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Consider the following statements regarding CRISPR-Cas9:
CRISPR-Cas9 was adapted from a naturally occurring genome editing system that bacteria use as an immune defense.
Unlike other genome editing tools, CRISPRs do not need to be paired with separate cleaving enzymes.
It can help in permanently modifying genes in living cells and organisms.
Which of the statements given above is/are correct?
- a)1, 2 and 3
- b)1 and 3 only
- c)3 only
- d)1 and 2 only
Correct answer is option 'A'. Can you explain this answer?
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Most Upvoted Answer
Consider the following statements regarding CRISPR-Cas9: CRISPR-Cas...
The correct answer is option 'A': 1, 2 and 3.
Explanation:
CRISPR-Cas9 is a revolutionary gene-editing tool that has gained significant attention in recent years. It is a system that was adapted from a naturally occurring genome editing system that bacteria use as an immune defense. The CRISPR-Cas9 system allows scientists to make precise changes to the DNA of living cells and organisms, opening up new possibilities for genetic research and medical applications.
Let's examine each statement in detail:
1. CRISPR-Cas9 was adapted from a naturally occurring genome editing system that bacteria use as an immune defense:
- The CRISPR-Cas9 system is based on the natural defense mechanism of bacteria against viral infections.
- Bacteria incorporate small snippets of viral DNA into their own genome, forming what is known as CRISPR arrays.
- These CRISPR arrays, along with the Cas9 protein, provide bacteria with a means to recognize and destroy viral DNA if the same virus attacks again in the future.
- Scientists have harnessed this natural system and adapted it for use in genome editing.
2. Unlike other genome editing tools, CRISPRs do not need to be paired with separate cleaving enzymes:
- Traditional genome editing tools, such as zinc finger nucleases and TALENs, rely on the use of separate cleaving enzymes to make precise DNA cuts.
- In contrast, the CRISPR-Cas9 system uses a single RNA molecule called a guide RNA (gRNA) to target the Cas9 protein to specific DNA sequences.
- The Cas9 protein acts as both a DNA-binding protein and an enzyme that can cut the DNA at the targeted site, making it a versatile and efficient genome editing tool.
3. It can help in permanently modifying genes in living cells and organisms:
- CRISPR-Cas9 is capable of making permanent changes to the DNA sequence of cells and organisms.
- After the DNA is cut by the Cas9 protein, the cell's natural repair mechanisms come into play.
- These repair mechanisms can introduce small insertions or deletions (indels) at the site of the DNA cut, leading to gene knockout or disruption.
- Alternatively, scientists can also provide a DNA template along with the CRISPR-Cas9 system to make precise changes to the DNA sequence through a process called homology-directed repair (HDR).
In conclusion, all three statements regarding CRISPR-Cas9 are correct. It is a powerful genome editing tool adapted from a natural bacterial defense system, it does not require separate cleaving enzymes, and it can be used to permanently modify genes in living cells and organisms.
Explanation:
CRISPR-Cas9 is a revolutionary gene-editing tool that has gained significant attention in recent years. It is a system that was adapted from a naturally occurring genome editing system that bacteria use as an immune defense. The CRISPR-Cas9 system allows scientists to make precise changes to the DNA of living cells and organisms, opening up new possibilities for genetic research and medical applications.
Let's examine each statement in detail:
1. CRISPR-Cas9 was adapted from a naturally occurring genome editing system that bacteria use as an immune defense:
- The CRISPR-Cas9 system is based on the natural defense mechanism of bacteria against viral infections.
- Bacteria incorporate small snippets of viral DNA into their own genome, forming what is known as CRISPR arrays.
- These CRISPR arrays, along with the Cas9 protein, provide bacteria with a means to recognize and destroy viral DNA if the same virus attacks again in the future.
- Scientists have harnessed this natural system and adapted it for use in genome editing.
2. Unlike other genome editing tools, CRISPRs do not need to be paired with separate cleaving enzymes:
- Traditional genome editing tools, such as zinc finger nucleases and TALENs, rely on the use of separate cleaving enzymes to make precise DNA cuts.
- In contrast, the CRISPR-Cas9 system uses a single RNA molecule called a guide RNA (gRNA) to target the Cas9 protein to specific DNA sequences.
- The Cas9 protein acts as both a DNA-binding protein and an enzyme that can cut the DNA at the targeted site, making it a versatile and efficient genome editing tool.
3. It can help in permanently modifying genes in living cells and organisms:
- CRISPR-Cas9 is capable of making permanent changes to the DNA sequence of cells and organisms.
