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Assertion: The separated DNA fragments can be visualized only after staining the DNA with a compound known as ethidium bromide followed by exposure to UV radiation.
Reason: We can see bright red coloured bands of DNA in an ethidium bromide-stained gel exposed to UV light.
Reason: We can see bright red coloured bands of DNA in an ethidium bromide-stained gel exposed to UV light.
- a)Both assertion and reason are correct.
- b)Assertion is correct but reason does not explain the assertion
- c)Both assertion and reason are incorrect.
- d)Assertion is correct but reason is incorrect.
Correct answer is option 'D'. Can you explain this answer?
Most Upvoted Answer
Assertion: The separated DNA fragments can be visualized only after st...
The separated DNA fragments can be visualized only after staining the DNA with a compound known as ethidium bromide followed by exposure to UV radiation. We can see bright orange coloured bands of DNA in aethidium bromide stained gel exposed to UV light.
Topic in NCERT: Separation and isolation of DNA fragments
Line in NCERT: "The separated DNA fragments can be visualised only after staining the DNA with a compound known as ethidium bromide followed by exposure to UV radiation (you cannot see pure DNA fragments in the visible light and without staining). You can see bright orange coloured bands of DNA in an ethidium bromide stained gel exposed to UV light."
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Assertion: The separated DNA fragments can be visualized only after st...
Understanding the Assertion and Reason
The assertion and reason provided relate to the visualization of DNA fragments in the context of gel electrophoresis.
Assertion Explained
- The assertion states that separated DNA fragments can only be visualized after staining with ethidium bromide and exposure to UV radiation.
- This is correct because ethidium bromide intercalates between the bases of DNA.
- When exposed to UV light, the ethidium bromide fluoresces, allowing the DNA bands to be seen.
Reason Clarified
- The reason claims that we see bright red colored bands of DNA in an ethidium bromide-stained gel under UV light.
- This statement is partially incorrect because the fluorescence emitted by ethidium bromide is actually orange, not red.
Conclusion of the Options
- Since the assertion about the need for ethidium bromide and UV light for visualization of DNA fragments is correct, but the reason for the color is incorrect, the correct answer is option 'D'.
- Thus, the assertion is correct, while the reason is incorrect.
Key Takeaways
- Ethidium bromide is essential for visualizing DNA in gel electrophoresis.
- The fluorescence observed under UV light is orange, not red.
- Understanding the correct color and its implications is crucial in molecular biology.
This differentiation is critical for accurate scientific communication and comprehension in fields like genetics and biotechnology.
The assertion and reason provided relate to the visualization of DNA fragments in the context of gel electrophoresis.
Assertion Explained
- The assertion states that separated DNA fragments can only be visualized after staining with ethidium bromide and exposure to UV radiation.
- This is correct because ethidium bromide intercalates between the bases of DNA.
- When exposed to UV light, the ethidium bromide fluoresces, allowing the DNA bands to be seen.
Reason Clarified
- The reason claims that we see bright red colored bands of DNA in an ethidium bromide-stained gel under UV light.
- This statement is partially incorrect because the fluorescence emitted by ethidium bromide is actually orange, not red.
Conclusion of the Options
- Since the assertion about the need for ethidium bromide and UV light for visualization of DNA fragments is correct, but the reason for the color is incorrect, the correct answer is option 'D'.
- Thus, the assertion is correct, while the reason is incorrect.
Key Takeaways
- Ethidium bromide is essential for visualizing DNA in gel electrophoresis.
- The fluorescence observed under UV light is orange, not red.
- Understanding the correct color and its implications is crucial in molecular biology.
This differentiation is critical for accurate scientific communication and comprehension in fields like genetics and biotechnology.
