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Three micrograms of a circular plasmid of 4200 bp was digested with a restriction enzyme and subjected to agarose gel electrophoresis. Five DNA fragments of different sizes were observed and their sizes summed up to 4200 bp. The number of picomoles of DNA ends generated after complete digestion with the enzyme is _______.
(Given: average molecular weight of each base pair is 660 Da)
(Given: average molecular weight of each base pair is 660 Da)
Correct answer is between '10,11'. Can you explain this answer?
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Three micrograms of a circular plasmid of 4200 bp was digested with a ...
To determine the number of picomoles of DNA ends generated after complete digestion with the enzyme, we need to first calculate the total number of base pairs in the circular plasmid and then determine the number of fragments generated.
1. Calculate the total number of base pairs:
Given that the circular plasmid is 4200 bp, we can directly use this value.
2. Determine the number of fragments generated:
We are told that five DNA fragments of different sizes were observed, and their sizes summed up to 4200 bp. This means that the circular plasmid was cut into five fragments.
3. Calculate the size of each fragment:
To find the size of each fragment, we can divide the total number of base pairs (4200 bp) by the number of fragments (5). Each fragment will have an average size of 840 bp.
4. Calculate the number of moles of DNA ends:
To calculate the number of moles of DNA ends, we need to convert the size of each fragment from base pairs to grams. Given that the average molecular weight of each base pair is 660 Da, we can use this value to convert base pairs to grams.
- Convert the size of each fragment from base pairs to grams:
840 bp * (660 Da/bp) = 554,400 Da
- Convert grams to moles:
554,400 Da * (1 mol/6.022 x 10^23 Da) = 9.2 x 10^-19 mol
5. Calculate the number of picomoles of DNA ends:
To convert moles to picomoles, we multiply by 10^12.
9.2 x 10^-19 mol * (10^12 pmol/1 mol) = 9.2 x 10^-7 pmol
Therefore, the number of picomoles of DNA ends generated after complete digestion with the enzyme is approximately 9.2 x 10^-7 pmol, which falls between 10 and 11.
Note: The calculation assumes that the digestion with the restriction enzyme is complete and all the fragments are of equal length.
1. Calculate the total number of base pairs:
Given that the circular plasmid is 4200 bp, we can directly use this value.
2. Determine the number of fragments generated:
We are told that five DNA fragments of different sizes were observed, and their sizes summed up to 4200 bp. This means that the circular plasmid was cut into five fragments.
3. Calculate the size of each fragment:
To find the size of each fragment, we can divide the total number of base pairs (4200 bp) by the number of fragments (5). Each fragment will have an average size of 840 bp.
4. Calculate the number of moles of DNA ends:
To calculate the number of moles of DNA ends, we need to convert the size of each fragment from base pairs to grams. Given that the average molecular weight of each base pair is 660 Da, we can use this value to convert base pairs to grams.
- Convert the size of each fragment from base pairs to grams:
840 bp * (660 Da/bp) = 554,400 Da
- Convert grams to moles:
554,400 Da * (1 mol/6.022 x 10^23 Da) = 9.2 x 10^-19 mol
5. Calculate the number of picomoles of DNA ends:
To convert moles to picomoles, we multiply by 10^12.
9.2 x 10^-19 mol * (10^12 pmol/1 mol) = 9.2 x 10^-7 pmol
Therefore, the number of picomoles of DNA ends generated after complete digestion with the enzyme is approximately 9.2 x 10^-7 pmol, which falls between 10 and 11.
Note: The calculation assumes that the digestion with the restriction enzyme is complete and all the fragments are of equal length.
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Three micrograms of a circular plasmid of 4200 bp was digested with a restriction enzyme and subjected to agarose gel electrophoresis. Five DNA fragments of different sizes were observed and their sizes summed up to 4200 bp. The number of picomoles of DNA ends generated after complete digestion with the enzyme is _______.(Given: average molecular weight of each base pair is 660 Da)Correct answer is between '10,11'. Can you explain this answer?
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Three micrograms of a circular plasmid of 4200 bp was digested with a restriction enzyme and subjected to agarose gel electrophoresis. Five DNA fragments of different sizes were observed and their sizes summed up to 4200 bp. The number of picomoles of DNA ends generated after complete digestion with the enzyme is _______.(Given: average molecular weight of each base pair is 660 Da)Correct answer is between '10,11'. Can you explain this answer? for GATE 2024 is part of GATE preparation. The Question and answers have been prepared according to the GATE exam syllabus. Information about Three micrograms of a circular plasmid of 4200 bp was digested with a restriction enzyme and subjected to agarose gel electrophoresis. Five DNA fragments of different sizes were observed and their sizes summed up to 4200 bp. The number of picomoles of DNA ends generated after complete digestion with the enzyme is _______.(Given: average molecular weight of each base pair is 660 Da)Correct answer is between '10,11'. Can you explain this answer? covers all topics & solutions for GATE 2024 Exam. Find important definitions, questions, meanings, examples, exercises and tests below for Three micrograms of a circular plasmid of 4200 bp was digested with a restriction enzyme and subjected to agarose gel electrophoresis. Five DNA fragments of different sizes were observed and their sizes summed up to 4200 bp. The number of picomoles of DNA ends generated after complete digestion with the enzyme is _______.(Given: average molecular weight of each base pair is 660 Da)Correct answer is between '10,11'. Can you explain this answer?.
Three micrograms of a circular plasmid of 4200 bp was digested with a restriction enzyme and subjected to agarose gel electrophoresis. Five DNA fragments of different sizes were observed and their sizes summed up to 4200 bp. The number of picomoles of DNA ends generated after complete digestion with the enzyme is _______.(Given: average molecular weight of each base pair is 660 Da)Correct answer is between '10,11'. Can you explain this answer? for GATE 2024 is part of GATE preparation. The Question and answers have been prepared according to the GATE exam syllabus. Information about Three micrograms of a circular plasmid of 4200 bp was digested with a restriction enzyme and subjected to agarose gel electrophoresis. Five DNA fragments of different sizes were observed and their sizes summed up to 4200 bp. The number of picomoles of DNA ends generated after complete digestion with the enzyme is _______.(Given: average molecular weight of each base pair is 660 Da)Correct answer is between '10,11'. Can you explain this answer? covers all topics & solutions for GATE 2024 Exam. Find important definitions, questions, meanings, examples, exercises and tests below for Three micrograms of a circular plasmid of 4200 bp was digested with a restriction enzyme and subjected to agarose gel electrophoresis. Five DNA fragments of different sizes were observed and their sizes summed up to 4200 bp. The number of picomoles of DNA ends generated after complete digestion with the enzyme is _______.(Given: average molecular weight of each base pair is 660 Da)Correct answer is between '10,11'. Can you explain this answer?.
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