- After the DNA is cut by the Cas9 protein, the cell's natural repair mechanisms come into play.
- These repair mechanisms can introduce small insertions or deletions (indels) at the site of the DNA cut, leading to gene knockout or disruption.
- Alternatively, scientists can also provide a DNA template along with the CRISPR-Cas9 system to make precise changes to the DNA sequence through a process called homology-directed repair (HDR).
In conclusion, all three statements regarding CRISPR-Cas9 are correct. It is a powerful genome editing tool adapted from a natural bacterial defense system, it does not require separate cleaving enzymes, and it can be used to permanently modify genes in living cells and organisms.
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Consider the following statements regarding CRISPR-Cas9: CRISPR-Cas9 was adapted from a naturally occurring genome editing system that bacteria use as an immune defense. Unlike other genome editing tools, CRISPRs do not need to be paired with separate cleaving enzymes. It can help in permanently modifying genes in living cells and organisms. Which of the statements given above is/are correct?a)1, 2 and 3b)1 and 3 onlyc)3 onlyd)1 and 2 onlyCorrect answer is option 'A'. Can you explain this answer?
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Consider the following statements regarding CRISPR-Cas9: CRISPR-Cas9 was adapted from a naturally occurring genome editing system that bacteria use as an immune defense. Unlike other genome editing tools, CRISPRs do not need to be paired with separate cleaving enzymes. It can help in permanently modifying genes in living cells and organisms. Which of the statements given above is/are correct?a)1, 2 and 3b)1 and 3 onlyc)3 onlyd)1 and 2 onlyCorrect answer is option 'A'. Can you explain this answer? for UPSC 2024 is part of UPSC preparation. The Question and answers have been prepared according to the UPSC exam syllabus. Information about Consider the following statements regarding CRISPR-Cas9: CRISPR-Cas9 was adapted from a naturally occurring genome editing system that bacteria use as an immune defense. Unlike other genome editing tools, CRISPRs do not need to be paired with separate cleaving enzymes. It can help in permanently modifying genes in living cells and organisms. Which of the statements given above is/are correct?a)1, 2 and 3b)1 and 3 onlyc)3 onlyd)1 and 2 onlyCorrect answer is option 'A'. Can you explain this answer? covers all topics & solutions for UPSC 2024 Exam. Find important definitions, questions, meanings, examples, exercises and tests below for Consider the following statements regarding CRISPR-Cas9: CRISPR-Cas9 was adapted from a naturally occurring genome editing system that bacteria use as an immune defense. Unlike other genome editing tools, CRISPRs do not need to be paired with separate cleaving enzymes. It can help in permanently modifying genes in living cells and organisms. Which of the statements given above is/are correct?a)1, 2 and 3b)1 and 3 onlyc)3 onlyd)1 and 2 onlyCorrect answer is option 'A'. Can you explain this answer?.
Consider the following statements regarding CRISPR-Cas9: CRISPR-Cas9 was adapted from a naturally occurring genome editing system that bacteria use as an immune defense. Unlike other genome editing tools, CRISPRs do not need to be paired with separate cleaving enzymes. It can help in permanently modifying genes in living cells and organisms. Which of the statements given above is/are correct?a)1, 2 and 3b)1 and 3 onlyc)3 onlyd)1 and 2 onlyCorrect answer is option 'A'. Can you explain this answer? for UPSC 2024 is part of UPSC preparation. The Question and answers have been prepared according to the UPSC exam syllabus. Information about Consider the following statements regarding CRISPR-Cas9: CRISPR-Cas9 was adapted from a naturally occurring genome editing system that bacteria use as an immune defense. Unlike other genome editing tools, CRISPRs do not need to be paired with separate cleaving enzymes. It can help in permanently modifying genes in living cells and organisms. Which of the statements given above is/are correct?a)1, 2 and 3b)1 and 3 onlyc)3 onlyd)1 and 2 onlyCorrect answer is option 'A'. Can you explain this answer? covers all topics & solutions for UPSC 2024 Exam. Find important definitions, questions, meanings, examples, exercises and tests below for Consider the following statements regarding CRISPR-Cas9: CRISPR-Cas9 was adapted from a naturally occurring genome editing system that bacteria use as an immune defense. Unlike other genome editing tools, CRISPRs do not need to be paired with separate cleaving enzymes. It can help in permanently modifying genes in living cells and organisms. Which of the statements given above is/are correct?a)1, 2 and 3b)1 and 3 onlyc)3 onlyd)1 and 2 onlyCorrect answer is option 'A'. Can you explain this answer?.
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