The particles used to coat with DNA in Biolistic gun is ofa)Tungstenb)Zincc)Heliumd)QuartzCorrect answer is option 'A'. Can you explain this answer?

Question

Answer

Gene gun bombardment is a method for the physical introduction of DNA into plant cells containing cell walls. ... The metal particlesbcommonly used for gene gun bombardment include gold, tungsten, palladium, rhodium, platinum, and iridium. They are coated with DNA, accelerated by helium gas, and bombard the plant cells.

Answer

DNA is coated with tungsten before used in biolistic gun for inserting the DNA directly into target cells for obtaining particular protein.##@

Answer

Particles used to coat with DNA in Biolistic gun is Tungsten.

Tungsten is a metal that is commonly used as the particle in Biolistic guns for DNA coating. Biolistics is a method used to introduce foreign genes or DNA into living cells. This technique involves the use of a device called a Biolistic gun, which uses high-pressure gas to accelerate particles coated with DNA into the target cells. These particles act as carriers for the DNA and deliver it into the cells.

The choice of particle used in the Biolistic gun is crucial for successful gene transfer. Tungsten is often preferred due to its high density, which allows for efficient penetration into the target cells. Its high density ensures that the particles can deliver the DNA payload effectively.

Here is a detailed explanation of why tungsten is the ideal particle choice in Biolistic guns:

1. Density: Tungsten has a high density of 19.25 g/cm³, which is higher than other commonly used particles such as gold or silver. This high density enables the tungsten particles to penetrate the cell walls and reach the cell nucleus where the DNA needs to be delivered.

2. Inertness: Tungsten is chemically inert, meaning it does not react with the DNA or the cells. This is important to ensure that the DNA remains intact and functional during the delivery process.

3. Size and shape: Tungsten particles used in Biolistic guns are typically micro-sized, ranging from 1 to 3 micrometers in diameter. They are spherical in shape, which allows for uniform and controlled delivery of the DNA into the target cells.

4. Biocompatibility: Tungsten particles have been extensively used in biological research and have been shown to be well-tolerated by cells. They do not induce significant cytotoxicity or immune response, making them suitable for use in gene delivery experiments.

In summary, tungsten is chosen as the particle for DNA coating in Biolistic guns due to its high density, inertness, controlled size and shape, and biocompatibility. These properties ensure efficient and effective delivery of DNA into target cells, making tungsten an ideal choice for gene transfer experiments.

Answer

Tungsten.

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