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Read the following statements and select the correct ones.
(i) Electrophoresis is a technique used for the separation of molecules based on their size and charge
(ii) Plasmids are extra-chromosomal, self-replicating, usually circular, double-stranded DNA molecules found naturally in many bacteria and also in some yeast
(iii) It is not advisable to use an exonuclease enzyme while producing a recombinant DNA molecule
(iv) In EcoRI, the roman numeral I indicates that it was the first enzyme isolated from E.coli RY 13
  • a)
    (i) and (ii)
  • b)
    (iii) and (iv)
  • c)
    (i), (ii) and (iv)
  • d)
    (i), (ii), (iii) and (iv)
Correct answer is option 'D'. Can you explain this answer?
Verified Answer
Read the following statements and select the correct ones.(i) Electrop...
  • Electrophoresis is a technique used for the separation of molecules based on their size and charge.
  • Plasmids are extra-chromosomal, self-replicating, usually circular, double-stranded DNA molecules found naturally in many bacteria and also in some yeast.
  • It is not advisable to use an exonuclease enzyme while producing a recombinant DNA molecule.
  • In EcoRI, the roman numeral I indicates that it was the first enzyme isolated from E.coli RY 13.
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Most Upvoted Answer
Read the following statements and select the correct ones.(i) Electrop...
Electrophoresis, plasmids, exonuclease enzyme and EcoRI are all important concepts in molecular biology. Let's understand each statement and why option 'D' is the correct answer.

(i) Electrophoresis is a technique used for the separation of molecules based on their size and charge:
Electrophoresis is a laboratory technique used to separate macromolecules like DNA, RNA, and proteins based on their size and charge. An electric field is applied to a gel matrix containing the molecules, causing them to migrate towards the opposite electrode. Smaller molecules move faster and travel farther compared to larger ones. This technique is widely used in molecular biology and biochemistry for various applications like DNA fingerprinting, protein analysis, and genotyping.

(ii) Plasmids are extra-chromosomal, self-replicating, usually circular, double-stranded DNA molecules found naturally in many bacteria and also in some yeast:
Plasmids are small, circular DNA molecules that exist independently of the chromosomal DNA in bacterial and yeast cells. They can replicate independently and carry genes that provide certain advantages to the host cell, such as antibiotic resistance, virulence factors, or metabolic enzymes. Plasmids are widely used in genetic engineering and biotechnology as vectors for gene transfer and expression in different organisms.

(iii) It is not advisable to use an exonuclease enzyme while producing a recombinant DNA molecule:
Exonucleases are enzymes that cleave nucleotides from the ends of DNA or RNA molecules. In molecular biology, they are used for various applications like DNA sequencing, cloning, and mutagenesis. However, when producing a recombinant DNA molecule, which involves joining two different DNA fragments, using an exonuclease enzyme can result in unwanted degradation of the DNA ends and loss of the desired sequence. Therefore, other enzymes like restriction endonucleases or ligases are preferred for recombinant DNA technology.

(iv) In EcoRI, the roman numeral I indicates that it was the first enzyme isolated from E.coli RY 13:
EcoRI is a type of restriction endonuclease enzyme that recognizes and cleaves DNA at a specific nucleotide sequence, usually a palindromic one. It was first isolated from Escherichia coli strain RY 13 in the 1970s by a group of researchers, and the Roman numeral I indicates that it was the first enzyme discovered in that strain. EcoRI is widely used in molecular biology for various applications like DNA cloning, gene expression, and mapping.

Therefore, the correct answer is option 'D' as all four statements are true and relevant to molecular biology.
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Read the following statements and select the correct ones.(i) Electrophoresis is a technique used for the separation of molecules based on their size and charge(ii) Plasmids are extra-chromosomal, self-replicating, usually circular, double-stranded DNA molecules found naturally in many bacteria and also in some yeast(iii) It is not advisable to use an exonuclease enzyme while producing a recombinant DNA molecule(iv) In EcoRI, the roman numeral I indicates that it was the first enzyme isolated from E.coli RY 13a)(i) and (ii)b)(iii) and (iv)c)(i), (ii) and (iv)d)(i), (ii), (iii) and (iv)Correct answer is option 'D'. Can you explain this answer?
Question Description
Read the following statements and select the correct ones.(i) Electrophoresis is a technique used for the separation of molecules based on their size and charge(ii) Plasmids are extra-chromosomal, self-replicating, usually circular, double-stranded DNA molecules found naturally in many bacteria and also in some yeast(iii) It is not advisable to use an exonuclease enzyme while producing a recombinant DNA molecule(iv) In EcoRI, the roman numeral I indicates that it was the first enzyme isolated from E.coli RY 13a)(i) and (ii)b)(iii) and (iv)c)(i), (ii) and (iv)d)(i), (ii), (iii) and (iv)Correct answer is option 'D'. Can you explain this answer? for NEET 2024 is part of NEET preparation. The Question and answers have been prepared according to the NEET exam syllabus. Information about Read the following statements and select the correct ones.(i) Electrophoresis is a technique used for the separation of molecules based on their size and charge(ii) Plasmids are extra-chromosomal, self-replicating, usually circular, double-stranded DNA molecules found naturally in many bacteria and also in some yeast(iii) It is not advisable to use an exonuclease enzyme while producing a recombinant DNA molecule(iv) In EcoRI, the roman numeral I indicates that it was the first enzyme isolated from E.coli RY 13a)(i) and (ii)b)(iii) and (iv)c)(i), (ii) and (iv)d)(i), (ii), (iii) and (iv)Correct answer is option 'D'. Can you explain this answer? covers all topics & solutions for NEET 2024 Exam. Find important definitions, questions, meanings, examples, exercises and tests below for Read the following statements and select the correct ones.(i) Electrophoresis is a technique used for the separation of molecules based on their size and charge(ii) Plasmids are extra-chromosomal, self-replicating, usually circular, double-stranded DNA molecules found naturally in many bacteria and also in some yeast(iii) It is not advisable to use an exonuclease enzyme while producing a recombinant DNA molecule(iv) In EcoRI, the roman numeral I indicates that it was the first enzyme isolated from E.coli RY 13a)(i) and (ii)b)(iii) and (iv)c)(i), (ii) and (iv)d)(i), (ii), (iii) and (iv)Correct answer is option 'D'. Can you explain this answer?.
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