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Which of the following is correct sequence of process of recombinant DNA technology-
i. Isolation of DNA
ii. Isolation of desired DNA fragment.
iii. Fragmentation of DNA by restriction endonuclease.
iv. Transferring of into host
v. Ligation of DNA fragment into vector
vi. Culturing in host cell to get desired product.
i. Isolation of DNA
ii. Isolation of desired DNA fragment.
iii. Fragmentation of DNA by restriction endonuclease.
iv. Transferring of into host
v. Ligation of DNA fragment into vector
vi. Culturing in host cell to get desired product.
- a)Step v, vi, iv, iii, ii and i
- b)Step i, ii, iii, iv, v and vi
- c)Step i, iii, ii, v, iv and vi
- d)Step i, iv, iii, ii, v and vi
Correct answer is option 'C'. Can you explain this answer?
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Which of the following is correct sequence of process of recombinant D...
The correct sequences of process of recombinant DNA technology are Isolation of DNA, fragmentation of DNA by restriction endonuclease, isolation of desired DNA fragment, ligation of DNA fragment into vector, transferring of into host and culturing in host cell to get desired product.
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Which of the following is correct sequence of process of recombinant D...
Recombinant DNA technology:
Recombinant DNA technology is a process in which DNA molecules from different sources are combined to create a new DNA molecule. This technology allows scientists to manipulate DNA sequences, clone genes, and produce large quantities of specific DNA fragments for various applications.
The correct sequence of the process of recombinant DNA technology is as follows:
i. Isolation of DNA:
The first step in recombinant DNA technology is to isolate the DNA of interest. This can be done by extracting DNA from a specific source, such as bacteria, plants, or animals.
ii. Isolation of desired DNA fragment:
Once the DNA is isolated, the desired DNA fragment is obtained using various techniques such as polymerase chain reaction (PCR) or restriction enzyme digestion. The DNA fragment may contain a specific gene or sequence of interest.
iii. Fragmentation of DNA by restriction endonuclease:
The isolated DNA is then fragmented using restriction endonucleases, which are enzymes that recognize specific DNA sequences and cut the DNA at those sites. This process generates DNA fragments with specific ends, which are necessary for subsequent steps.
iv. Transferring of DNA into host:
The fragmented DNA is then transferred into a host organism, which can be a bacterium, yeast, or other cell types. This is typically done using a technique called transformation, where the DNA is introduced into the host cells.
v. Ligation of DNA fragment into vector:
The DNA fragment is then ligated into a vector, which is a DNA molecule that can replicate independently in a host cell. The vector acts as a vehicle to carry the DNA fragment into the host cell and facilitate its replication.
vi. Culturing in host cell to get desired product:
The host cells containing the recombinant DNA are cultured under specific conditions to allow the replication and expression of the inserted DNA. This process leads to the production of the desired product, which can be a protein, enzyme, or any other desired molecule.
Therefore, the correct sequence of the process of recombinant DNA technology is i, iii, ii, v, iv, and vi.
Recombinant DNA technology is a process in which DNA molecules from different sources are combined to create a new DNA molecule. This technology allows scientists to manipulate DNA sequences, clone genes, and produce large quantities of specific DNA fragments for various applications.
The correct sequence of the process of recombinant DNA technology is as follows:
i. Isolation of DNA:
The first step in recombinant DNA technology is to isolate the DNA of interest. This can be done by extracting DNA from a specific source, such as bacteria, plants, or animals.
ii. Isolation of desired DNA fragment:
Once the DNA is isolated, the desired DNA fragment is obtained using various techniques such as polymerase chain reaction (PCR) or restriction enzyme digestion. The DNA fragment may contain a specific gene or sequence of interest.
iii. Fragmentation of DNA by restriction endonuclease:
The isolated DNA is then fragmented using restriction endonucleases, which are enzymes that recognize specific DNA sequences and cut the DNA at those sites. This process generates DNA fragments with specific ends, which are necessary for subsequent steps.
iv. Transferring of DNA into host:
The fragmented DNA is then transferred into a host organism, which can be a bacterium, yeast, or other cell types. This is typically done using a technique called transformation, where the DNA is introduced into the host cells.
v. Ligation of DNA fragment into vector:
The DNA fragment is then ligated into a vector, which is a DNA molecule that can replicate independently in a host cell. The vector acts as a vehicle to carry the DNA fragment into the host cell and facilitate its replication.
vi. Culturing in host cell to get desired product:
The host cells containing the recombinant DNA are cultured under specific conditions to allow the replication and expression of the inserted DNA. This process leads to the production of the desired product, which can be a protein, enzyme, or any other desired molecule.
Therefore, the correct sequence of the process of recombinant DNA technology is i, iii, ii, v, iv, and vi.
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Which of the following is correct sequence of process of recombinant D...
