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In animal cell culture, a CO2 enriched atmosphere in the incubator chamber is used to maintain the culture pH between 6.9 and 7.4. Which one of the following statements is CORRECT?
- a)Higher the bicarbonate concentration in the medium, higher should be the requirement of gaseous CO2
- b)Lower the bicarbonate concentration in the medium, higher should be the requirement of gaseous CO2
- c)Higher the bicarbonate concentration in the medium, lower should be the requirement of gaseous CO2
- d)CO2 requirement is independent of bicarbonate concentration in the medium
Correct answer is option 'C'. Can you explain this answer?
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Verified Answer
In animal cell culture, a CO2 enriched atmosphere in the incubator cha...
The optimal pH range of 7.2 to 7.4 can be maintained by supplementing the medium with sodium bicarbonate and regulating the level of CO2 in the atmosphere above the medium as shown by the reaction below: H2O + CO2 + NaHCO3 H+ + Na+ + 2HCO3 – In tissue culture, cells are grown either in open systems (where there is free exchange of the atmosphere immediately above the medium with the atmosphere of the incubator) or in closed systems (where the two atmospheres are kept separate). The buffering system employed in the medium needs to be matched to the culture system. Otherwise the cells may be subject to metabolic stress which will impair their performance. In closed systems the level of CO2 is regulated by the metabolism of the cells. The culture vessel must be sealed (flasks tightly capped) to retain any CO2 generated by the cells.
Consequently, closed systems provide additional protection against contamination and have simpler incubator requirements than open systems. Closed systems usually require media with buffers based on Hanks’ balanced salt solution having relatively low levels of sodium bicarbonate. In open systems, humidity (to reduce evaporation) and a means of regulating CO2 levels (if the culture medium contains sodium bicarbonate) are required during incubation to maintain the pH of the culture medium. Open systems usually require the higher levels of sodium
bicarbonate found in Earle’s salt solution combined with a 5 to 10% CO2 atmosphere supplied by the incubator. In general, 1.2 g/L to 2.2 g/L of sodium bicarbonate is used with 5% CO2 whereas 3.7 g/L sodium bicarbonate is used with 10% CO2 . The exact amount will depend upon the medium formulation.
Consequently, closed systems provide additional protection against contamination and have simpler incubator requirements than open systems. Closed systems usually require media with buffers based on Hanks’ balanced salt solution having relatively low levels of sodium bicarbonate. In open systems, humidity (to reduce evaporation) and a means of regulating CO2 levels (if the culture medium contains sodium bicarbonate) are required during incubation to maintain the pH of the culture medium. Open systems usually require the higher levels of sodium
bicarbonate found in Earle’s salt solution combined with a 5 to 10% CO2 atmosphere supplied by the incubator. In general, 1.2 g/L to 2.2 g/L of sodium bicarbonate is used with 5% CO2 whereas 3.7 g/L sodium bicarbonate is used with 10% CO2 . The exact amount will depend upon the medium formulation.
Most Upvoted Answer
In animal cell culture, a CO2 enriched atmosphere in the incubator cha...
Explanation:
The presence of CO2 in the incubator chamber is necessary to maintain the pH of the culture medium between 6.9 and 7.4. The CO2 is required to form carbonic acid in the culture medium, which acts as a buffer and helps to maintain the pH within the desired range. The concentration of bicarbonate in the medium also affects the pH stability of the medium.
Higher the bicarbonate concentration in the medium, lower should be the requirement of gaseous CO2:
This is because bicarbonate is a buffering agent that helps to maintain the pH of the medium. When there is a higher concentration of bicarbonate, it acts as a buffer and reduces the requirement of gaseous CO2 to maintain the pH within the desired range.
Lower the bicarbonate concentration in the medium, higher should be the requirement of gaseous CO2:
This is because when there is a lower concentration of bicarbonate, there is less buffering capacity in the medium, and hence, more gaseous CO2 is required to maintain the pH within the desired range.
CO2 requirement is independent of bicarbonate concentration in the medium:
This statement is not correct because the presence of bicarbonate in the medium affects the buffering capacity of the medium, which in turn affects the requirement of gaseous CO2 to maintain the pH within the desired range.
Therefore, the correct statement is that higher the bicarbonate concentration in the medium, lower should be the requirement of gaseous CO2.
