Hershey and chase experiment the bacteriophage with radioactive sulphur protein coat and normal DNA was let to infect e coli the result was no radioactive was detected in cell and in the same way when bacteriophage with normal protein coat and radioactive phosphate DNA is allowed to replicate in e coli the result was radioactive phosphate was found in new generation this proves DNA is genetic material

Experimental Differentiation of DNA and Protein by Hershey and Chase:

To differentiate between DNA and protein in their experiment, Hershey and Chase used radioactive labeling of both substances to track their movement during viral infection of bacteria. They aimed to prove that DNA, not protein, is the genetic material responsible for inheritance.

Radioactive Labeling:
- Hershey and Chase used two different radioactive isotopes: 32P to label DNA and 35S to label protein.
- By incorporating these isotopes into the DNA and protein of the T2 bacteriophage, they were able to track which molecule entered the bacterial cell during infection.

Infection Process:
- The T2 bacteriophage infects bacteria by injecting its genetic material into the host cell.
- By tracking the movement of the radioactive DNA and protein, Hershey and Chase could determine which molecule was responsible for carrying genetic information.

Results:
- They found that only the radioactive DNA entered the bacterial cell, while the protein remained outside.
- This demonstrated that DNA, not protein, is the molecule responsible for carrying genetic information.

Conclusion:
- Hershey and Chase's experiment provided strong evidence that DNA is the genetic material.
- Their findings were crucial in establishing the role of DNA in heredity and laid the foundation for future research in molecular biology.