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During the process of gene amplification using PCR, if a very high temperature is not maintained in the beginning, then which of the following steps of PCR will be affected first?
  • a)
    Annealing
  • b)
    Extension
  • c)
    Denaturation
  • d)
    Ligation
Correct answer is option 'C'. Can you explain this answer?
Most Upvoted Answer
During the process of gene amplification using PCR, if a very high tem...
Denaturation -94-95oC, annealing- 50-60oC, extension- 72oC. Since maximum temperature is required during denaturation, it will be affected the most.
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During the process of gene amplification using PCR, if a very high temperature is not maintained in the beginning, then which of the following steps of PCR will be affected first?a)Annealingb)Extensionc)Denaturationd)LigationCorrect answer is option 'C'. Can you explain this answer?
Question Description
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