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Assertion: The separated DNA fragments can be visualized only after staining the DNA with a compound known as ethidium bromide followed by exposure to UV radiation.Reason:We can see bright red coloured bands of DNA in an ethidium bromide-stained gel exposed to UV light.a)Both assertion and reason are correct.b)Assertion is correct but reason does not explain the assertionc)Both assertion and reason are incorrect.d)Assertion is correct but reason is incorrect.Correct answer is option 'D'. Can you explain this answer? for NEET 2025 is part of NEET preparation. The Question and answers have been prepared according to the NEET exam syllabus. Information about Assertion: The separated DNA fragments can be visualized only after staining the DNA with a compound known as ethidium bromide followed by exposure to UV radiation.Reason:We can see bright red coloured bands of DNA in an ethidium bromide-stained gel exposed to UV light.a)Both assertion and reason are correct.b)Assertion is correct but reason does not explain the assertionc)Both assertion and reason are incorrect.d)Assertion is correct but reason is incorrect.Correct answer is option 'D'. Can you explain this answer? covers all topics & solutions for NEET 2025 Exam. Find important definitions, questions, meanings, examples, exercises and tests below for Assertion: The separated DNA fragments can be visualized only after staining the DNA with a compound known as ethidium bromide followed by exposure to UV radiation.Reason:We can see bright red coloured bands of DNA in an ethidium bromide-stained gel exposed to UV light.a)Both assertion and reason are correct.b)Assertion is correct but reason does not explain the assertionc)Both assertion and reason are incorrect.d)Assertion is correct but reason is incorrect.Correct answer is option 'D'. Can you explain this answer?.
Assertion: The separated DNA fragments can be visualized only after staining the DNA with a compound known as ethidium bromide followed by exposure to UV radiation.Reason:We can see bright red coloured bands of DNA in an ethidium bromide-stained gel exposed to UV light.a)Both assertion and reason are correct.b)Assertion is correct but reason does not explain the assertionc)Both assertion and reason are incorrect.d)Assertion is correct but reason is incorrect.Correct answer is option 'D'. Can you explain this answer? for NEET 2025 is part of NEET preparation. The Question and answers have been prepared according to the NEET exam syllabus. Information about Assertion: The separated DNA fragments can be visualized only after staining the DNA with a compound known as ethidium bromide followed by exposure to UV radiation.Reason:We can see bright red coloured bands of DNA in an ethidium bromide-stained gel exposed to UV light.a)Both assertion and reason are correct.b)Assertion is correct but reason does not explain the assertionc)Both assertion and reason are incorrect.d)Assertion is correct but reason is incorrect.Correct answer is option 'D'. Can you explain this answer? covers all topics & solutions for NEET 2025 Exam. Find important definitions, questions, meanings, examples, exercises and tests below for Assertion: The separated DNA fragments can be visualized only after staining the DNA with a compound known as ethidium bromide followed by exposure to UV radiation.Reason:We can see bright red coloured bands of DNA in an ethidium bromide-stained gel exposed to UV light.a)Both assertion and reason are correct.b)Assertion is correct but reason does not explain the assertionc)Both assertion and reason are incorrect.d)Assertion is correct but reason is incorrect.Correct answer is option 'D'. Can you explain this answer?.
Solutions for Assertion: The separated DNA fragments can be visualized only after staining the DNA with a compound known as ethidium bromide followed by exposure to UV radiation.Reason:We can see bright red coloured bands of DNA in an ethidium bromide-stained gel exposed to UV light.a)Both assertion and reason are correct.b)Assertion is correct but reason does not explain the assertionc)Both assertion and reason are incorrect.d)Assertion is correct but reason is incorrect.Correct answer is option 'D'. Can you explain this answer? in English & in Hindi are available as part of our courses for NEET.
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ample number of questions to practice Assertion: The separated DNA fragments can be visualized only after staining the DNA with a compound known as ethidium bromide followed by exposure to UV radiation.Reason:We can see bright red coloured bands of DNA in an ethidium bromide-stained gel exposed to UV light.a)Both assertion and reason are correct.b)Assertion is correct but reason does not explain the assertionc)Both assertion and reason are incorrect.d)Assertion is correct but reason is incorrect.Correct answer is option 'D'. Can you explain this answer? tests, examples and also practice NEET tests.
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