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Read the following text and answer the following question on the basis of the same:
The term biotechnology refers to the use of living organisms or their products to modify human health and their human environment. For example, ‘testtube’ programme, synthesis of a gene or correcting a defective gene are all part of biotechnology. The basis of modern biotechnology is genetic engineering and maintenance of sterile conditions. Genetic engineering is the technique that alter the chemistry of genetic material i.e. DNA and RNA, then this genetic material is introduced into host organisms, which alter the phenotype of the host organism.
Q. The recognition sequence of the first restriction enzyme isolated was ________ base pair long.
  • a)
    four
  • b)
    six
  • c)
    five
  • d)
    two
Correct answer is option 'B'. Can you explain this answer?
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Read the following text and answer the following question on the basi...
ECoRI is isolated as the first restriction of the GAATAC nucleotide base pair.
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The recognition sequence of the first restriction enzyme isolated was six base pair long.

The first restriction enzyme isolated was HindII, which recognizes a specific DNA sequence and cleaves it at that site. The recognition sequence of HindII is 6 base pairs long. This means that HindII recognizes a specific sequence of 6 nucleotides in the DNA molecule.

Restriction enzymes and their recognition sequences:
- Restriction enzymes are proteins that can recognize specific DNA sequences and cut the DNA at those sequences.
- Each restriction enzyme recognizes a specific sequence of nucleotides, usually 4 to 6 base pairs long.
- The recognition sequence is usually palindromic, meaning it reads the same forward and backward on both DNA strands.
- Once the restriction enzyme recognizes its specific sequence, it cuts the DNA at that site.

HindII and its recognition sequence:
- HindII was the first restriction enzyme to be isolated and characterized.
- It recognizes the DNA sequence 5'-GTPyPuAC-3', where G is guanine, T is thymine, P is any purine (A or G), and y is any pyrimidine (C or T).
- This sequence is palindromic, as it reads the same on both DNA strands when the orientation is reversed.
- HindII cuts the DNA between the 3rd and 4th nucleotides on each strand, generating blunt ends.

Significance of restriction enzymes:
- Restriction enzymes have revolutionized molecular biology and biotechnology.
- They are used in DNA cloning, genetic engineering, and various other applications.
- By cutting DNA at specific sequences, restriction enzymes allow scientists to manipulate and analyze DNA fragments.
- They are used to create recombinant DNA molecules, insert genes into plasmids, and study gene function.

In conclusion, the recognition sequence of the first restriction enzyme isolated, HindII, was six base pairs long. This discovery paved the way for the field of genetic engineering and the use of restriction enzymes in biotechnology.
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Read the following text and answer the following question on the basis of the same:The term biotechnology refers to the use of living organisms or their products to modify human health and their human environment. For example, ‘testtube’ programme, synthesis of a gene or correcting a defective gene are all part of biotechnology. The basis of modern biotechnology is genetic engineering and maintenance of sterile conditions. Genetic engineering is the technique that alter the chemistry of genetic material i.e. DNA and RNA, then this genetic material is introduced into host organisms, which alter the phenotype of the host organism.Q. The recognition sequence of the first restriction enzyme isolated was ________ base pair long.a)fourb)sixc)fived)twoCorrect answer is option 'B'. Can you explain this answer?
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