In-situ conservation refers to the conservation of species in their natural habitat. It includes the protection, management, and restoration of ecosystems and natural habitats to ensure the survival of species. The options given in the question are all examples of in-situ conservation except for the botanical garden.

Botanical Garden:
- A botanical garden is a place where plants are cultivated for scientific, educational, and ornamental purposes.
- While botanical gardens may have a role in ex-situ conservation (conservation of species outside their natural habitat), they are not a part of in-situ conservation.
- Plants in botanical gardens are not necessarily grown in their natural habitats and may be from different regions or countries.

Biosphere Reserve:
- A biosphere reserve is a protected area that includes one or more ecosystems and is managed to promote both conservation and sustainable development.
- It aims to conserve biodiversity while also providing opportunities for research, education, and sustainable use of natural resources.

National Park:
- A national park is a protected area that is set aside for the conservation of natural habitats and wildlife.
- It is managed for the primary purpose of protecting species and ecosystems, and for providing opportunities for recreation, education, and research.

Sanctuary:
- A sanctuary is a protected area that is set aside for the conservation of specific species or groups of species.
- It may be established for the protection of migratory birds, marine mammals, or other wildlife.

In conclusion, the correct answer is option A, botanical garden, as it is not a part of in-situ conservation.

Botanical gardens (i.e., that maintain living plant collections representing a large number of species, genera and families) are the means of ex situ conservation (i.e., conservation outside the habitats).

•Insitu conservation involves the conservation of genetic resources through their maintenance within natural or even human-made ecosystems in which they occur. It includes National parks, Sanctuaries, Biosphere reserves, Nature Reserves, Natural Monuments, Cultural Landscapes etc.