All questions of Microbiology for Biotechnology Engineering (BT) Exam

Gentamycin is an antibiotic, which is produced by
  • a)
    Bacillus sp.
  • b)
    Cephalosporin sp.
  • c)
    Micromonospora sp.  
  • d)
    Streptomyces sp.
Correct answer is option 'C'. Can you explain this answer?

Gentamycin is an antibiotic produced by the bacteria belonging to Micromonospora sp. It inhibits translation by inhibiting 16s rRNA.

In a microbial culture, the maximum synthesis of antibiotics occurs in
  • a)
    Stationary phase
  • b)
    Exponential phase
  • c)
    Death phase
  • d)
    Lag phase
Correct answer is option 'A'. Can you explain this answer?

Maximum synthesis of antibiotics in a microbial culture occurs in stationary phase because in stationary phase, nutrition and space become limitation. So to prevent excess growth in limited resources  and in this stress condition, microbes starts producing antibiotics (as a survival mechanism).

Which of the following is/are the characteristic(s) of exotoxin?
  • a)
    They are secreted out of the cell
  • b)
    They can be converted to toxoids and used as vaccines
  • c)
    They are heat labile, and get inactivated rapidly at 60 to 80ºC
  • d)
    Their chemical nature is lipopolysaccharide
Correct answer is option 'A,B,C'. Can you explain this answer?

Anagha Bajaj answered
Exotoxins are produced by some species of gram positive and gram negative bacteria.
    - They are secreted out of the cell
    - They are heat labile, and get inactivated rapidly at 60 to 80ºC
    - They are polypeptides
    - They can be converted to toxoids and used as vaccines. 

Select the disease(s) caused by bacteria.
  • a)
    Tetanus
  • b)
    Anthrax
  • c)
    Whooping cough
  • d)
    Meningococcal meningitis
Correct answer is option 'A,B,C,D'. Can you explain this answer?

Tetanus is caused by Clostridium tetani
    Anthrax is caused by Bacillus anthracis
    Whooping cough is caused by Bordetella pertusis    
    Meningococcal meningitis caused by Bordetella pertusis

Correct sequence of events that occur during lytic cycle of bacteriophage infection.
P.    Synthesis of viral nucleic acid and protein
Q.    Release of new viruses by lysis of host cell
R.    Attachment of virus on the surface of bacteria
S.    Assembly and packaging of new virus
T.    Penetration of viral DNA into the host
  • a)
    RSTPQ
  • b)
    RTPSQ
  • c)
    STRPQ
  • d)
    TRPSQ
Correct answer is option 'B'. Can you explain this answer?

Anisha Pillai answered
Sequence of events that occur during lytic cycle of bacteriophage infection is,
(i)    Virus attaches on to the surface of the host bacterium.
(ii)    Penetration of viral DNA into the host
(iii)    Synthesis of viral nucleic acid and protein
(iv)    Assembly and packaging of new virus
(v)    Release of new virus by lysis of the host cell

Dipicolinic acid is a component of the bacterial
  • a)
    Cell wall
  • b)
    Cell membrane
  • c)
    Capsule
  • d)
    Endospore
Correct answer is option 'D'. Can you explain this answer?

Jaya Sen answered
The core of the endospore plays an important role in resistance from various unfavorable conditions. Dipicolinic acid combined with calcium ions is present in the core & plays a part in resistance.

A subunit of virus protein coat is called as
  • a)
    Capsid
  • b)
    Nucleocapsid
  • c)
    Icosahedran
  • d)
    Capsomer
Correct answer is option 'D'. Can you explain this answer?

The capsomer is a subunit of the capsid , an outer covering of protein that protects the genetic material of a virus. Capsomers self assemble to form the capsid.

Creutzfeldt Jacob disease in humans is caused by
  • a)
    Viruses
  • b)
    Viriod
  • c)
    Prion
  • d)
    Bacteriu
Correct answer is option 'C'. Can you explain this answer?

Chirag Verma answered
Creutzfeldt-Jakob disease (CJD) is caused by an abnormal infectious protein in the brain called a prion. Proteins are molecules made up of amino acids that help the cells in our body function.

Which amongst the following is an example of monotrichous bacteria?
  • a)
    Vibrio cholera
  • b)
    S. aureus
  • c)
    E.coli
  • d)
    Salmonella typhi
Correct answer is option 'A'. Can you explain this answer?

