During agarose gel, electrophoresis DNA fragments move towards the anode. This is because _________.

a)
Anode is negatively charged.

b)
DNA moves in random direction.

c)
DNA is negatively charged molecules.

d)
DNA is positively charged molecules.

Correct answer is option 'C'. Can you explain this answer?

Question

During agarose gel, electrophoresis DNA fragments move towards the anode. This is because _________.

a)
Anode is negatively charged.
b)
DNA moves in random direction.
c)
DNA is negatively charged molecules.
d)
DNA is positively charged molecules.

Correct answer is option 'C'. Can you explain this answer?

Answer

A DNA fragment contains phosphate groups which have a negative charge. Hence DNA fragment are negatively charged there by moving towards anode under the influence of an electric field during gel electrophoresis.
The use of agarose gel electrophoresis revolutionized the separation of DNA. The phosphate is a back bone of DNA and RNA molecules is negatively charged, therefore when placed in an electric field DNA fragment will migrate to the positively charged anode.
Option ' C ' is correct answer.

Answer

This is coz DNA fragments are negatively charged hence it moves towards anode which is positively charged

Answer

Ans is c because as anode is positively charged and DNA is negatively charged so DNA moves towards anode.

Answer

In gel electrophoresis, DNA molecules moves towards positively charged anode because DNA is negatively charged molecules. The molecules separate out due to difference in molecular weight.##@

Answer

DNA is negatively charged therefore it moves towards anode (which is positively charged)

Answer

The reason is hidden in the soln
i.e., DNA is a -vely charged molecule and anode is +vely charged

Answer

They move towards anode electrode it is only possible when DNA is negatively cgarged

Answer

Explanation:

During agarose gel electrophoresis, DNA fragments move towards the anode (positive electrode). This is because DNA is made up of negatively charged molecules. The movement of DNA fragments in the gel is facilitated by an electric field that is applied across the gel.

Electrophoresis:
Electrophoresis is a technique used to separate and analyze DNA fragments based on their size. It involves the migration of charged molecules in an electric field. In agarose gel electrophoresis, an agarose gel is used as a medium through which the DNA fragments can move.

Electric Field:
When an electric field is applied across the gel, a potential difference is created between the two electrodes. The negatively charged electrode is called the cathode, and the positively charged electrode is called the anode. The electric field exerts a force on the charged DNA molecules, causing them to move.

Negatively Charged DNA:
DNA is composed of negatively charged phosphate groups in its backbone. These phosphate groups carry a negative charge due to the presence of negatively charged oxygen atoms. Since DNA is negatively charged, it is attracted to the positively charged anode during electrophoresis.

Movement Towards Anode:
The electric field causes the negatively charged DNA molecules to migrate through the gel towards the anode. The smaller DNA fragments move more quickly and travel further through the gel, while larger fragments move more slowly and remain closer to the starting point.

Size Separation:
As the DNA fragments move towards the anode, they separate based on their size. Smaller fragments encounter less resistance from the gel matrix and can move more easily through the pores, while larger fragments experience more resistance and move more slowly.

Visualization:
After electrophoresis, the DNA fragments can be visualized by staining the gel with a fluorescent dye or using ethidium bromide, which intercalates with the DNA and emits fluorescence under UV light. The separated bands on the gel correspond to different sizes of DNA fragments.

In conclusion, during agarose gel electrophoresis, DNA fragments move towards the anode because DNA is composed of negatively charged molecules. The electric field applied across the gel exerts a force on the negatively charged DNA, causing it to migrate towards the positively charged anode. This movement allows for the separation of DNA fragments based on their size.

Similar NEET Doubts

A: During gel electrophoresis, the DNA fragments move towards the anode.R: DNA fragments are negatively charged molecules.

Gel electrophoresis is used for

Gel electrophoresis is used for

Gel electrophoresis is used for

Gel electrophoresis is used for

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