Not Required Technique for DNA Fingerprinting:

Zinc Finger Analysis is NOT required for any of the techniques of DNA fingerprinting available at present.

Explanation:

DNA fingerprinting is a technique used to identify individuals by analyzing their DNA. It has several techniques, such as Polymerase chain reaction (PCR), Restriction enzymes, DNA-DNA hybridization, and Short Tandem Repeats (STRs).

Polymerase chain reaction (PCR): PCR is a technique used to amplify small amounts of DNA into a larger amount that can be analyzed. It is used in DNA fingerprinting to amplify specific regions of DNA that contain variable numbers of tandem repeats (VNTRs) or short tandem repeats (STRs).

Restriction enzymes: Restriction enzymes are used to cut DNA into smaller fragments that can be analyzed. They recognize specific sequences of DNA and cut the DNA at those sites. The resulting fragments can be separated by size and analyzed.

DNA-DNA hybridization: DNA-DNA hybridization is a technique used to compare the similarity of DNA sequences from different individuals. It involves denaturing the DNA and then allowing it to re-anneal with a complementary strand from another individual. The degree of similarity between the two DNA samples can be determined by measuring the strength of the hybridization.

Short Tandem Repeats (STRs): STRs are regions of DNA that contain short, repeating sequences of nucleotides. They are highly variable between individuals and are used in DNA fingerprinting to identify individuals.

Zinc Finger Analysis: Zinc finger analysis is a technique used to modify DNA sequences by adding or deleting specific nucleotides. It is not used in DNA fingerprinting.

In conclusion, Zinc Finger Analysis is not required for any of the techniques of DNA fingerprinting available at present.

DNA-DNA hybridization is a technique of molecular biology which is used to measure the similarity between DNA sequences .Hence, zinc finger analysis which is related to proteins , is not required for DNA fingerprinting .
So, the correct answer is zinc finger analysis.