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A major breakthrough in the studies of cells came with the development of electron microscope because?
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A major breakthrough in the studies of cells came with the development...
Because the resolution power of electron microscope is much higher than that of the light microscope. Electron microscope uses a beam of electron which is a wavelength much longer than that of photons.
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A major breakthrough in the studies of cells came with the development...
Importance of Electron Microscope in Cell Studies
The development of the electron microscope was a major breakthrough in the studies of cells because of the following reasons:
- Higher Resolution: Electron microscopes use a beam of electrons instead of light to visualize samples, allowing for much higher resolution images compared to light microscopes. This enables scientists to observe cellular structures in much greater detail.
- Visualizing Small Structures: The high resolution of electron microscopes allows researchers to visualize smaller structures within cells, such as organelles like mitochondria, Golgi apparatus, and endoplasmic reticulum, which are not visible with light microscopes.
- Understanding Cell Functions: By being able to see cellular structures at a higher resolution, scientists can better understand the functions of different organelles and how they contribute to the overall function of the cell. This has led to significant advancements in cell biology research.
- Discovery of New Structures: The electron microscope has enabled the discovery of new cellular structures that were previously unknown or poorly understood. This has expanded our knowledge of cell biology and opened up new avenues for research.
- Advancements in Medicine: The detailed images produced by electron microscopes have contributed to advancements in medical research and the development of new treatments for various diseases. By studying cells at a more detailed level, scientists can better understand disease processes and develop targeted therapies.
Overall, the development of the electron microscope has revolutionized the field of cell biology by providing researchers with the ability to visualize cellular structures at a much higher resolution, leading to new discoveries and advancements in our understanding of cells and their functions.
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Read the following text and answer the following questions on the basis of the same:Electron Microscope Electron microscopes use electrons to illuminate a sample. In Transmission Electron Microscopy (TEM), electrons pass through the sample and illuminate film or a digital camera.Resolution in microscopy is limited to about half of the wavelength of the illumination source used to image the sample. Using visible light the best resolution that can be achieved by microscopes is about ~200 nm. Louis de Broglie showed that every particle or matter propagates like a wave. The wavelength of propagating electrons at a given accelerating voltage can be determined byThus, the wavelength of electrons is calculated to be 3.88 pm when the microscope is operated at 100 keV, 2. 74 pm at 200 keV and 2.24 pm at 300 keV. However, because the velocities of electrons in an electron microscope reach about 70% the speed of light with an accelerating voltage of 200 keV, there are relativistic effects on these electrons. Due to this effect, the wavelength at 100 keV, 200 keV and 300 keV in electron microscopes is 3.70 pm, 2.51 pm and 1.96 pm, respectively.Anyhow, the wavelength of electrons is much smaller than that of photons (2.5 pm at 200 keV). Thus if electron wave is used to illuminate the sample, the resolution of an electron microscope theoretically becomes unlimited. Practically, the resolution is limited to ~0.1 nm due to the objective lens system in electron microscopes. Thus, electron microscopy can resolve subcellular structures that could not be visualized using standard fluorescence microscopy.Q. Wavelength of electron as wave at accelerating voltage 200 keV is

A major breakthrough in the studies of cells came with the development of electron microscope because?
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