The presence of CO2 in the incubator chamber is necessary to maintain the pH of the culture medium between 6.9 and 7.4. The CO2 is required to form carbonic acid in the culture medium, which acts as a buffer and helps to maintain the pH within the desired range. The concentration of bicarbonate in the medium also affects the pH stability of the medium.
Higher the bicarbonate concentration in the medium, lower should be the requirement of gaseous CO2:
This is because bicarbonate is a buffering agent that helps to maintain the pH of the medium. When there is a higher concentration of bicarbonate, it acts as a buffer and reduces the requirement of gaseous CO2 to maintain the pH within the desired range.
Lower the bicarbonate concentration in the medium, higher should be the requirement of gaseous CO2:
This is because when there is a lower concentration of bicarbonate, there is less buffering capacity in the medium, and hence, more gaseous CO2 is required to maintain the pH within the desired range.
CO2 requirement is independent of bicarbonate concentration in the medium:
This statement is not correct because the presence of bicarbonate in the medium affects the buffering capacity of the medium, which in turn affects the requirement of gaseous CO2 to maintain the pH within the desired range.
Therefore, the correct statement is that higher the bicarbonate concentration in the medium, lower should be the requirement of gaseous CO2.
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In animal cell culture, a CO2 enriched atmosphere in the incubator chamber is used to maintain the culture pH between 6.9 and 7.4. Which one of the following statements is CORRECT?a)Higher the bicarbonate concentration in the medium, higher should be the requirement of gaseous CO2b)Lower the bicarbonate concentration in the medium, higher should be the requirement of gaseous CO2c)Higher the bicarbonate concentration in the medium, lower should be the requirement of gaseous CO2d)CO2 requirement is independent of bicarbonate concentration in the mediumCorrect answer is option 'C'. Can you explain this answer?
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In animal cell culture, a CO2 enriched atmosphere in the incubator chamber is used to maintain the culture pH between 6.9 and 7.4. Which one of the following statements is CORRECT?a)Higher the bicarbonate concentration in the medium, higher should be the requirement of gaseous CO2b)Lower the bicarbonate concentration in the medium, higher should be the requirement of gaseous CO2c)Higher the bicarbonate concentration in the medium, lower should be the requirement of gaseous CO2d)CO2 requirement is independent of bicarbonate concentration in the mediumCorrect answer is option 'C'. Can you explain this answer? for GATE 2024 is part of GATE preparation. The Question and answers have been prepared according to the GATE exam syllabus. Information about In animal cell culture, a CO2 enriched atmosphere in the incubator chamber is used to maintain the culture pH between 6.9 and 7.4. Which one of the following statements is CORRECT?a)Higher the bicarbonate concentration in the medium, higher should be the requirement of gaseous CO2b)Lower the bicarbonate concentration in the medium, higher should be the requirement of gaseous CO2c)Higher the bicarbonate concentration in the medium, lower should be the requirement of gaseous CO2d)CO2 requirement is independent of bicarbonate concentration in the mediumCorrect answer is option 'C'. Can you explain this answer? covers all topics & solutions for GATE 2024 Exam. Find important definitions, questions, meanings, examples, exercises and tests below for In animal cell culture, a CO2 enriched atmosphere in the incubator chamber is used to maintain the culture pH between 6.9 and 7.4. Which one of the following statements is CORRECT?a)Higher the bicarbonate concentration in the medium, higher should be the requirement of gaseous CO2b)Lower the bicarbonate concentration in the medium, higher should be the requirement of gaseous CO2c)Higher the bicarbonate concentration in the medium, lower should be the requirement of gaseous CO2d)CO2 requirement is independent of bicarbonate concentration in the mediumCorrect answer is option 'C'. Can you explain this answer?.
