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Which of the following statement is incorrect with respect to polymerase chain reaction?
- a)The segment of DNA can be amplified several times.
- b)Repeated amplification is achieved by the use of a thermostable DNA polymerase isolated from a bacterium, Thermus aquaticus
- c)Multiple copies of the gene (or DNA) of interest are synthesized in vitro using two primers and the enzyme RNA polymerase
- d)It includes the process of denaturation, annealing and extension.
Correct answer is option 'C'. Can you explain this answer?
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Which of the following statement is incorrect with respect to polymera...
Incorrect Statement with respect to Polymerase Chain Reaction
The polymerase chain reaction (PCR) is a technique used to amplify a segment of DNA. It involves a series of steps that are repeated multiple times to generate millions or billions of copies of a specific DNA sequence. The incorrect statement with respect to PCR is:
c) Multiple copies of the gene (or DNA) of interest are synthesized in vitro using two primers and the enzyme RNA polymerase
Explanation
The correct enzyme used in PCR is DNA polymerase, not RNA polymerase. RNA polymerase is an enzyme that is involved in transcription, not DNA replication. The two primers used in PCR are short sequences of DNA that are complementary to the regions flanking the DNA sequence of interest. These primers serve as the starting point for DNA synthesis.
Steps Involved in Polymerase Chain Reaction
The polymerase chain reaction involves the following steps:
1. Denaturation: The double-stranded DNA is heated at a high temperature (~95°C) to separate the two strands.
2. Annealing: The temperature is lowered (~55-65°C) to allow the primers to anneal to the complementary regions on the single-stranded DNA.
3. Extension: The temperature is raised (~72°C) to activate the DNA polymerase enzyme, which adds nucleotides to the 3' end of the primers, synthesizing new DNA strands.
4. Repeat: The above three steps are repeated 20-40 times, resulting in the amplification of the original DNA sequence.
Applications of Polymerase Chain Reaction
PCR finds applications in various fields, including:
1. Medical diagnosis: PCR can be used to detect the presence of specific disease-causing organisms, such as bacteria or viruses.
2. Forensic analysis: PCR can be used to amplify DNA samples from crime scenes and identify suspects.
3. Genetic testing: PCR can be used to identify mutations or genetic variations that can cause or increase the risk of inherited diseases.
Conclusion
Polymerase chain reaction is a powerful tool in molecular biology that enables the amplification of specific DNA sequences. It involves the use of DNA polymerase, not RNA polymerase, and two primers that anneal to the regions flanking the DNA sequence of interest. The technique finds applications in various fields, including medical diagnosis, forensic analysis, and genetic testing.
The polymerase chain reaction (PCR) is a technique used to amplify a segment of DNA. It involves a series of steps that are repeated multiple times to generate millions or billions of copies of a specific DNA sequence. The incorrect statement with respect to PCR is:
c) Multiple copies of the gene (or DNA) of interest are synthesized in vitro using two primers and the enzyme RNA polymerase
Explanation
The correct enzyme used in PCR is DNA polymerase, not RNA polymerase. RNA polymerase is an enzyme that is involved in transcription, not DNA replication. The two primers used in PCR are short sequences of DNA that are complementary to the regions flanking the DNA sequence of interest. These primers serve as the starting point for DNA synthesis.
Steps Involved in Polymerase Chain Reaction
The polymerase chain reaction involves the following steps:
1. Denaturation: The double-stranded DNA is heated at a high temperature (~95°C) to separate the two strands.
2. Annealing: The temperature is lowered (~55-65°C) to allow the primers to anneal to the complementary regions on the single-stranded DNA.
3. Extension: The temperature is raised (~72°C) to activate the DNA polymerase enzyme, which adds nucleotides to the 3' end of the primers, synthesizing new DNA strands.
4. Repeat: The above three steps are repeated 20-40 times, resulting in the amplification of the original DNA sequence.
Applications of Polymerase Chain Reaction
PCR finds applications in various fields, including:
1. Medical diagnosis: PCR can be used to detect the presence of specific disease-causing organisms, such as bacteria or viruses.
2. Forensic analysis: PCR can be used to amplify DNA samples from crime scenes and identify suspects.
3. Genetic testing: PCR can be used to identify mutations or genetic variations that can cause or increase the risk of inherited diseases.
Conclusion
Polymerase chain reaction is a powerful tool in molecular biology that enables the amplification of specific DNA sequences. It involves the use of DNA polymerase, not RNA polymerase, and two primers that anneal to the regions flanking the DNA sequence of interest. The technique finds applications in various fields, including medical diagnosis, forensic analysis, and genetic testing.
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Which of the following statement is incorrect with respect to polymera...
Each option is correct except answer c
in question it ask incorrect answer because in PCR thermostable DNA polymerase (taq polymerase used which is obtained from thermostable bacteria ) but option c say about RNA pol so C is appropriate answer
in question it ask incorrect answer because in PCR thermostable DNA polymerase (taq polymerase used which is obtained from thermostable bacteria ) but option c say about RNA pol so C is appropriate answer
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Which of the following statement is incorrect with respect to polymerase chain reaction?a)The segment of DNA can be amplified several times.b)Repeated amplification is achieved by the use of a thermostable DNA polymerase isolated from a bacterium, Thermus aquaticusc)Multiple copies of the gene (or DNA) of interest are synthesized in vitro using two primers and the enzyme RNA polymerase d)It includes the process of denaturation, annealing and extension. Correct answer is option 'C'. Can you explain this answer?
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Which of the following statement is incorrect with respect to polymerase chain reaction?a)The segment of DNA can be amplified several times.b)Repeated amplification is achieved by the use of a thermostable DNA polymerase isolated from a bacterium, Thermus aquaticusc)Multiple copies of the gene (or DNA) of interest are synthesized in vitro using two primers and the enzyme RNA polymerase d)It includes the process of denaturation, annealing and extension. Correct answer is option 'C'. Can you explain this answer? for NEET 2024 is part of NEET preparation. The Question and answers have been prepared according to the NEET exam syllabus. Information about Which of the following statement is incorrect with respect to polymerase chain reaction?a)The segment of DNA can be amplified several times.b)Repeated amplification is achieved by the use of a thermostable DNA polymerase isolated from a bacterium, Thermus aquaticusc)Multiple copies of the gene (or DNA) of interest are synthesized in vitro using two primers and the enzyme RNA polymerase d)It includes the process of denaturation, annealing and extension. Correct answer is option 'C'. Can you explain this answer? covers all topics & solutions for NEET 2024 Exam. Find important definitions, questions, meanings, examples, exercises and tests below for Which of the following statement is incorrect with respect to polymerase chain reaction?a)The segment of DNA can be amplified several times.b)Repeated amplification is achieved by the use of a thermostable DNA polymerase isolated from a bacterium, Thermus aquaticusc)Multiple copies of the gene (or DNA) of interest are synthesized in vitro using two primers and the enzyme RNA polymerase d)It includes the process of denaturation, annealing and extension. Correct answer is option 'C'. Can you explain this answer?.
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