How can c option is right...explain any one
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Which of the following is correct sequence of process of recombinant DNA technology-i. Isolation of DNAii. Isolation of desired DNA fragment.iii. Fragmentation of DNA by restriction endonuclease.iv. Transferring of into hostv. Ligation of DNA fragment into vectorvi. Culturing in host cell to get desired product.a)Step v, vi, iv, iii, ii and ib)Step i, ii, iii, iv, v and vic)Step i, iii, ii, v, iv and vid)Step i, iv, iii, ii, v and viCorrect answer is option 'C'. Can you explain this answer?
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Which of the following is correct sequence of process of recombinant DNA technology-i. Isolation of DNAii. Isolation of desired DNA fragment.iii. Fragmentation of DNA by restriction endonuclease.iv. Transferring of into hostv. Ligation of DNA fragment into vectorvi. Culturing in host cell to get desired product.a)Step v, vi, iv, iii, ii and ib)Step i, ii, iii, iv, v and vic)Step i, iii, ii, v, iv and vid)Step i, iv, iii, ii, v and viCorrect answer is option 'C'. Can you explain this answer? for NEET 2025 is part of NEET preparation. The Question and answers have been prepared according to the NEET exam syllabus. Information about Which of the following is correct sequence of process of recombinant DNA technology-i. Isolation of DNAii. Isolation of desired DNA fragment.iii. Fragmentation of DNA by restriction endonuclease.iv. Transferring of into hostv. Ligation of DNA fragment into vectorvi. Culturing in host cell to get desired product.a)Step v, vi, iv, iii, ii and ib)Step i, ii, iii, iv, v and vic)Step i, iii, ii, v, iv and vid)Step i, iv, iii, ii, v and viCorrect answer is option 'C'. Can you explain this answer? covers all topics & solutions for NEET 2025 Exam. Find important definitions, questions, meanings, examples, exercises and tests below for Which of the following is correct sequence of process of recombinant DNA technology-i. Isolation of DNAii. Isolation of desired DNA fragment.iii. Fragmentation of DNA by restriction endonuclease.iv. Transferring of into hostv. Ligation of DNA fragment into vectorvi. Culturing in host cell to get desired product.a)Step v, vi, iv, iii, ii and ib)Step i, ii, iii, iv, v and vic)Step i, iii, ii, v, iv and vid)Step i, iv, iii, ii, v and viCorrect answer is option 'C'. Can you explain this answer?.
Which of the following is correct sequence of process of recombinant DNA technology-i. Isolation of DNAii. Isolation of desired DNA fragment.iii. Fragmentation of DNA by restriction endonuclease.iv. Transferring of into hostv. Ligation of DNA fragment into vectorvi. Culturing in host cell to get desired product.a)Step v, vi, iv, iii, ii and ib)Step i, ii, iii, iv, v and vic)Step i, iii, ii, v, iv and vid)Step i, iv, iii, ii, v and viCorrect answer is option 'C'. Can you explain this answer? for NEET 2025 is part of NEET preparation. The Question and answers have been prepared according to the NEET exam syllabus. Information about Which of the following is correct sequence of process of recombinant DNA technology-i. Isolation of DNAii. Isolation of desired DNA fragment.iii. Fragmentation of DNA by restriction endonuclease.iv. Transferring of into hostv. Ligation of DNA fragment into vectorvi. Culturing in host cell to get desired product.a)Step v, vi, iv, iii, ii and ib)Step i, ii, iii, iv, v and vic)Step i, iii, ii, v, iv and vid)Step i, iv, iii, ii, v and viCorrect answer is option 'C'. Can you explain this answer? covers all topics & solutions for NEET 2025 Exam. Find important definitions, questions, meanings, examples, exercises and tests below for Which of the following is correct sequence of process of recombinant DNA technology-i. Isolation of DNAii. Isolation of desired DNA fragment.iii. Fragmentation of DNA by restriction endonuclease.iv. Transferring of into hostv. Ligation of DNA fragment into vectorvi. Culturing in host cell to get desired product.a)Step v, vi, iv, iii, ii and ib)Step i, ii, iii, iv, v and vic)Step i, iii, ii, v, iv and vid)Step i, iv, iii, ii, v and viCorrect answer is option 'C'. Can you explain this answer?.
Solutions for Which of the following is correct sequence of process of recombinant DNA technology-i. Isolation of DNAii. Isolation of desired DNA fragment.iii. Fragmentation of DNA by restriction endonuclease.iv. Transferring of into hostv. Ligation of DNA fragment into vectorvi. Culturing in host cell to get desired product.a)Step v, vi, iv, iii, ii and ib)Step i, ii, iii, iv, v and vic)Step i, iii, ii, v, iv and vid)Step i, iv, iii, ii, v and viCorrect answer is option 'C'. Can you explain this answer? in English & in Hindi are available as part of our courses for NEET.
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