In animal cell culture, a CO2 enriched atmosphere in the incubator chamber is used to maintain the culture pH between 6.9 and 7.4. Which one of the following statements is CORRECT?a)Higher the bicarbonate concentration in the medium, higher should be the requirement of gaseous CO2b)Lower the bicarbonate concentration in the medium, higher should be the requirement of gaseous CO2c)Higher the bicarbonate concentration in the medium, lower should be the requirement of gaseous CO2d)CO2 requirement is independent of bicarbonate concentration in the mediumCorrect answer is option 'C'. Can you explain this answer? for GATE 2024 is part of GATE preparation. The Question and answers have been prepared according to the GATE exam syllabus. Information about In animal cell culture, a CO2 enriched atmosphere in the incubator chamber is used to maintain the culture pH between 6.9 and 7.4. Which one of the following statements is CORRECT?a)Higher the bicarbonate concentration in the medium, higher should be the requirement of gaseous CO2b)Lower the bicarbonate concentration in the medium, higher should be the requirement of gaseous CO2c)Higher the bicarbonate concentration in the medium, lower should be the requirement of gaseous CO2d)CO2 requirement is independent of bicarbonate concentration in the mediumCorrect answer is option 'C'. Can you explain this answer? covers all topics & solutions for GATE 2024 Exam. Find important definitions, questions, meanings, examples, exercises and tests below for In animal cell culture, a CO2 enriched atmosphere in the incubator chamber is used to maintain the culture pH between 6.9 and 7.4. Which one of the following statements is CORRECT?a)Higher the bicarbonate concentration in the medium, higher should be the requirement of gaseous CO2b)Lower the bicarbonate concentration in the medium, higher should be the requirement of gaseous CO2c)Higher the bicarbonate concentration in the medium, lower should be the requirement of gaseous CO2d)CO2 requirement is independent of bicarbonate concentration in the mediumCorrect answer is option 'C'. Can you explain this answer?.
Solutions for In animal cell culture, a CO2 enriched atmosphere in the incubator chamber is used to maintain the culture pH between 6.9 and 7.4. Which one of the following statements is CORRECT?a)Higher the bicarbonate concentration in the medium, higher should be the requirement of gaseous CO2b)Lower the bicarbonate concentration in the medium, higher should be the requirement of gaseous CO2c)Higher the bicarbonate concentration in the medium, lower should be the requirement of gaseous CO2d)CO2 requirement is independent of bicarbonate concentration in the mediumCorrect answer is option 'C'. Can you explain this answer? in English & in Hindi are available as part of our courses for GATE.
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In animal cell culture, a CO2 enriched atmosphere in the incubator chamber is used to maintain the culture pH between 6.9 and 7.4. Which one of the following statements is CORRECT?a)Higher the bicarbonate concentration in the medium, higher should be the requirement of gaseous CO2b)Lower the bicarbonate concentration in the medium, higher should be the requirement of gaseous CO2c)Higher the bicarbonate concentration in the medium, lower should be the requirement of gaseous CO2d)CO2 requirement is independent of bicarbonate concentration in the mediumCorrect answer is option 'C'. Can you explain this answer?, a detailed solution for In animal cell culture, a CO2 enriched atmosphere in the incubator chamber is used to maintain the culture pH between 6.9 and 7.4. Which one of the following statements is CORRECT?a)Higher the bicarbonate concentration in the medium, higher should be the requirement of gaseous CO2b)Lower the bicarbonate concentration in the medium, higher should be the requirement of gaseous CO2c)Higher the bicarbonate concentration in the medium, lower should be the requirement of gaseous CO2d)CO2 requirement is independent of bicarbonate concentration in the mediumCorrect answer is option 'C'. Can you explain this answer? has been provided alongside types of In animal cell culture, a CO2 enriched atmosphere in the incubator chamber is used to maintain the culture pH between 6.9 and 7.4. Which one of the following statements is CORRECT?a)Higher the bicarbonate concentration in the medium, higher should be the requirement of gaseous CO2b)Lower the bicarbonate concentration in the medium, higher should be the requirement of gaseous CO2c)Higher the bicarbonate concentration in the medium, lower should be the requirement of gaseous CO2d)CO2 requirement is independent of bicarbonate concentration in the mediumCorrect answer is option 'C'. Can you explain this answer? theory, EduRev gives you an
ample number of questions to practice In animal cell culture, a CO2 enriched atmosphere in the incubator chamber is used to maintain the culture pH between 6.9 and 7.4. Which one of the following statements is CORRECT?a)Higher the bicarbonate concentration in the medium, higher should be the requirement of gaseous CO2b)Lower the bicarbonate concentration in the medium, higher should be the requirement of gaseous CO2c)Higher the bicarbonate concentration in the medium, lower should be the requirement of gaseous CO2d)CO2 requirement is independent of bicarbonate concentration in the mediumCorrect answer is option 'C'. Can you explain this answer? tests, examples and also practice GATE tests.
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