformed competent cells were diluted 10,000 times, out of which, 50 μl was plated on agar plates containing ampicillin. A total of 35 colonies were obtained. The transformation efficiency is ______________× 106 cfu·μg-1.Correct answer is '2.8'. Can you explain this answer?.
Plasmid DNA (0.5 μg) containing an ampicillin resistance marker was added to 200 μl of competent cells. The transformed competent cells were diluted 10,000 times, out of which, 50 μl was plated on agar plates containing ampicillin. A total of 35 colonies were obtained. The transformation efficiency is ______________× 106 cfu·μg-1.Correct answer is '2.8'. Can you explain this answer? for GATE 2024 is part of GATE preparation. The Question and answers have been prepared according to the GATE exam syllabus. Information about Plasmid DNA (0.5 μg) containing an ampicillin resistance marker was added to 200 μl of competent cells. The transformed competent cells were diluted 10,000 times, out of which, 50 μl was plated on agar plates containing ampicillin. A total of 35 colonies were obtained. The transformation efficiency is ______________× 106 cfu·μg-1.Correct answer is '2.8'. Can you explain this answer? covers all topics & solutions for GATE 2024 Exam. Find important definitions, questions, meanings, examples, exercises and tests below for Plasmid DNA (0.5 μg) containing an ampicillin resistance marker was added to 200 μl of competent cells. The transformed competent cells were diluted 10,000 times, out of which, 50 μl was plated on agar plates containing ampicillin. A total of 35 colonies were obtained. The transformation efficiency is ______________× 106 cfu·μg-1.Correct answer is '2.8'. Can you explain this answer?.
Solutions for Plasmid DNA (0.5 μg) containing an ampicillin resistance marker was added to 200 μl of competent cells. The transformed competent cells were diluted 10,000 times, out of which, 50 μl was plated on agar plates containing ampicillin. A total of 35 colonies were obtained. The transformation efficiency is ______________× 106 cfu·μg-1.Correct answer is '2.8'. Can you explain this answer? in English & in Hindi are available as part of our courses for GATE.
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Plasmid DNA (0.5 μg) containing an ampicillin resistance marker was added to 200 μl of competent cells. The transformed competent cells were diluted 10,000 times, out of which, 50 μl was plated on agar plates containing ampicillin. A total of 35 colonies were obtained. The transformation efficiency is ______________× 106 cfu·μg-1.Correct answer is '2.8'. Can you explain this answer?, a detailed solution for Plasmid DNA (0.5 μg) containing an ampicillin resistance marker was added to 200 μl of competent cells. The transformed competent cells were diluted 10,000 times, out of which, 50 μl was plated on agar plates containing ampicillin. A total of 35 colonies were obtained. The transformation efficiency is ______________× 106 cfu·μg-1.Correct answer is '2.8'. Can you explain this answer? has been provided alongside types of Plasmid DNA (0.5 μg) containing an ampicillin resistance marker was added to 200 μl of competent cells. The transformed competent cells were diluted 10,000 times, out of which, 50 μl was plated on agar plates containing ampicillin. A total of 35 colonies were obtained. The transformation efficiency is ______________× 106 cfu·μg-1.Correct answer is '2.8'. Can you explain this answer? theory, EduRev gives you an
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