Shivani Mehta answered
Among given options, S. aureus is non motile and hence does not have flagella, E.coli and S. typhi both have peritrichous arrangement of flagella. Only V. cholera has monotrichous arrangement of flagella.

Which of the following statements is/are correct for cyanobacteria ?
  • a)
    They can not grow in a mineral medium exposed to light and air
  • b)
    Usually filamentous forms are involved in N2 fixation
  • c)
    They can perform oxygenic photosynthesis
  • d)
    N2 fixation occurs in heterocyst
Correct answer is option 'B,C,D'. Can you explain this answer?

Cyanobacteria, a group of photosynthetic bacteria can grow easily in a medium exposed to light and air. They perform oxygenic photosynthesis. Some of them are nitrogen fixing in anaerobic conditions but can grow in aerobic conditions. 

In which phase of bacterial growth, cryptic growth occurs?
  • a)
    Log phase
  • b)
    Lag Phase
  • c)
    Death phase
  • d)
    Stationary phase
Correct answer is option 'C'. Can you explain this answer?

Athul Menon answered
Cryptic growth means hidden growth of some of individual cells in a starving bacteria in death phase. The majority of cells do not grow at all in death phase but a few cells are able to multiply because these cells use product of lysis of dying cells.

The properties of an episome includes.
  • a)
    They can exist as an independent entity.
  • b)
    They can exist after integration into the host chromosome
  • c)
    They contain tra operon.
  • d)
    They have no role in horizontal gene transfer
Correct answer is option 'A,B,C'. Can you explain this answer?

Sneha Menon answered
An episome is that type of plasmid that can exist both, as an independent entity as well as after integrating into the host’s chromosome. They contain tra operon, where genes are responsible for the formation of the sex pili & the transfer of gene. Plasmids are necessary for conjugation (which is a type of horizontal gene transfer)

A bacteria divides after every 45 minutes. If a culture containing 107 cells per ml is grown for 180 minutes, what will be the cell concentration per ml after 180 minutes?
  • a)
    1.6 × 108
  • b)
    3.2 × 108
  • c)
    4.8 × 106
  • d)
    128 × 107
Correct answer is option 'A'. Can you explain this answer?

Aditi Basak answered
Dividing time is 45 minutes. Total time is 180 minutes
Number of generations after 180 minutes = 180 45 = 4
Now applying the formula
N = N0 x 2n where N = Final number of cells., N0 = Initial number of cells
N = number of generations

Match the given substance with the correct method of its sterilization
  • a)
    P-ii, Q-iv, R-i, S-iii   
  • b)
    P-ii, Q-i, R-iv, S-iii
  • c)
    P-iv, Q-ii, R-iii, S-i
  • d)
    P-iv, Q-iii, R-i, S-ii
Correct answer is option 'A'. Can you explain this answer?

Sinjini Singh answered
Culture media is always autoclaved before using. Serum, being highly heat sensitive is always filtered to remove any microbes. Heat sensitive materials like beer is sterilized by pasteurization. Hormones are cold-sterilized using gamma-radiation. 

Whopping cough is caused by
  • a)
    Helicobacter pylori
  • b)
    Haemophilus aegyptius
  • c)
    Bordetella pertusis   
  • d)
    Campylobacter jejuni.
Correct answer is option 'C'. Can you explain this answer?

Akash Kulkarni answered
Causes. Pertussis, a respiratory illness commonly known as whooping cough, is a very contagious disease caused by a type of bacteria called Bordetella pertussis. These bacteria attach to the cilia (tiny, hair-like extensions) that line part of the upper respiratory system.

Plasmids are functionally involved in which of the following?
  • a)
    Transduction   
  • b)
    Synthesis of pili
  • c)
    Transfer of chromosomal genes
  • d)
    Transfer of drug resistance.
Correct answer is option 'B,C,D'. Can you explain this answer?

Stuti Patel answered
The tra genes present in the plasmid are responsible for the formation of sex pili & the transfer of chromosomal genes from F+/Hfr/F’ cells to F- cells. The transfer of a copy of resistance plasmid results in the transfer of drug resistance genes as well. Transduction is mediated by virus and their is no role of plasmid in transduction.

Find out the mismatch from the following.
  • a)
  • b)
  • c)
  • d)
Correct answer is option 'D'. Can you explain this answer?

Umesh Singh answered
Aerotolerant anaerobes grow equally well in the presence or absence of oxygen, eg. Streptococcus pyrogenes.

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